Skip to main content
SpringerLink
Log in

Plant Phosphoproteomics

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Xu Na Wu

    1. State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, China

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2358)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Phosphorylation Site Motifs in Plant Protein Kinases and Their Substrates

    • Lin Xi, Zhaoxia Zhang, Sandra Herold, Sarah Kassem, Xu Na Wu, Waltraud X. Schulze
    Pages 1-16

  3. Protein Phosphorylation Response to Abiotic Stress in Plants

    • Rebecca Njeri Damaris, Pingfang Yang
    Pages 17-43

  4. Protein Phosphorylation in Plant Cell Signaling

    • Ping Li, Junzhong Liu
    Pages 45-71

  5. Phosphoproteomics Profiling of Receptor Kinase Mutants

    • Dandan Lu, Ting Gao, Lin Xi, Leonard Krall, Xu Na Wu
    Pages 73-82

  6. Phosphoproteomic Analysis of Soybean Roots Under Salinity by Using the iTRAQ Labeling Approach

    • Yuchen Qian, Jia Xu, Erxu Pi
    Pages 83-91

  7. Universal Sample Preparation Workflow for Plant Phosphoproteomic Profiling

    • Chuan-Chih Hsu, Justine V. Arrington, W. Andy Tao
    Pages 93-103

  8. Mapping Plant Phosphoproteome with Improved Tandem MOAC and Label-Free Quantification

    • Yanmei Chen, Xinlin Liang
    Pages 105-112

  9. SILIA-Based 4C Quantitative PTM Proteomics

    • Emily Oi Ying Wong, Ning Li
    Pages 113-135

  10. Phosphoproteomics Analysis of Plant Root Tissue

    • Zhe Zhu, Shubo Yang, Shalan Li, Xiaolin Yang, Leonard Krall
    Pages 137-144

  11. Plant Phosphopeptides Enrichment by Immobilized Metal Ion Affinity Chromatography

    • Xiahe Huang, Yuanya Zhang, Haitao Ge, Dandan Lu, Yingchun Wang
    Pages 145-157

  12. Two-Dimensional Gel Electrophoresis and Pro-Q Diamond Phosphoprotein Stain-Based Plant Phosphoproteomics

    • Yuan Li, Dongtao Ren
    Pages 159-168

  13. 2-D DIGE Combined with Pro-Q Diamond Staining for the Identification of Protein Phosphorylation for Chlamydomonas reinhardtii : A Successful Approach

    • Li Li
    Pages 169-177

  14. Plant Phosphopeptide Identification and Label-Free Quantification by MaxQuant and Proteome Discoverer Software

    • Shalan Li, Haitao Zan, Zhe Zhu, Dandan Lu, Leonard Krall
    Pages 179-187

  15. PhosPhAt 4.0: An Updated Arabidopsis Database for Searching Phosphorylation Sites and Kinase-Target Interactions

    • Lin Xi, Zhaoxia Zhang, Waltraud X. Schulze
    Pages 189-202

  16. Computational Phosphorylation Network Reconstruction: An Update on Methods and Resources

    • Min Zhang, Guangyou Duan
    Pages 203-219

  17. The Application of an R Language-Based Platform cRacker for Phosphoproteomics Data Analysis

    • Mingjie He, Zhi Li
    Pages 221-228

  18. Kinase Activity Assay Using Unspecific Substrate or Specific Synthetic Peptides

    • Jiahui Wang, Xiaolin Yang, Lin Xi, Xu Na Wu
    Pages 229-237

  19. Correction to: Plant Phosphopeptide Identification and Label-Free Quantification by MaxQuant and Proteome Discoverer Software

    • Shalan Li, Haitao Zan, Zhe Zhu, Dandan Lu, Leonard Krall
    Pages C1-C1

  20. Back Matter

    Pages 239-241
    Download chapter PDF
Back to top

Keywords

About this book

This detailed protocol book provides phosphoproteomics techniques currently developed for use in plants, as well as offering a variety of methodologies to analyze different types of plant phosphoproteomic data. Suitable protocols for every stage of the experiment, from protein isolation and phosphopeptides enrichment to large-scale data analysis, have been considered and explored. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Phosphoproteomics: Methods and Protocols serves as an ideal guide for researchers examining phosphoproteomics in the field of plant sciences.

Editors and Affiliations

  • State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, China

    Xu Na Wu

Bibliographic Information

  • Book Title: Plant Phosphoproteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Xu Na Wu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1625-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1624-6Published: 17 July 2021

  • Softcover ISBN: 978-1-0716-1627-7Published: 18 July 2022

  • eBook ISBN: 978-1-0716-1625-3Published: 16 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 241

  • Number of Illustrations: 3 b/w illustrations, 26 illustrations in colour

  • Topics: Plant Sciences, Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation