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SILAC

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Jose L. Luque-Garcia

    1. Analytical Chemistry Department, Faculty of Chemical Sciences, Complutense University of Madrid, Madrid, Spain

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2603)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Dynamic SILAC to Determine Protein Turnover in Neurons and Glia

    • Aline R. Dörrbaum, Erin M. Schuman, Julian D. Langer
    Pages 1-17

  3. SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe

    • Dorothea Anrather, Silvia Bagelova Polakova, Lubos Cipak, Juraj Gregan
    Pages 19-29

  4. Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Quantitative Proteomics

    • Monika Kopeckova, Marek Link, Ivona Pavkova
    Pages 31-42

  5. pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles

    • So-Fong Cam Ngan, Neil E. McCarthy, Siu Kwan Sze
    Pages 43-58

  6. Quantification of Protein Palmitoylation by Cysteine-SILAC

    • Caiyun Fang, Xiaoqin Zhang, Haojie Lu
    Pages 59-69

  7. Comparative SUMO Proteome Analysis Using Stable Isotopic Labeling by Amino Acids (SILAC)

    • Georgia Chachami, Sina-Victoria Barysch
    Pages 71-86

  8. A Super-SILAC Approach for Profiling Histone Posttranslational Modifications

    • Roberta Noberini, Elisa Longhi, Tiziana Bonaldi
    Pages 87-102

  9. SILAC-Based Quantitative Phosphoproteomics in Yeast

    • María Luisa Hernáez, Concha Gil
    Pages 103-115

  10. Phosphotyrosine Profiling Using SILAC

    • Keshava K. Datta, Aditi Chatterjee, Harsha Gowda
    Pages 117-125

  11. Chemoproteomic Profiling of Geranyl Pyrophosphate-Binding Proteins

    • Rong Cai, Yinsheng Wang
    Pages 127-138

  12. Quantitative Analysis of the Endoplasmic Reticulum-Associated Proteins Using ER-Localizable Reactive Molecules

    • Tomonori Tamura, Itaru Hamachi
    Pages 139-150

  13. Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC

    • Alexis Gonneaud, Claude Asselin, Véronique Giroux, François-Michel Boisvert
    Pages 151-161

  14. Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons

    • Guoan Zhang, Katrin Deinhardt, Thomas A. Neubert
    Pages 163-171

  15. Heavy Methyl SILAC Metabolic Labeling of Human Cell Lines for High-Confidence Identification of R/K-Methylated Peptides by High-Resolution Mass Spectrometry

    • Enrico Massignani, Marianna Maniaci, Tiziana Bonaldi
    Pages 173-186

  16. SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

    • Alistair Langlands, Hamze Beati, H.- Arno J. Müller
    Pages 187-198

  17. Super-SILAC Quantitative Proteome Profiling of Zebrafish Larvae

    • Guillermo Aragoneses-Cazorla, Andres Machuca, M. Pilar Buendia-Nacarino, Daniela S. Anunciação, Estefania Garcia-Calvo, Jose L. Luque-Garcia
    Pages 199-207

  18. Use of Nuclear and Chromatin Enrichment Procedures for Quantitation of Yeast DNA Replication Proteins Using SILAC

    • Takashi Kubota
    Pages 209-218

  19. Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trapping for the Detection of Transient Protein Interactions

    • Jolene M. Duda, Stefani N. Thomas
    Pages 219-234

  20. Profiling of Secreted Proteins in Serum-Containing Media Using BONCAT and Pulsed SILAC

    • Jihye Shin, Cheolju Lee
    Pages 235-243
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Keywords

About this book

This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science.

Editors and Affiliations

  • Analytical Chemistry Department, Faculty of Chemical Sciences, Complutense University of Madrid, Madrid, Spain

    Jose L. Luque-Garcia

Bibliographic Information

  • Book Title: SILAC

  • Book Subtitle: Methods and Protocols

  • Editors: Jose L. Luque-Garcia

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2863-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2862-1Published: 13 November 2022

  • Softcover ISBN: 978-1-0716-2865-2Published: 14 November 2023

  • eBook ISBN: 978-1-0716-2863-8Published: 12 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 286

  • Number of Illustrations: 10 b/w illustrations, 31 illustrations in colour

  • Topics: Chemistry/Food Science, general, Biological Techniques

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