Abstract
A procedure is developed for the determination of citrate ions in blood plasma by capillary zone electrophoresis at a level of 20‒800 mg/L. The background electrolyte (BGE) was a solution containing 10 mM chromate ions and 40 mM diethanolamine. Sample preparation included protein precipitation with acetonitrile, followed by dilution with a solution containing an internal standard (fluoride ion), and centrifugation. It is shown that the use of the internal standard ensures the account of the change in the migration time of the analyte in the analysis of biological samples. The relative standard deviation of analysis does not exceed 10% for two replicate determinations. The systematic error of the developed procedure was evaluated by the added–found method.
Similar content being viewed by others
REFERENCES
Strokov, A.G. and Poz, Ya.L., Vestn. Transplantol. Iskusstv. Organov, 2010, vol. 12, no. 4. C. 80.
Davenport, A. and Tolwani, A., Clin. Kidney J., 2009, vol. 2, no. 6, p. 439.
Kent, J.C., Arthur, P.A., and Hartmann, P.E., J. Dairy Res., 1998, vol. 65, p. 55.
Zarei, K., Atabati, M., and Karimian, N., J. Anal. Chem., 2008, vol. 63, no. 2, p. 145.
Naumenko, L.F., Buneeva, N.M., Korneeva, R.N., Selemenev, V.F., and Nemtsev, D.V., J. Anal. Chem., 2004, vol. 59, no. 3, p. 291.
Tkachenko, S.V., Naumenko, L.F., Korneeva, R.N., Buneeva, N.M., and Selemenev, V.F., Sorbtsionnye Khromatogr. Protsessy, 2010, vol. 10, no. 6, p. 901.
GOST (State Standard) 32743-2014: Food Additives. Method of Potentiometric Determination of Citric Acid and Citric Ions in Complex Food Additives, Moscow: Standartinform, 2015.
Hoppe, B., Kemper, M.J., Hvizd, M.G., Sailer, D.E., and Langman, G.B., Kidney Int., 1998, vol. 53, p. 1348.
Savchenko, L.N. and Nikitina, T.G., Zavod. Lab., Diagn. Mater., 2008, vol. 74, no. 9, p. 18.
Rivasseau, C., Boisson, A.-M., Mongélard, G., Couram, G., Bastien, O., and Blign, R., J. Chromatogr. A, 2006, vol. 1129, p. 283.
Dolnik, V. and Dolnikova, J., J. Chromatogr. A, 1995, vol. 716, p. 269.
Sidorova, A.A. and Grigor’ev, A.V., J. Anal. Chem., 2012, vol. 67, no. 5, p. 478.
Polyakova, E.V., Shuvaeva, O.V., and Polyanskaya, E.M., Analitika Kontrol’, 2005, vol. 9, no. 1, p. 70.
Komarova, N.V. and Kamentsev, Ya.S., Prakticheskoe rukovodstvo po ispol’zovaniyu sistem kapillyarnogo elektroforeza Kapel’ (Practical Guide to the Use of Kapel’ Capillary Electrophoresis Systems), St. Petersburg: Veda, 2006.
Wildman, B.J., Jackson, P.E., Jones, W.R., and Alden, P.G., J. Chromatogr. A, 1991, vol. 546, p. 459.
Swartz, M.E., J. Chromatogr. A, 1993, no. 640, p. 441.
Holmes, R.P., Clin. Chem., 1995, no. 41, p. 1297.
Oefner, P.J., Electrophoresis, 1995, no. 16, p. 46.
Polyakova, E.V. and Shuvaeva, O.V., Analitika Kontrol’, 2009, vol. 13, no. 3, p. 147.
Sursyakova, V.V., Kalyakin, S.N., Burmakina, G.V., and Rubailo, A.I., J. Anal. Chem., 2012, vol. 67, no. 9, p. 783.
Zheng, Y., Xu, Z., Zhu, Q., Liu, J., Qian, J., You, H., Gu, Y., Hao, C., Jiao, Z., and Ding, F., PLoS One, 2013. http://journals.plos.org/plosone/article?id= 10.1371/journal.pone.0065992. Accessed February 20, 2017.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated by V. Kudrinskaya
Rights and permissions
About this article
Cite this article
Polyakova, E.V., Shuvaeva, O.V. & Borisov, A.S. Determination of Citrate Ions in Blood Plasma by Capillary Zone Electrophoresis. J Anal Chem 73, 906–909 (2018). https://doi.org/10.1134/S1061934818090113
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061934818090113