Abstract
Initiation and maintenance of labor in humans is associated with an increase in prostaglandin synthesis by intrauterine tissues. The objective of the present study was to characterize the distribution of membrane-bound PGES (mPGES) protein and mPGES mRNA in human placenta, fetal membranes, and decidua at term and to determine whether any changes occurred with labor. Immunoreactive mPGES was found to be highly concentrated in amnion epithelial cells and the chorion laeve trophoblasts, with lower levels in the mesenchymal layers. The enzyme was at very low levels or undetectable in the decidual tissue. Much lower levels of mPGES protein and mRNA were found in placenta than in fetal membranes. mPGES was associated with the syncytiotrophoblast and in cells surrounding blood vessels. The expression of mPGES mRNA did not change with labor in full membranes or placenta, but Western analysis showed an increase in mPGES protein in chorion laeve and a decrease in mPGES protein in placenta during labor, with no change in the amnion. The differences in expression found among placenta, chorion, and amnion before and after labor would indicate that this enzyme is differentially regulated in these tissues at this time.
Similar content being viewed by others
References
Challis, J. R. G., Lye, S. J., and Gibb, W. (1997). Ann. NY Acad. Sci. 828, 254–267.
Gibb, W. (1998). Ann. Med. 30, 235–241.
Hansen, W. R., Keelan, J. A., Skinner, S. J. M., and Mitchell, M. D. (1999). Prostaglandins Lipid Mediat. 57, 243–257.
Jakobsson, P., Thoren, S., Morgenstern, R., and Samuelsson, B. (1999). Proc. Natl. Acad. Sci. USA 96, 7220–7225.
Watanabe, K., Kurihara, K., and Suzuki, T. (1999). Biochim. Biophys. Acta 143, 406–414.
Moonen, P., Buytenhek, M., and Nugteren, D. H. (1982). Methods Enzymol. 86, 84–90.
Tanioka, T., Nakatani, Y., Semmyo, N., Murakami, M., and Kudo, I. (2000). J. Biol. Chem. 275, 32775–32782.
Murakami, M., Naraba, H., Tanioka, T., et al. (2000). J. Biol. Chem. 275, 32783–32792.
Gibb, W., Matthews, S. G., and Challis, J. R. G. (1996). Biol. Reprod. 5, 654–659.
Martin, R. L., Whittle, W. L., Holloway, A. C., et al. (2002). Biol. Reprod. 67, 868–873.
Olson, D. M., Skinner, W., Challis, J. R. G. (1983). J. Clin. Endocrinol. Metab. 57, 694–697.
Teixeira, F., Zakar, J., Hirst, J., Guo, F., et al. (1994). J. Clin. Endocrinol. Metab. 78, 1396–1402.
Hirst, J. J., Teixeira, F. J., Zakar, T., and Olson, D. M. (1996). J. Clin. Endocrinol. Metab. 80, 517–523.
Mijovic, J. C., Zakar, T., Nairn, T. K., and Olson, D. M. (1997). Am. J. Physiol. 172, 8832–8840.
Dudley, D., Trautman, M., and Mitchell, M. (1993). J. Clin. Endocrinol. Metab. 76, 406–410.
Gibb, W. and Sun, M. (1996). J. Endocrinology 150, 497–503.
McLaren, W. J., Young, I. R., and Rice, G. E. (2000). J. Reprod. Fertil. 120, 33–39.
Mendez, M. and La Pointe, M. C. (2002). Hypertension 39, 382–388.
Thoren, S. and Jakobsson, P. (2000) Eur. J. Biochem. 267, 6428–6434.
Giannoulias, D., Alfaidy, N., Holloway, A. C., et al. (2002). J. Clin. Endocrinol. Metab. 87, 5274–5282.
Tanaka, Y., Ward, S., and Smith, W. L. (1987). J. Biol. Chem. 262, 1374–1381.
Solar, M., Camacho, M., Escudero, J., Inguez, M., and Vila, L. (2000). Circ. Res. 87, 504–507.
Clark, K. E., Farley, D. B., Van Orden, D. E., and Brody, M. J. (1977). Am. J. Obstet. Gynecol. 127, 455–461.
Ulug, U., Goldman, S., Ben-Shlomo, I., and Shalev, E. (2001). Mol. Hum. Reprod. 7, 1187–1193.
Alfaidy, N., Xiong, Z. G., Myatt, L., Lye, S. J., MacDonald, J. F., and Challis, J. R. (2001). J. Clin. Endocrinol. Metab. 86, 5585–5592.
Riley, S. C., Boshier, D. P., Luu-The, V., Labrie, F., and Challis, J. R. (1992). J. Reprod. Fertil. 96, 127–134.
Bradford, M. M. (1976). Anal. Biochem. 72, 248–254.
Forsberg, L., Leeb, L., Thoren, S., Morgenstern, R., and Jacobsson, P. (2000). FEBS Lett. 471, 78–82.
Altschul, F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990). J. Mol. Biol, 215, 403–410.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Alfaidy, N., Sun, M., Challis, J.R.G. et al. Expression of membrane prostaglandin E synthase in human placenta and fetal membranes and effect of labor. Endocr 20, 219–225 (2003). https://doi.org/10.1385/ENDO:20:3:219
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/ENDO:20:3:219