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Estrogen Receptors

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Kathleen M. Eyster

    1. Sanford School of Medicine, University of South Dakota, Vermillion, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2418)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Antibody Validation for Estrogen Receptor Beta

    • Madeleine Birgersson, Borbala Katona, Cecilia Lindskog, Fredrik Pontén, Cecilia Williams
    Pages 1-23

  3. Immunoblot Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 /GPER1 Activation

    • Sara Clark, Kevin Pollard, Jennifer Rainville, Nandini Vasudevan
    Pages 25-39

  4. Immunoprecipitation Analyses of Estrogen Receptor α Phosphorylated at Serine 216 in the Mouse Liver

    • MyeongJin Yi, Sawako Shindo, Masahiko Negishi
    Pages 41-51

  5. Immunofluorescent Verification of Silencing Estrogen Receptor α with siRNA in the Intact Rodent Brain

    • Ana C. Ribeiro, Mihaela A. Stavarache, Sergei Musatov, Donald W. Pfaff
    Pages 53-61

  6. Detection and Functional Analysis of Estrogen Receptor α Phosphorylated at Serine 216 in Mouse Neutrophils

    • Sawako Shindo, Rick Moore, MyeongJin Yi, Masahiko Negishi
    Pages 63-75

  7. Quantification of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate in Response to Estrogen

    • Thomas W. Bonagura, Jeffery S. Babischkin, Gerald J. Pepe, Eugene D. Albrecht
    Pages 77-93

  8. In Situ Hybridization of Estrogen Receptors α and β in the Human Testis

    • Katja Hartmann, Daniela Fietz
    Pages 95-112

  9. Extraction of RNA and Analysis of Estrogen-Responsive Genes by RT-qPCR

    • Michelle L. Booze, Kathleen M. Eyster
    Pages 113-127

  10. PET Imaging of Estrogen Receptors Using 18F-Based Radioligands

    • Manoj Kumar, Kelley Salem, Justin J. Jeffery, Amy M. Fowler
    Pages 129-151

  11. The Use of ERE-Luc Reporter Mice to Monitor Estrogen Receptor Transcriptional Activity in a Spatio-Temporal Dimension

    • Sara Della Torre, Elisabetta Vegeto, Paolo Ciana
    Pages 153-172

  12. Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model

    • Daniel A. Gorelick, Caroline Lucia, Ruixin Hao, Silvia Karim, Maria Bondesson
    Pages 173-185

  13. Assessing Estrogenic Activity of Classical Estrogen Receptor -Binding Compounds

    • Richard A. Pepermans, Eric R. Prossnitz
    Pages 187-201

  14. An Optimized ChIP-Seq Protocol to Determine Chromatin Binding of Estrogen Receptor Beta

    • Rajitha Indukuri, Anastasios Damdimopoulos, Cecilia Williams
    Pages 203-221

  15. Polysome Fractionation for Transcriptome-Wide Studies of mRNA Translation

    • Johannes Ristau, Kathleen Watt, Christian Oertlin, Ola Larsson
    Pages 223-241

  16. Anota2seq Analysis for Transcriptome-Wide Studies of mRNA Translation

    • Christian Oertlin, Kathleen Watt, Johannes Ristau, Ola Larsson
    Pages 243-268

  17. Whole-Genome Genotyping Using DNA Microarrays for Population Genetics

    • Austin J. Van Asselt, Erik A. Ehli
    Pages 269-287

  18. Proteomics Analysis of the Estrogen Effects in the Rat Uterus Using Gel-LC and Tandem Mass Spectrometry Approaches

    • Maria D. Paez, Eduardo A. Callegari
    Pages 289-311

  19. Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells

    • Amena Archer, Claudia Kutter, Cecilia Williams
    Pages 313-343

  20. Molecular Cloning and Purification of the Protein Lysine Methyltransferase SMYD2 and its Co-crystallization with a Target Peptide from Estrogen Receptor Alpha

    • Yingxue Zhang, Zhe Yang
    Pages 345-362
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Keywords

About this book

This fully updated collection includes chapters on a wide array of techniques that are vital for advancing our understanding of the physiological and pathological effects of estrogen, mediated by estrogen receptor α (ERα) and estrogen receptor β (ERβ) as well as the non-genomic membrane-bound G-protein coupled estrogen receptor (GPER/GPR30). The protocols range from standard methods and important laboratory workhorses such as immunoblot and RT-qPCR, to newer technologies such as PET imaging, reporter animal models, polysome fractionation, and many others, involving a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written for this highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative andpractical, Estrogen Receptors: Methods and Protocols, Second Edition serves as an ideal guide to researchers working to explore the vibrancy and excitement of the field of estrogen receptors and estrogen actions.

Editors and Affiliations

  • Sanford School of Medicine, University of South Dakota, Vermillion, USA

    Kathleen M. Eyster

Bibliographic Information

  • Book Title: Estrogen Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Kathleen M. Eyster

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1920-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1919-3Published: 05 February 2022

  • Softcover ISBN: 978-1-0716-1922-3Published: 05 February 2023

  • eBook ISBN: 978-1-0716-1920-9Published: 04 February 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 427

  • Number of Illustrations: 13 b/w illustrations, 68 illustrations in colour

  • Topics: Physiology

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