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Biocontrol Potential of Novel Borrelidin-Producing Streptomyces rochei 3IZ-6 Isolated from Soil

  • PHYSIOLOGICALLY ACTIVE SUBSTANCES OF SOIL MICROORGANISMS
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Abstract—

A complex of soil actinomycetes in the upper horizon of Grumusol (Vertisol) on the western coast of Lake Kinneret in the vicinity of Tiberias (Lower Galilee, Israel) was studied. A high-throughput screening of the antibiotic activity of 26 isolates of the genus Streptomyces using on a dual reporter system revealed the strain 3IZ-6, which had the ability to inhibit protein synthesis. This strain was assigned to Streptomyces rochei according to the polyphase taxonomy approach. The active substance of S. rochei 3IZ-6 was isolated and purified using solid-phase extraction and high-performance liquid chromatography. Toeprint analysis and mass spectrophotometric data allowed us to identify this active compound as borrelidin, a known inhibitor of protein biosynthesis. When co-cultivated on solid media, strain 3IZ-6 is able to suppress the growth of phytopathogenic microorganisms Fusarium solani, F. sambucinum, and Botrytis cinerea, as well as the quarantine object Curtobacterium flaccumfaciens. The cultural liquid of 3IZ-6 had a pronounced phytotoxic effect on the seeds of common wheat (Triticum aestivum L.). Strain Streptomyces rochei 3IZ-6 can find application in biotechnology as a producer of borrelidin, a valuable natural compound with a wide spectrum of antibiotic activity, which can be used as a biocontrol agent for crop protection from phytopathogens and weeds.

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ACKNOWLEDGMENTS

I.G. Shirokikh, A.A. Shirokikh, Ya.I. Nazarova, and N.A. Bokov are grateful to the Rudnitsky Federal Agricultural Research Center for support within the framework of state assignment of no. FNWE-2022-0005.

Yu.V. Zakalyukina thanks the Ministry of Science and Higher Education of the Russian Federation for support within the framework of state assignment no. 075-15-2021-1396.

The study of spore surface ultrastructure of S. rochei 3IZ-6 was carried out in the Laboratory of Electron Microscopy of the Faculty of Biology of the Lomonosov Moscow State University.

Funding

This study was supported by the Russian Science Foundation (project no. 22-24-00278).

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Authors and Affiliations

  1. Rudnitsky Federal Agricultural Research Center of the North-East, 610007, Kirov, Russia

    I. G. Shirokikh, A. A. Shirokikh, Ya. I. Nazarova & N. A. Bokov

  2. Lomonosov Moscow State University, 119991, Moscow, Russia

    I. A. Osterman, D. A. Lukianov, V. I. Marina, M. V. Biryukov & Yu. V. Zakalyukina

  3. Skolkovo Institute of Science and Technology, Center of Life Sciences, 121205, Moscow, Russia

    I. A. Osterman, D. A. Lukianov & V. I. Marina

  4. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, 117997, Moscow, Russia

    O. A. Belozerova & E. B. Guglya

  5. Pirogov Russian National Research Medical University, 117997, Moscow, Russia

    E. B. Guglya

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  1. I. G. Shirokikh
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Correspondence to Yu. V. Zakalyukina.

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Translated by D. Konyushkov

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Shirokikh, I.G., Osterman, I.A., Lukianov, D.A. et al. Biocontrol Potential of Novel Borrelidin-Producing Streptomyces rochei 3IZ-6 Isolated from Soil. Eurasian Soil Sc. 56, 619–627 (2023). https://doi.org/10.1134/S1064229323600161

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