Abstract
We studied the morphological changes and expression of the astroglial and microglial markers in the sensorimotor cortex and hippocampus of rats that were subjected to chronic stress. Chronic neurotization was associated with the appearance of a large number of damaged neurons in layer V of the sensorimotor cortex and the pyramidal layer of the hippocampus. These changes were considerably expressed in the CA3 field. A neurosis-like state in rats was accompanied by an increased expression of microglial markers in the hippocampus and this effect was evident even 1 month after the end of stress. The expression of the astroglial marker GFAP decreased in the CA3 hippocampal field. Treatment of animals with the pantothenic acid derivative panthenol stabilized the glial response to chronic stress; however, its effects were not long-lasting and did not prevent activation of microglia during the period after stress.
Similar content being viewed by others
References
Hecht, K. and Poppei, M., Zur Rolle des Umweltfaktors in der dialektischen Gesundheits-Krankheitsbeziehung eines Organismus, Berlin: Forschungsverband Herz-Kreislaufkrankheiten, 1977.
Baumann, R. and Hecht, K., Stress, Neurose und Herz-Kreislauf, Berlin: VEB Deutscher Verlag der Wissenschaften, 1977.
Levina, O.L., Dresher, Yu., Gulyaeva, N.V., and Levshina, I.P., Zh. Vyssh. Nervn. Deyat. im. I.P. Pavlova, 1985, vol. 35, no. 4, pp. 764–769.
Gulyaeva, N.V. and Levshina, I.P., Byull. Eksperim. Biol. Med, 1988, vol. 106, no. 8, pp. 153–156.
Aleksandrovskaya, M.M. and Kol’tsova, A.V., Zh. Vyssh. Nervn. Deyat. im. I.P. Pavlova, 1980, vol. 30, no. 4, pp. 747–754.
Airapetyants, M.G., Zh. Vyssh. Nervn. Deyat. im. I.P. Pavlova, 1997, vol. 47, no. 2, pp. 412–417.
Airapetyants, M.G., Zh. Vyssh. Nervn. Deyat. im. I.P. Pavlova, 2005, vol. 55, no. 6, pp. 734–746.
Airapetyants, M.G., Yakovlev, A.A., Levshina, I.P., Vorontsova, O.N., Stepanichev, M.Yu., Onufriev, M.V., Lazareva, N.A., and Gulyaeva, N.V., Neirokhimiya, 2006, vol. 23, no. 2, pp. 136–142.
Gulyaeva, N.V., Neirokhimiya, 1984, vol. 3, no. 3, pp. 260–268.
Levshina, I.P. and Gulyaeva, N.V., Zh. Vyssh. Nerv. Deyat. im. I.P. Pavlova, 1984, vol. 34, no. 5, pp. 967–971.
Aleksandrovskaya, M.M. and Kol’tsova, A.V., Zh. Vyssh. Nervn. Deyat. im. I.P. Pavlova, 1978, vol. 28, no. 3, pp. 529–536.
Gulyaeva, N.V. and Levshina, I.P., Zh. Vyssh. Nervn. Deyat. im. I.P. Pavlova, 1984, vol. 34, no. 3, pp. 554–559.
Vein, A.M., Lektsii po patologii vegetativnoi nervnoi sistemy (Lectures on pathology of the autonomic nervous system), Moscow: Meditsina, 1981.
Kucherenko, R.P., Usova, I.P., and Gilerovich, E.G., Zh. Vyssh. Nervn. Deyat. im. I.P. Pavlova, 1983, vol. 33, no. 3, pp. 508–513.
Manina, A.A., Khananashvili, M.M., and Lazurko, N.M., Zh. Vyssh. Nervn. Deyat. im. I.P. Pavlova, 1971, vol. 21, no. 4, pp. 686–691.
Suvorov, N.F., Makarov, F.N., and Taranova, N.P., Nevrozy: eksperimental’nye i klinicheskie issledovaniya (Neurosis: experimental and clinical studies), Leningrad: Nauka, 1989, pp. 55–66.
Tishkina, A.O., Levshina, I.P., Lazareva, N.A., Pasikova, N.V., Stepanichev, M.Yu., Airapetyants, M.G., and Gulyaeva, N.V., Dokl. Akad. Nauk, 2009, vol. 428, pp. 130–134.
Tishkina, A., Rukhlenko, A., Stepanichev, M., Levshina, I., Pasikova, N., Onufriev, M., Moiseeva, Y., Piskunov, A., and Gulyaeva, N., Metab. Brain Dis., 2012, vol. 27, pp. 431–441.
Paxinos, G. and Watson, C., The Rat Brain in Stereotaxic Coordinates, Sidney: Academ. Press, 1982.
Streit, W.J., J. Histochem. Cytochem., 1990, vol. 38, pp. 1683–1686.
Tishkina, A.O., Neirokhimimya, 2009, vol. 26, no. 4, pp. 341–346.
Sofroniew, M.V. and Vinters, H.V., Acta Neuropathol., 2010, vol. 119, pp. 7–35.
Graeber, M.B. and Streit, W.J., Acta Neuropathol., 2010, vol. 119, pp. 89–105.
Moiseenok, A.G., Pantotenovaya kislota (biokhimiya i primenenie vitamina) (Pantothenic acid (biochemistry and application of the vitamin)), Minsk: Nauka i tekhnika, 1980.
Moiseenok, A.G., Kopelevich, V.M., Sheibak, V.M., and Gurinovich, V.A., Proizvodnye pantotenovoi kisloty (Derivatives of pantothenic acid), Minsk: Nauka i tekhnika, 1989.
Depeint, F., Bruce, W.R., Shangari, N., Mehta, R., and O’Brien, P.J., Chem. Biol. Interact., 2006, vol. 16, pp. 394–112.
Moiseenok, A.G., Gurinovich, V.A., Omel’yanchik, S.N., and Slyshenkov, V.S., Ukr. Biokhim. Zhurn., 2004, vol. 76, pp. 68–81.
Magarinos, A.M. and McEwen, B.S., Neuroscience, pp. 89–98.
Arbel, I., Kadar, T., Silberman, M., and Levy, A., Brain Res., 1994, vol. 657, pp. 227–235.
Mizoguchi, K., Kunishita, T., Chui, D.-H., and Tabira, T., Neurosci. Let., 1992, vol. 138, pp. 157–160.
Conrad, C.D., LeDoux, J.E., Magarinos, A.M., and McEwen, B.S., Behav. Neurosci., 1999, vol. 113, pp. 902–913.
Miyata, S., Koyama, Y., Takemoto, K., Yoshikawa, K., Ishikawa, T., Taniguchi, M., Inoue, K., Aoki, M., Hori, O., Katayama, T., and Tohyama, M., PLoS One, 2011, vol. 6, no. 5: e19859.
Braun, K., Antemano, R., Helmeke, C., Büchner, M., and Poeggel, G., Neuroscience, 2009, vol. 160, pp. 629–638.
Musholt, K., Cirillo, G., Cavaliere, C., Rosaria, Rosaria-Bianco, M., Bock, J., Helmeke, C., Braun, K., and Papa, M., Dev. Neurobiol., 2009, vol. 69, pp. 203–211.
Imbe, H., Kimura, A., Donishi, T., and Kaneoke, Y., Neuroscience, 2012, vol. 223, pp. 209–218.
McEwen, B.S., Conrad, C.D., Kuroda, Y., Frankfurt, M., Magarinos, A.M., and McKittrick, C., Eur. Neuropsychiatry, 1997, vol. 7, pp. S323–S328.
Nair, A. and Bonneau, R.H., J. Neuroimmunol., 2006, vol. 171, pp. 72–85.
Kwon, M.S., Seo, Y.J., Lee, J.K., Lee, H.K., Jung, J.S., Jang, J.E., Park, S.H., and Suh, H.W., Neurosci. Let., 2008, vol. 430, pp. 258–263.
Lino-de Oliveira, C. and de Oliveira, R.M., Padua Carobrez A., de Lima T.C., del Bel, E.A., Guimaraes F.S, Brain. Res. Bull., 2006, vol. 70, pp. 414–421.
Sugama, S., Fujita, M., Hashimoto, M., and Conti, B., Neuroscience, 2007, vol. 146, pp. 1388–1399.
Sugama, S., Takenouchi, T., Fujita, M., Conti, B., and Hashimoto, M., J. Neuroimmunol., 2009, vol. 207, pp. 24–31.
Drew, G.M., Mitchell, V.A., and Vaughan, C.W., J. Neurosci., 2008, vol. 28, pp. 808–815.
Manzhulo, I.V., Ogurtsova, O.S., Dyuizen, I.V., and Lamash, N.E., Neirokhimiya, 2013, vol. 30, no. 1.
Slyshenkov, V.S., Omelyanchik, S.N., Moiseenok, A.G., Trebukhina, R.V., and Wojtczak, L., Free Radic. Biol. Med., 1998, vol. 24, pp. 894–899.
Moiseenok, A.G., Biokhimiya, farmakologiya i klinicheskoe primenenie proizvodnykh pantotenovoi kisloty. Sb. nauchnykh statei. (Biochemistry, Pharmacology and Application of the Derivatives of Pantothenic Acid. Collection of Scientific Works), Ed., Moiseenk, A.G., Grodno: Institute biokhimii NANB, 2003, pp. 107–114.
Moiseenok, A.G., Komar, V.I., Khomich, T.I., Kanunnikova, N.P., and Slyshenkov, V.S., Biofactors, 2000, vol. 11, pp. 53–55.
Fernandez-Fernandez, S., Almeida, A., and Bolaños, J.P., Biochem. J., 2012, vol. 443, pp. 3–11.
Mats, V.N., Neirono-glial’nye sootnosheniya v neokortekse krys pri obuchenii (Neurons-Glia relationships in the neocortex of rats during learning), Moscow: Nauka, 1994.
Barres, B.A., J. Neurosci., 1991, vol. 11, pp. 3684–3695.
Hertz, L., Brain Res. Bull., 1992, vol. 29, pp. 303–313.
Laming, P.R., Comp. Biochem. Physiol., 1989, vol. 94A, pp. 555–568.
Smith, S.J., Prog. Brain Res., 1992, vol. 94, pp. 119–136.
Hertz, L., Gibbs, M.E., O’dowd, B.S., Sedman, G.L., Robinson, S.R., Sykova, E., Hajek, I., Hertz, E., Peng, L., Huang, R., and Ng, K.T., Neurosci. Biobehav. Rev., 1996, vol. 20, pp. 537–551.
Caudle, R.M., Theor. Biol. Med. Model, 2006, vol. 3: 2.
Henneberger, C., Papouin, T., Oliet, S.H., and Rusakov, D.A., Nature, 2010, vol. 463, pp. 232–236.
Navarrete, M. and Perea, G., Fernandez de Sevilla D., Gómez-Gonzalo M., Núñez A., Martín E.D., Araque A, PLoS Biol., 2012, vol. 10, no. 2: e1001259.
Tian, L., Rauvala, H., and Gahmberg, C.G., Trends Immunol., 2009, vol. 30, pp. 91–99.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.O. Tishkina, M.R. Novikova, M.Yu. Stepanichev, I.P. Levshina, N.V. Pasikova, N.A. Lazareva, A.G. Moiseenok, N.V. Gulyaeva, 2013, published in Neirokhimiya, 2013, Vol. 30, No. 2, pp. 158–167.
Rights and permissions
About this article
Cite this article
Tishkina, A.O., Novikova, M.R., Stepanichev, M.Y. et al. Changes in the expression of astroglial and microglial markers in the hippocampus of rats adapted to chronic stress and the effects of panthenol. Neurochem. J. 7, 150–158 (2013). https://doi.org/10.1134/S1819712413020074
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1819712413020074