Abstract
An indium tinoxide (ITO) electrode was chemically modified by one layer of viologen (VIO) deri vative, which possessed a persistent and reproducible electrochemical response. A monolayer of a thermal stable hydrogenase from Thiocapsa roseopersic ina was stabilized on a synthesized poly-l-lysine subphase surface and transferred on to the electrode for fabrication of an ITO-VIO-hydrogenase heterogeneous system. Electrochemical properties of both the ITO-VIO monolayer and the heterogeneous ITO-VIO-hydrogenase system have been investigated. Hydrogen evolution could be measured by potentiostating the VIO-hydrogenase-covered ITO electrode to “electroplate” [(VIO+)n]surf and a large increase in hydrogen evolution was observed when using an electrolytesolution containing sodium dithionite. We discuss the possible electron transfer process.
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Qian, DJ., Nakamura, C., Noda, K. et al. Fabrication of an electrode-viologen-hydrogenase heterogeneous system and the electrochemical hydrogen evolution. Appl Biochem Biotechnol 84, 409–418 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:409
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DOI: https://doi.org/10.1385/ABAB:84-86:1-9:409