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A new fluorescent analogue for the studies of anandamide transport in cell cultures

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Abstract

A new fluorescent analogue of anandamide bearing a BODIPY®-FL-fluorophore and linked to arachidonic acid via a 2,2′-(ethylenedioxy)-bis(ethylenediamine) residue was prepared. The fluorescent analogue was demonstrated to be a substrate of the cell anandamide uptake system (K m 4.5 ± 0.9 μM, V max 20 ± 1 amol/(min cell)) in rat glioma C6 cells.

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Abbreviations

AEA:

arachidonoyl ethanolamide

BODIPY®-FL-C3 :

4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacen-3-propionic acid

FAAH-1:

type 1 membrane-bound fatty-acid amide hydrolase

EDBE:

2,2′-(ethylenedioxy)-bis(ethylenediamine)

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 111997, Russia

    N. M. Gretskaya, M. G. Akimov & V. V. Bezuglov

Authors
  1. N. M. Gretskaya
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  2. M. G. Akimov
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  3. V. V. Bezuglov
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Corresponding author

Correspondence to V. V. Bezuglov.

Additional information

Original Russian Text © N.M. Gretskaya, M.G. Akimov, V.V. Bezuglov, 2014, published in Bioorganicheskaya Khimiya, 2014, Vol. 40, No. 2, pp. 248–252.

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Gretskaya, N.M., Akimov, M.G. & Bezuglov, V.V. A new fluorescent analogue for the studies of anandamide transport in cell cultures. Russ J Bioorg Chem 40, 228–232 (2014). https://doi.org/10.1134/S1068162014020046

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  • DOI: https://doi.org/10.1134/S1068162014020046

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