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Plant Endosomes

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Marisa S. Otegui

    1. Department of Botany and Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison, Madison, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2177)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis

    • Akira Yoshinari, Junpei Takano
    Pages 1-13

  3. Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles

    • Mengqian Luo, Ying Zhu, Zhiqi Liu, Liwen Jiang
    Pages 15-21

  4. Analysis of Endoplasmic Reticulum–Endosome Association Using Live-Cell Imaging in Plant Cells

    • Giovanni Stefano, Federica Brandizzi
    Pages 23-33

  5. Degradation of Abscisic Acid Receptors Through the Endosomal Pathway

    • Borja Belda-Palazón, Pedro L. Rodriguez
    Pages 35-48

  6. Biochemical and Imaging Analysis of ALIX Function in Endosomal Trafficking of Arabidopsis Protein Cargoes

    • Marta García-León, Vicente Rubio
    Pages 49-58

  7. Correlative Light and Electron Microscopy Imaging of the Plant trans-Golgi Network

    • Pengfei Wang, Byung-Ho Kang
    Pages 59-67

  8. Imaging Plant Cells by High-Pressure Freezing and Serial block-face scanning electron microscopy

    • Kirk Czymmek, Abhilash Sawant, Kaija Goodman, Janice Pennington, Pal Pedersen, Mrinalini Hoon et al.
    Pages 69-81

  9. Functional Analysis of Plant FYVE Domain Proteins in Endosomal Trafficking

    • Wenjin Shen, Juan Wei, Caiji Gao
    Pages 83-94

  10. Assessing Extrinsic Membrane Protein Dependency to PI4P Using a Plasma Membrane to Endosome Relocalization Transient Assay in Nicotiana benthamiana

    • Mehdi Doumane, Marie-Cécile Caillaud
    Pages 95-108

  11. Subcellular Localization of PI3P in Arabidopsis

    • Han Nim Lee, Hyera Jung, Taijoon Chung
    Pages 109-118

  12. Immunopurification of Intact Endosomal Compartments for Lipid Analyses in Arabidopsis

    • Yoko Ito, Magali Grison, Nicolas Esnay, Laetitia Fouillen, Yohann Boutté
    Pages 119-141

  13. Cell-Free Protein Translation System for Expression of Lipid-Binding Proteins Tagged with Small epitopes and Their Use in Protein–Lipid Overlay Assays

    • Julio Paez-Valencia, Marisa S. Otegui
    Pages 143-152

  14. Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants

    • Guangxi Ren, Michel Ruiz Rosquete, Angelo G. Peralta, Sivakumar Pattathil, Michael G. Hahn, Thomas Wilkop et al.
    Pages 153-167

  15. Detection of Phosphorylation on Immunoprecipitates from Total Protein Extracts of Arabidopsis thaliana Seedlings

    • Karin Vogel, Erika Isono
    Pages 169-182

  16. Purification and Interaction Analysis of a Plant-Specific RAB5 Effector by In Vitro Pull-Down Assay

    • Emi Ito, Seung-won Choi, Takashi Ueda
    Pages 183-197

  17. Back Matter

    Pages 199-200
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Keywords

About this book

This second edition details techniques for the study of cargo trafficking through endosomes. New and updated chapters guide readers through methods and protocols on the structural aspects of plant endosomes, combined biochemical, omics, imaging approaches to study the dynamics and contents of endosomal compartments. Additional chapters are dedicated to the analysis of lipids on endosomes and the identification and analysis of lipid binding proteins and lipid-binding domains relevant for the study of plant endosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Plant Endosomes: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Department of Botany and Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison, Madison, USA

    Marisa S. Otegui

Bibliographic Information

  • Book Title: Plant Endosomes

  • Book Subtitle: Methods and Protocols

  • Editors: Marisa S. Otegui

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0767-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0766-4Published: 07 July 2020

  • Softcover ISBN: 978-1-0716-0769-5Published: 07 July 2021

  • eBook ISBN: 978-1-0716-0767-1Published: 06 July 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 200

  • Number of Illustrations: 10 b/w illustrations, 31 illustrations in colour

  • Topics: Plant Sciences, Cell Biology

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