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Microcephaly

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Timothy Gershon

    1. Department of Pediatrics, Children’s Center for Neuroscience Research, Emory University School of Medicine, Atlanta, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2583)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
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  3. Analyses of Neural Progenitors In Situ

    1. Front Matter

      Pages 47-47
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    2. Processing Neonatal Mouse Brains for Immunohistochemical Analysis: As Required for Including Spindle Orientation Analysis and Analysis of DNA Damage and Apoptosis

      • Gabriela De la Cruz, Nana Nikolaishvili-Feinberg, Scott E. Williams, Timothy R. Gershon
      Pages 49-54

    3. Automated Immunofluorescence Staining for Analysis of DNA Damage and Apoptosis in Brain Sections

      • Gabriela De la Cruz, Nana Nikolaishvili-Feinberg, Timothy R. Gershon
      Pages 55-61

    4. Automated Immunofluorescence Staining for Analysis of Mitotic Stages and Division Orientation in Brain Sections

      • Gabriela De la Cruz, Nana Nikolaishvili Feinberg, Scott E. Williams
      Pages 63-79

  4. RNA-seq Analysis with Single-Cell Resolution (scRNA-seq)

    1. Front Matter

      Pages 81-81
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    2. scRNA-seq for Microcephaly Research [I]: Single-Cell Droplet Encapsulation, mRNA Capture, and cDNA Synthesis

      • Benjamin Babcock, Seth Weir
      Pages 83-97

    3. scRNA-seq for Microcephaly Research [II]: Preparation of Single-Cell cDNA Libraries

      • Benjamin Babcock, Seth Weir
      Pages 99-104

    4. scRNA-seq for Microcephaly Research [III]: Computational Analysis of scRNA-seq Data

      • Benjamin Babcock, Daniel Malawsky
      Pages 105-121

    5. scRNA-seq for Microcephaly Research [IV]: Dirichlet Regression for Single-Cell Population Differences

      • Daniel Malawsky, Timothy R. Gershon
      Pages 123-125

  5. MRI Analyses of the Mouse Brain

    1. Front Matter

      Pages 127-127
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    2. Mouse Brain MRI: Including In Vivo, Ex Vivo, and fcMRI for the Study of Microcephaly

      • Martin J. MacKinnon, Tzu-Wen W. Wang, Yen-Yu I. Shih
      Pages 129-148

    3. Whole-Body Mouse Fluxomic Analysis to Detect Metabolic Disruptions Associated with Microcephaly: Using 13C Isotopes

      • Andrey P. Tikunov
      Pages 149-156

  6. Behavioral Phenotyping in Neonatal Mice

    1. Front Matter

      Pages 157-157
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    2. Neonatal Behavioral Screen for Mouse Models of Neurodevelopmental Disorders

      • Kathryn M. Harper, Viktoriya D. Nikolova, Monika E. Conrad, Sheryl S. Moy
      Pages 159-173
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Keywords

About this book

This volume discusses the latest imaging and molecular techniques for studying microcephaly through neural progenitor proliferation, survival, and gene expression. All of the methods covered in this book use the mouse as a model system, and include cellular, metabolic, transcriptomic studies of neural progenitors, MR imaging studies of brain growth in 3-dimensions and developmentally-adapted studies of behavior in neonatal mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Microcephaly: Methods and Protocols is a valuable resource for all researchers who want to expand their knowledge and understanding of this important and developing field.

Editors and Affiliations

  • Department of Pediatrics, Children’s Center for Neuroscience Research, Emory University School of Medicine, Atlanta, USA

    Timothy Gershon

Bibliographic Information

  • Book Title: Microcephaly

  • Book Subtitle: Methods and Protocols

  • Editors: Timothy Gershon

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2752-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2751-8Published: 24 November 2022

  • Softcover ISBN: 978-1-0716-2754-9Published: 24 November 2023

  • eBook ISBN: 978-1-0716-2752-5Published: 23 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 176

  • Number of Illustrations: 12 b/w illustrations, 24 illustrations in colour

  • Topics: Neurosciences

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