Abstract
Objective: Cytochrome P450 (CYP) 2J2 plays an important role in the biosynthesis of the biologically active cis-epoxyeicosatrienoic acids. An allelic variant named CYP2J2*6, which encodes an enzyme that is almost inactive in the metabolism of arachidonic acid, has recently been described. We investigated the frequency of the CYP2J2*6 variant in a Caucasian population and the relationship between this polymorphism and the development of micro- and macrovascular complications and hypertension in patients with type 1 or type 2 diabetes mellitus.
Methods: Genomic DNA was extracted from peripheral blood cells and the fragment containing the A/T single nucleotide polymorphism at position 25 661 in exon 8 of the CYP2J2 gene was amplified. The 532 bp amplified product was subsequently digested with Tsp509I and analyzed on 12% polyacrylamide gel electrophoresis.
Results: In the whole population, the frequency of the CYP2J2*6 allele was 0.0064 and the frequency of the CYP2J2*1 allele was 0.9936. Genotype distribution did not show significant differences between controls and patients with type 1 or type 2 diabetes. No homozygotes for CYP2J2*6 allele were found. No association was found between this allele and complications or hypertension in either type of diabetes.
Conclusion: The CYP2J2*6 allele is rare in the Caucasian population, and no association is inferred between this allelic variant and diabetic complications.
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Acknowledgements
This study was supported by grants from Istituto Superiore di Sanità. Programmi di Ricerca Finalizzata 1998. Convenzione N. 93-99/U/T4.
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Pucci, L., Lucchesi, D., Chirulli, V. et al. Cytochrome P450 2J2 Polymorphism in Healthy Caucasians and those with Diabetes Mellitus. Am J Pharmacogenomics 3, 355–358 (2003). https://doi.org/10.2165/00129785-200303050-00006
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DOI: https://doi.org/10.2165/00129785-200303050-00006