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Mouse Genetics

Methods and Protocols

  • Book
  • © 2021
  • Latest edition

Overview

Editors:
  1. Shree Ram Singh

    1. Basic Research Laboratory, National Cancer Institute, Frederick, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Robert M. Hoffman

    1. Department of Surgery, University of California, AntiCancer, Inc., San Diego, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Amit Singh

    1. Department of Biology, University of Dayton, Dayton, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2224)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Generation of Mouse Model (KI and CKO) via Easi-CRISPR

    • Dorjee T. N. Shola, Chingwen Yang, Chiayun Han, Rada Norinsky, Ruben D. Peraza
    Pages 1-27

  3. Analysis of Gene Expression Using lacZ Reporter Mouse Lines

    • Michael Simon Krämer, Robert Feil, Hannes Schmidt
    Pages 29-45

  4. Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice

    • Benedetta Ruzzenente, Metodi D. Metodiev
    Pages 47-60

  5. Mouse Models for Studying Hippocampal Adult Neural Stem Cell Biology

    • Fatih Semerci, Luke Parkitny, Mirjana Maletic-Savatic
    Pages 61-74

  6. Retina as a Model to Study In Vivo Transmission of α-Synuclein in the A53T Mouse Model of Parkinson’s Disease

    • Najiba Mammadova, Thierry Baron, Jérémy Verchère, Justin J. Greenlee, M. Heather West Greenlee
    Pages 75-85

  7. Promoting Pro-Endocrine Differentiation and Graft Maturation Following Surgical Resection of the Mouse Pancreas

    • Mugdha V. Joglekar, Rohan R. Patil, Sarang N. Satoor, Wilson K. M. Wong, Mahesh S. Karandikar, Anandwardhan A. Hardikar
    Pages 87-98

  8. Color-Coded Imaging of Cancer and Stromal-Cell Interaction in the Pancreatic-Cancer Tumor Microenvironment (TME)

    • Atsushi Suetsugu, Robert M. Hoffman
    Pages 99-111

  9. Generating Ins2+/−/miR-133aTg Mice to Model miRNA-Driven Cardioprotection of Human Diabetic Heart

    • Hamid R. Shahshahan, Tyler N. Kambis, Sumit Kar, Santosh K. Yadav, Paras K. Mishra
    Pages 113-121

  10. Generating a Podocyte-Specific Neonatal F Receptor (FcRn) Knockout Mouse

    • Judith Blaine
    Pages 123-132

  11. Mouse Models of Colitis-Associated Colon Cancer

    • Santhakumar Manicassamy, Puttur D. Prasad, Daniel Swafford
    Pages 133-146

  12. Generation of Colon Cancer Model Based on Colonoscopy Injection

    • Suhwan Chang
    Pages 147-152

  13. Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse

    • Jeffrey Barminko, Andrei M. Vacaru, Margaret H. Baron
    Pages 153-182

  14. Gene Inactivation in Adult Long-Term Hematopoietic Stem Cells Using Inducible Mouse Models

    • Kristbjorn Orri Gudmundsson
    Pages 183-193

  15. Hematological Humanization of Immune-Deficient Mice

    • Marina Gergues, Seda Ayer, Sara Morelli, Steven J. Greco, Pranela Rameshwar
    Pages 195-202

  16. Antisense Oligonucleotide Treatment in a Humanized Mouse Model of Duchenne Muscular Dystrophy and Highly Sensitive Detection of Dystrophin Using Western Blotting

    • Rika Maruyama, Toshifumi Yokota
    Pages 203-214

  17. Back Matter

    Pages 215-217
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Keywords

About this book

This fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases.

Editors and Affiliations

  • Basic Research Laboratory, National Cancer Institute, Frederick, USA

    Shree Ram Singh

  • Department of Surgery, University of California, AntiCancer, Inc., San Diego, USA

    Robert M. Hoffman

  • Department of Biology, University of Dayton, Dayton, USA

    Amit Singh

Bibliographic Information

  • Book Title: Mouse Genetics

  • Book Subtitle: Methods and Protocols

  • Editors: Shree Ram Singh, Robert M. Hoffman, Amit Singh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1008-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1007-7Published: 20 February 2021

  • Softcover ISBN: 978-1-0716-1010-7Published: 20 February 2022

  • eBook ISBN: 978-1-0716-1008-4Published: 19 February 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 217

  • Number of Illustrations: 6 b/w illustrations, 41 illustrations in colour

  • Topics: Biological Techniques, Biomedicine, general, Zoology

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