Abstract
A modern molecular genetic method has been adapted to study microorganisms associated with fish. A procedure for total DNA isolation from various organs and tissues of fish has been developed. Besides fish freshly caught, frozen and salted specimens were used. A bacterial PCR product was shown to amplify with highly conservative primers at an annealing temperature of 70–72°C from fish gills, liver, intestine, and skin.
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Original Russian Text © N.L. Bel’kova, E.V. Dzyuba, E.V. Sukhanova, T.A. Khanaeva, 2008, published in Biologiya Vnutrennikh Vod, No. 2, 2008, pp. 91–94.
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Bel’kova, N.L., Dzyuba, E.V., Sukhanova, E.V. et al. Adaptation of molecular genetic methods to study microorganisms associated with fish. Inland Water Biol 1, 192–195 (2008). https://doi.org/10.1134/S1995082908020120
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DOI: https://doi.org/10.1134/S1995082908020120