Abstract—
Neurogenesis is a complex process which governs embryonic brain development and is important for brain plasticity throughout the whole life. Postnatal neurogenesis occurs in neurogenic niches that regulate the processes of proliferation and differentiation of stem and progenitor cells driven by stimuli triggering mechanisms of neuroplasticity. In the neurogenic niches cells of glial and endothelial origin are the key regulators of neurogenesis. It is known that physiological neurogeneses is crucial for memory formation, whereas reparative neurogenesis provides partial repair of injured brain structures and compensation of neurological deficits caused by the brain injury. Dysregulation of neurogenesis is a characteristics feature of various neurodevelopmental and neurodegenerative diseases, particularly, Alzheimer’s disease representing a very important medical and social problem. In the in vitro model of the neurogenic niche using hippocampal neurospheres as a source of stem/progenitor cells and astrocytes, we have studied effects of astrocyte activation on the expression of markers of different stages of cell proliferation and differentiation. We found that aberrant mechanisms of development of stem and progenitor cells, caused by the beta-amyloid peptide (Аβ1–42), can be partially restored by targeted activation of GFAP-expressing cells in the neurogenic niche.
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ACKNOWLEDGMENTS
The authors thank Professor S. Kasparov (University of Bristol, UK) for providing adenoviral vectors.
Funding
This work was performed within the State Assignment of the Ministry of Public Health of the Russian Federation (2018–2020) “New Technologies for the Management of Neurogenesis and Angiogenesis in the Brain.”
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Animal experiments were carried out in accordance with generally accepted ethical international standards and the principles of humanity stated in the European Community Directive (2010/63/EC) and the requirements of the order of the Ministry of Public Health of the Russian Federation no. 267 of June 19, 2003 “On the Approval of Laboratory Practice in the Russian Federation.”
The authors declare that they have no conflict of interest.
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Translated by A. Medvedev
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Morgun, A.V., Osipova, E.D., Boytsova, E.B. et al. Astrocyte-Mediated Regulation of Cell Development in the Model of Neurogenic Niche in Vitro Treated with Aβ1-42. Biochem. Moscow Suppl. Ser. B 14, 6–14 (2020). https://doi.org/10.1134/S1990750820010114
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DOI: https://doi.org/10.1134/S1990750820010114