Skip to main content
SpringerLink
Log in

Annexin 1 (lipocortin 1) mimics inhibitory effects of glucocorticoids on testosterone secretion and enhances effects of interleukin-1β

  • Published:
Endocrine Aims and scope Submit manuscript

Abstract

Annexin 1 is an important mediator of glucocorticoid action in the hypothalamo-pituitary axis; however, little is known of its role in mediating glucocorticoid actions in the peripheral endocrine organs. Accordingly, we have carried out a preliminary study to investigate the effects of annexin 1 in vitro on the testicular secretion of testosterone, a process inhibitied by both glucocorticoids and interleukin-1β (IL-1β). Luteinizing hormone (LH) and forskolin stimulated the release of testosterone from dispersed murine testicular cells in vitro. Their effects were reduced in cells from mice pretreated with dexamethasone (DEX). Similarly, preincubation of testicular cells from untreated mice with DEX, corticosterone, or 11-dehydrocorticosterone reduced LH-stimulated testosterone release, as did the 11β-hydroxysteroid dehydrogenase inhibitors, glycyrrhetinic acid and carbenoxolone. The inhibitory actions of the steroids were mimicked by annexin 11–188 (ANXA11–188) (a stable annexin 1 analog). IL-1β produced a marked decrease in the response to LH, which was blocked by indomethacin, a nonselective cyclooxygenase inhibitor and an additive effect with DEX and ANXA11–188. These results confirm reports that glucocorticoids and IL-1β inhibit LH-stimulated testosterone release from mouse testicular cells. They also show, for the first time, that the effects of the steroids are mimicked by annexin 1 and that, in contrast to their mutually antagonistic effects in the neuroendocrine system, IL-1β and annexin 1 exert additive actions in the testis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Mulla, A. and Buckingham, J. C. (1999). Baillieres Best Pract. Res. Clin. Endocrinol. Metab. 13, 503–521.

    Article  PubMed  CAS  Google Scholar 

  2. Turnbull, A. V. and Rivier, C. L. (1999). Physiol. Rev. 79, 1–71.

    PubMed  CAS  Google Scholar 

  3. Luton, J. P., Thieblot, P., Valcke, J. C., Mahoudeau, J. A., and Bricaire, H. (1977). J. Clin. Endocrinol. Metab. 45, 488–495.

    PubMed  CAS  Google Scholar 

  4. Cumming, D. C., Quigley, M. E., and Yen, S. S. (1983). J. Clin. Endocrinol. Metab. 57, 671–673.

    PubMed  CAS  Google Scholar 

  5. Cunningham, G. R., Caperton, E. M. Jr., and Goldzieher, J. W. (1975). J. Clin. Endocrinol. Metab. 40, 265–267.

    PubMed  CAS  Google Scholar 

  6. Dubey, A. K. and Plant, T. M. (1985). Biol. Reprod. 33, 423–431.

    Article  PubMed  CAS  Google Scholar 

  7. Stalker, A., Hermo, L., and Antakly, T. (1989). Am. J. Anat. 186, 369–377.

    Article  PubMed  CAS  Google Scholar 

  8. Orr, T. E. and Mann, D. R. (1992). Horin. Behav. 26, 350–363.

    Article  CAS  Google Scholar 

  9. Monder, C. and White, P. C. (1993). Vitam. Horm. 47, 187–271.

    PubMed  CAS  Google Scholar 

  10. Seckl, J. R. (1993). Eur. J. Clin. Invest. 23, 589–601.

    PubMed  CAS  Google Scholar 

  11. Lakshmi, V. and Monder, C. (1985). Endocrinology 116, 552–560.

    PubMed  CAS  Google Scholar 

  12. Agarwal, A. K., Monder, C., Eckstein, B., and White, P. C. (1989). J. Biol. Chem. 264, 18,939–18,943.

    CAS  Google Scholar 

  13. Brown, R. W., Chapman, K. E., Edwards, C. R., and Seckl, J. R. (1993). Endocrinology 132, 2614–2621.

    Article  PubMed  CAS  Google Scholar 

  14. Albiston, A. L., Obeyesekere, V. R., Smith, R. E., and Krozowski, Z. S. (1994). Mol. Cell. Endocrinol. 105, R11-R17.

    Article  PubMed  CAS  Google Scholar 

  15. Gao, H. B., Shan, L. X., Monder, C., and Hardy, M. P. (1996). Endocrinology 137, 1714–1718.

    Article  PubMed  CAS  Google Scholar 

  16. Gao, H. B., Ge, R. S., Lakshmi, V., Marandici, A., and Hardy, M. P. (1997). Endocrinology 138, 156–161.

    Article  PubMed  CAS  Google Scholar 

  17. Leckie, C. M., Welberg, L. A., and Seckl, J. R. (1998). J. Endocrinol. 159, 233–238.

    Article  PubMed  CAS  Google Scholar 

  18. Condon, J., Ricketts, M. L., Whorwood, C. B., and Stewart, P. M. (1997). Mol. Cell. Endocrinol. 127, 121–128.

    Article  PubMed  CAS  Google Scholar 

  19. Welsh, T. H. Jr., Bambino, T. H., and Hsueh, A. J. (1982). Biol. Reprod. 27, 1138–1146.

    Article  PubMed  CAS  Google Scholar 

  20. Hales, D. B. and Payne, A. H. (1989). Endocrinology 124, 2099–2104.

    PubMed  CAS  Google Scholar 

  21. Agular, B. M. and Vind, C. (1995). J. Steroid Biochem. Mol. Biol. 54, 75–81.

    Article  PubMed  CAS  Google Scholar 

  22. Buckingham, J. C. and Flower, R. J. (1997). Mol. Med. Today 3, 296–302.

    Article  PubMed  CAS  Google Scholar 

  23. Philip, J. G., John, C. D., Cover, P. O., Morris, J. F., Christian, H. C., Flower, R. J., and Buckingham, J. C. (2001). Br. J. Pharmacol. 134, 887–895.

    Article  PubMed  CAS  Google Scholar 

  24. Taylor, A. D., Loxley, H. D., Flower, R. J., and Buckingham, J. C. (1995). Neuroendocrinology 62, 19–31.

    PubMed  CAS  Google Scholar 

  25. Taylor, A. D., Christian, H. C., Morris, J. F., Flower, R. J., and Buckingham, J. C. (1997). Endocrinology 138, 2909–2918.

    Article  PubMed  CAS  Google Scholar 

  26. Cirino, G., Peers, S. H., Flower, R. J., Browning, J. L., and Pepinsky, R. B. (1989). Proc. Natl. Acad. Sci. USA 86, 3428–3432.

    Article  PubMed  CAS  Google Scholar 

  27. Perretti, M. and Flower, R. J. (1993). J. Immunol. 150, 992–999.

    PubMed  CAS  Google Scholar 

  28. Perretti, M., Ahluwalia, A., Harris, J. G., Goulding, N. J., and Flower, R. J. (1993). J. Immunol. 151, 4306–4314.

    PubMed  CAS  Google Scholar 

  29. Carey, F., Forder, R., Edge, M. D., Greene, A. R., Horan, M. A., Strijbos, P. J., and Rothwell, N. J. (1990). Am. J. Physiol. 259, R266-R269.

    PubMed  CAS  Google Scholar 

  30. Violette, S. M., King, I., Browning, J. L., Pepinsky, R. B., Wallner, B. P., and Sartorelli, A. C. (1990). J. Cell. Physiol. 142, 70–77.

    Article  PubMed  CAS  Google Scholar 

  31. Croxtall, J. D. and Flower, R. J. (1992). Proc. Natl. Acad. Sci. USA 89, 3571–3575.

    Article  PubMed  CAS  Google Scholar 

  32. Buckingham, J. C. (1996). Br. J. Pharmacol. 118, 1–19.

    PubMed  CAS  Google Scholar 

  33. Ahluwalia, A., Buckingham, J. C., Croxtall, J. D., Flower, R. J., Goulding, N. J., and Perretti, M. (1996). In: The annexins. Seaton, B. A. (ed.). R. Landes and Co: Austin, TX.

    Google Scholar 

  34. Hales, D. B. (1992). Endocrinology 131, 2165–2172.

    Article  PubMed  CAS  Google Scholar 

  35. Calkins, J. H., Sigel, M. M., Nankin, H. R., and Lin, T. (1988). Endocrinology 123, 1605–1610.

    PubMed  CAS  Google Scholar 

  36. Fauser, B. C., Galway, A. B., and Hsueh, A. J. (1989). Acta Endocrinol. (Copenh.) 120, 401–408.

    CAS  Google Scholar 

  37. Huhtaniemi, I. and Toppari, J. (1995). Adv. Exp. Med. Biol. 377, 33–54.

    PubMed  CAS  Google Scholar 

  38. Jonsson, C. K., Setchell, B. P., Martinelle, N., Svechnikov, K., and Soder, O. (2001). Cytokine 14, 283–288.

    Article  PubMed  CAS  Google Scholar 

  39. Miller, S. C., Bowman, B. M., and Rowland, H. G. (1983). Am. J. Anat. 168, 1–13.

    Article  PubMed  CAS  Google Scholar 

  40. Taylor, A. D., Cowell, A. M., Flower, J., and Buckingham, J. C. (1993). Neuroendocrinology 58, 430–439.

    PubMed  CAS  Google Scholar 

  41. Pepinsky, R. B., Sinclair, L. K., Chow, E. P., and O’Brine-Greco, B. (1989). Biochem. J. 263, 97–103.

    PubMed  CAS  Google Scholar 

  42. Smith, T., Flower, R. J., and Buckingham, J. C. (1993). Mol. Neuropharmacol. 3, 45–55.

    CAS  Google Scholar 

  43. Bambino, T. H. and Hsueh, A. J. (1981). Endocrinology 108, 2142–2148.

    PubMed  CAS  Google Scholar 

  44. Hales, D. B., Sha, L. L., and Payne, A. H. (1987). J. Biol. Chem. 262, 11,200–11,206.

    CAS  Google Scholar 

  45. Bernier, M., Gibb, W., Collu, R., and Ducharme, J. R. (1984). Can. J. Physiol. Pharmacol. 62, 1166–1169.

    PubMed  CAS  Google Scholar 

  46. Gao, H. B., Tong, M. H., Hu, Y. Q., Guo, Q. S., Ge, R., and Hardy, M. P. (2002). Endocrinology 143, 130–138.

    Article  PubMed  CAS  Google Scholar 

  47. Loxley, H. D., Cowell, A. M., Flower, R. J., and Buckingham, J. C. (1993). Neuroendocrinology 57, 801–814.

    PubMed  CAS  Google Scholar 

  48. Jamieson, P. M., Chapman, K. E., Edwards, C. R., and Seckl, J. R. (1995). Endocrinology 136, 4754–4761.

    Article  PubMed  CAS  Google Scholar 

  49. Jamieson, P. M., Walker, B. R., Chapman, K. E., Andrew, R., Rossiter, S., and Seckl, J. R. (2000). J. Endocrinol. 165, 685–692.

    Article  PubMed  CAS  Google Scholar 

  50. Rajan, V., Chapman, K. E., Lyons, V., Jamieson, P., Mullins, J. J., Edwards, C. R., and Seckl, J. R. (1995). J. Steroid Biochem. Mol. Biol. 52, 141–147.

    Article  PubMed  CAS  Google Scholar 

  51. Kotelevtsev, Y., Holmes, M. C., Burchell, A., et al. (1997). Proc. Natl. Acad. Sci. USA 94, 14,924–14,929.

    Article  CAS  Google Scholar 

  52. Soro, A., Panarelli, M., Holloway, C. D., Fraser, R., and Kenyon, C. J. (1997). Steroids 62, 388–394.

    Article  PubMed  CAS  Google Scholar 

  53. Latif, S. A., Conca, T. J., and Morris, D. J. (1990). Steroids 55, 52–58.

    Article  PubMed  CAS  Google Scholar 

  54. O’Bryan, M. K., Schlatt, S., Phillips, D. J., de Kretser, D. M., and Hedger, M. P. (2000). Endocrinology 141, 238–246.

    Article  PubMed  CAS  Google Scholar 

  55. Emilie, D., Crevon, M. C., Auffredou, M. T., and Galanaud, P. (1988). Eur. J. Immunol. 18, 2043–2047.

    Article  PubMed  CAS  Google Scholar 

  56. Wu, C. Y., Sarfati, M., Heusser, C., Fournier, S., Rubio-Trujillo, M., Peleman, R., and Delespesse, G. (1991). J. Clin. Invest. 87, 870–877.

    Article  PubMed  CAS  Google Scholar 

  57. Akahoshi, T., Oppenheim, J. J., and Matsushima, K. (1988). J. Exp. Med. 167, 924–936.

    Article  PubMed  CAS  Google Scholar 

  58. Escher, G., Galli, I., Vishwanath, B. S., Frey, B. M., and Frey, F. J. (1997). J. Exp. Med. 186, 189–198.

    Article  PubMed  CAS  Google Scholar 

  59. Wong, M. L., Rettori, V., al-Shekhlee, A., Bongiorno, P. B., Canteros, G., McCann, S. M., Gold, P. W., and Licinio, J. (1996). Nat. Med. 2, 581–584.

    Article  PubMed  CAS  Google Scholar 

  60. Moore, C. and Moger, W. H. (1991). J. Endocrinol. 129, 381–390.

    PubMed  CAS  Google Scholar 

  61. Lin, T., Wang, T. L., Nagpal, M. L., Calkins, J. H., Chang, W. W., and Chi, R. (1991). Endocrinology 129, 1305–1311.

    Article  PubMed  CAS  Google Scholar 

  62. Lin, T. (1985). Life Sci. 36, 1255–1264.

    Article  PubMed  CAS  Google Scholar 

  63. Vane, J. R. and Botting, R. M. (1998). Inflamm. Res. 47(Suppl. 2), S78-S87.

    Article  PubMed  CAS  Google Scholar 

  64. Janszen, F. H., Cooke, B. A., van Driel, M. J., and van der Molen, H. J. (1976). J. Endocrinol. 70, 345–359.

    Article  PubMed  CAS  Google Scholar 

  65. Collins, W. P., Mansfield, M. D., Alladina, N. S., and Sommerville, I. F. (1972). J. Steroid Biochem. 3, 333–348.

    Article  PubMed  CAS  Google Scholar 

  66. Bradford, M. M. (1976). Anal. Biochem. 72, 248–254.

    Article  PubMed  CAS  Google Scholar 

  67. Lim, L. H., Solito, E., Russo-Marie, F., Flower, R. J., and Perretti, M. (1998). Proc. Natl. Acad. Sci. USA 95, 14,535–14,539.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Medicine, Department of Neuroendocrinology, Division of Neuroscience and Psychological Medicine, Imperial College of Science Technology and Medicine, Commonwealth Building, Hammersmith Hospital, Du Cane Road, W12 0NN, London

    Patricia O. Cover, Frederick Baanah-Jones, Christopher D. John & Julia C. Buckingham

Authors
  1. Patricia O. Cover
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Frederick Baanah-Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christopher D. John
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Julia C. Buckingham
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Patricia O. Cover.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cover, P.O., Baanah-Jones, F., John, C.D. et al. Annexin 1 (lipocortin 1) mimics inhibitory effects of glucocorticoids on testosterone secretion and enhances effects of interleukin-1β. Endocr 18, 33–39 (2002). https://doi.org/10.1385/ENDO:18:1:33

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ENDO:18:1:33

Key Words

Search

Navigation