Abstract
Genetic engineering allows modification of bacterial and bacteriophage genes, which code for surface proteins, enabling display of random peptides on the surface of these microbial vectors. Biologic peptide libraries thus formed are used for high-throughput screening of clones bearing peptides with high affinity for target proteins. There are reports of many successful affinity selections performed with phage display libraries and substantially fewer cases describing the use of bacterial display systems. In theory, bacterial display has some advantages over phage display, but the two systems have never been experimentally compared. We tested both techniques in selecting streptavidin-binding peptides from two commercially available libraries. Under similar conditions, selection of phage-displayed peptides to model protein streptavidin proved convincingly better.
Similar content being viewed by others
References
Scott, J. K. and Smith, G. P. (1990), Science 249, 386–390.
Lu, Z., Murray, K. S., Van Cleave, V., La Vallie, E. R., Stahl, M. L., and McCoy, J. M. (1995), Biotechnology 13(4), 366–372.
Smith, G. P. and Petrenko, V. A. (1997), Chem. Rev. 97, 391–410.
Dedova, O., Fletcher, P., Liu, H., Wang, P., Blume, A., Brissette, R., Hsiao, K., Lennick, M., Pillutla, R., and Goldstein, N. (2004), Patent no. US2004023887.
McConnell, S. J. and Spinella, D. G. (1999), Patent no. WO9947151.
Hyde-DeRuyscher, R., Paige, L. A., Christensen, D. J., et al. (2000), Chem. Biol. 7 (1), 17–25.
Kay, B. K. and Hamilton, P. T. (2001), Comb. Chem. High Through. Screen. 4, 535–543.
Markland, W., Roberts, B. L., and Ladner, R. C. (1996), in Methods in Enzymology, vol. 267, Abelson, J. N. ed., Academic, New York, pp. 28–51.
Lunder, M., Bratkovič, T., Kreft, S., and Ŝtrukelj, B. (2005), J. Lipid Res. 46, 1512–1516.
Bratkovič, T., Lunder, M., Popovič, T., Kreft, S., Turk, B., Štrukelj, B., and Urleb, U. (2005), Biochem. Biophys. Res. Commun. 332, 897–903.
Sparks, A. B., Adey, N. B., Cwirla, S., and Kay, B. K. (1996), in Phage Display of Peptides and Proteins: A Laboratory Manual, Kay, B. K., Winter, J., and McCafferty, J., eds., Academic, San Diego, pp. 227–253.
Kay, B. K., Kasanov, J., and Yamabhai, M. (2001), Methods 24, 240–246.
Smith, G. P. and Scott, J. K. (1993), in Methods in Enzymology, vol. 217, Wu, R., ed., Academic, New York, pp. 228–257.
Cwirla, S. E., Peters, E. A., Barrett, R. W., and Dower, W. J. (1990), Proc. Natl. Acad. Sci. USA 87, 6378–6382.
van Zonnenveld, A. J., van den Berg, B. M. M., van Meijer, M., and Pannekoek, H. (1995), Gene 167, 49–52.
Yu, H., Dong, X., and Sun Y. (2004), Biochem. Eng. J. 18, 169–175.
Brown, C. K., Modzelewski, R. A., Johnson, C. S., and Wong, M. K. K. (2000), Ann. Surg. Oncol. 7(10), 743–749.
Lu, Z., Tripp, B. C., and McCoy, J. M. (1998), Methods Mol. Biol. 87, 265–280.
Brown, S. (1997), nat. Biotechnol. 15(3), 269–272.
Tripp, B. C., Lu, Z., Bourque, K., Sookdeo, H., and McCoy, J. M. (2001), Protein Eng. 14(5), 367–377.
Khan, A. S., Thompson R., Cao, C., and Valdes, J. J. (2003), Biotechnol. Lett., 25(19), 1671–1675.
Hansson, M., Samuelson, P., Gunneriusson, E., and Stahl, S. (2001), Comb. Chem. High Through. Screen. 4(2), 171–184.
Zitzmann, S., Kramer, S., Mier, W., Mahmut, M., Fleig, J., Atmann, A., Eisenhut, M., and Haberkorn, U. (2005), J. Nucl. Med. 46(5), 782–785.
Giebel, L. B., Cass, R. T., Milligan, D. L., Young, D. C., Arze, R., and Johnson, C. R. (1995), Biochemistry 34(47), 15,430–15,435.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lunder, M., Bratkovič, T., Doljak, B. et al. Comparison of bacterial and phage display peptide libraries in search of target-binding motif. Appl Biochem Biotechnol 127, 125–131 (2005). https://doi.org/10.1385/ABAB:127:2:125
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/ABAB:127:2:125