Abstract
A method for the determination of polycyclic aromatic hydrocarbons (PAHs) at the ppb level in waxes has been developed. It consists of single extraction of the aromatic fraction from 5 g of melted wax into 40 mL of acetonitrile, followed by partial evaporation of the solvent. Quantification of PAHs was performed by HPLC with fluorimetric detection (HPLC-FL) using two-channel programmable excitation and emission wavelengths. On-column limits of detection (LODs) were 0.1–1 pg. The average recovery of PAHs was 68%. Calculated LODs of PAHs in wax samples were 3–23 μg/kg. No interferences of small amounts of wax were observed during the FL detection. Selection of the best solvent was made by means of the Chemcad simulation of the extraction process. The most promising systems were verified experimentally. Other detection modes, such as ultraviolet, gas chromatography−mass spectrometric and gas chromatography with flame ionization, were compared with the proposed HPLC-FL.
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REFERENCES
Abdel-Shafy, H.I. and Mansour, M.S.M., Egypt.J. Pet., 2016, vol. 25, no. 1, p. 107.
Zelinkova, Z. and Wenzl, T., Polycyclic Aromat. Compd, 2015, vol. 35, nos. 2–4, p. 248.
Singh, L., Varshney, J.G., and Agarwal, T., Food Chem., 2016, vol. 199, p. 768.
Huang, Y., Wei, J., Song, J., Chen, M., and Luo, Y., Chemosphere, 2013, vol. 92, no. 8, p. 1010.
Bansal, V., Kumar, P., Kwon, E.E., and Kim, K.-H., Crit. Rev. Food Sci. Nutr., 2017, vol. 57, no. 15, p. 3297.
Cai, S.-S., Syage, J.A., Hanold, K.A., and Balogh, M.P., Anal. Chem., 2009, vol. 81, no. 6, p. 2123.
Xiaoli, Z., Eur. Chem. Bull., 2012, vol. 1, no. 16, p. 210.
Westneat, D.F., Anal. Chem., 1961, vol. 33, no. 6, p. 810.
Pandey, M.K., Mishra, K.K., Khanna, S.K., and Das, M., J. Am. Oil Chem. Soc., 2004, vol. 81, no. 12, p. 1131.
Shiraishi, Y. and Shirotori, T., Food Hyg.Saf. Sci., 1977, vol. 18, no. 5, p. 426.
Hopia, A., Pyysalo, H., and Wickstrom, K., J. Am. Oil Chem. Soc., 1986, vol. 63, no. 7, p. 889.
Fromberg, A., Højgård, A., and Duedahl-Olesen, L., Food Addit. Contam., 2007, vol. 24, no. 7, p. 758.
Jennings, D.W. and Weispfennig, K., Fluid Phase Equilib., 2005, vol. 227, no. 1, p. 27.
Funding
This research was supported by The National Centre for Research and Development (NCBiR) strategic program of research and development, “Environment, agriculture and forestry” – BIOSTRATEG “The new packaging with the use of renewable raw materials and innovative impregnating paraffin” (grant number BIOSTRATEG2/298537/7/ NCBR/2016).
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Tomasz Krawczyk, Czechowicz, D. & Iłowska, J. Determination of Polycyclic Aromatic Hydrocarbons in Waxes by High Performance Liquid Chromatography with Fluorimetric Detection. J Anal Chem 75, 495–502 (2020). https://doi.org/10.1134/S1061934820040073
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DOI: https://doi.org/10.1134/S1061934820040073