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Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence

  • Book
  • © 1993

Overview

Editors:
  1. Peter H. Bach

    1. University of East London, London, UK

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  2. C. Hugh Reynolds

    1. University of East London, London, UK

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  3. Jessica M. Clark

    1. University of East London, London, UK

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  4. John Mottley

    1. University of East London, London, UK

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  5. Phil L. Poole

    1. University of East London, London, UK

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Table of contents (25 chapters)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Microinjection: A Technique to Study Inhibition of HIV-1 Replication Mediated by Antisense RNA and Parvovirus Genes

    • Georg Sczakiel, Ingo Oelze, Karola Rittner
    Pages 1-10

  3. How to Make Glass Microtools for the Injection of Isolated Plant Sperm Cells into Embryo SAC Cells Using a Microforge

    • C. J. Keijzer
    Pages 11-17

  4. Application of the Firefly Luciferase Reporter Gene to Microinjection Experiments in Xenopus Oocytes

    • Michael R. H. White, Martin Braddock, Elaine D. Byles, Lorene Amet, Alan J. Kingsman, Susan M. Kingsman
    Pages 19-28

  5. Application of Confocal Microscopy for the Study of Neuronal Organization in Human Cortical Areas after Microinjection of Lucifer Yellow

    • Pavel V. Belichenko, Annica Dahlstrom, Patrick Sourander
    Pages 29-35

  6. The Dye-Transfer Assay Permits the Evaluation of the Modulation of Junctional Communication by Chemicals: Results Obtained Employing a Highly Sensitive Video-Recording System Connected with the Microinjector

    • Giovanna Mazzoleni, Anna Camplani, Paola Telò, Silvia Tanganelli, Giovanni Ragnotti
    Pages 37-43

  7. Comparison of Fluorescent Dye Transfer in Intercellular Communication Studies Using Scrape-Loading and Microinjection Techniques

    • E. Honikman-Leban, M. M. Shahin
    Pages 45-52

  8. Characterization of the Zea Mays Embryo Sac Using Fluorescent Probes and Microinjection of Lucifer Yellow into the Female Cells

    • E. Matthys-Rochon, C. Digonnet, C. Dumas
    Pages 53-60

  9. Fluorescent Probes — Where Do they Go in Cells, and Why?

    • Richard W. Horobin, Fiza Rashid-Doubell
    Pages 61-66

  10. Fluorescent Phospholipids in Membrane and Lipoprotein Research

    • A. Hermetter, R. Gorges, J. Loidl, F. Paltauf
    Pages 67-72

  11. Selection of Fluorescent Golgi Complex Probes Using Structure-Activity Relationship Models

    • Fiza Rashid-Doubell, Richard W. Horobin
    Pages 73-78

  12. Fluorescent Probes for the Assessment of the Subcellular Effects of Chemicals and Disease Processes In Vivo and In Vitro

    • Peter H. Bach, C. Hugh Reynolds, Stephen Brant
    Pages 79-97

  13. Whole-Cell Patch-Clamping: Introducing Substances into Cells During Electrical Measurements from the Cell Membrane -A Review of Potential Difficulties in Plant and Animal Cells

    • Bert Van Duijn, Can Ince, Zheng Wang, A. Freek Weidema, Kees R. Libbenga, Dirk L. Ypey
    Pages 99-109

  14. Fluorescent Analysis of Replication and Intermediates of Chromatin Folding in Nuclei of Mammalian Cells

    • Gaspar Banfalvi
    Pages 111-119

  15. Autofluorescence in Potato Tuber Phellem: Location and Time Course of Appearance During Tuber Formation

    • Theo Hendriks, Jan-Wouter van Eck, Harrie A. Verhoeven
    Pages 121-127

  16. Flow Cytometric Assessment of Gentamicin Nephrotoxicity in Established Renal Cell Lines

    • H. Mc Glynn, E. Healy, T. Bedell, M. P. Ryan
    Pages 129-132

  17. The Use of the Fluorescent Probe Fura-2 For Intracellular Free Calcium Measurements: Some Methodological Aspects

    • Anneke Wiltink, Arnoud van der Laarse, Nel P. M. Herrmann-Erlee, Joke M. van der Meer, Dirk L. Ypey
    Pages 133-142

  18. Studies on the Effects of Peroxisome Proliferators as Novel Ca2+ Mobilizing Agents Using Indo-1-Loaded Hepatocytes

    • Anton M. Bennett, Oliver P. Flint, Gary M. Williams
    Pages 143-151

  19. Confocal Fluorescence Ratio Imaging of pH in Plant Cells

    • M. D. Fricker, M. R. Blatt, N. S. White
    Pages 153-163

  20. Analyses of the Calcium and Phosphorus Distribution in Predentine Tissue of Rat Incisors

    • H. P. Wiesmann, U. Plate, H. J. Höhling, R. H. Barckhaus
    Pages 165-171
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Keywords

About this book

Individual cells behave in surpnsmg ways that cannot be deduced from the averaged results of an organ as assessed by the use of conventional biochemical methods. Thus multicellular plant and animals systems are being investigated by an increasing array of histochemical and cytochemical techniques based on general chemical or specific immunological interactions to identify structural materials and to assess biological activities. In recent years there has been an increasing range of fluorescent probes, along with advanced computerised imaging and analysis techniques, which allows the behaviour of individual living cells to be followed in considerable detail. The parallel use of microinjection, microelectrodes and patch-clamping provides additional information about cells and their responses. Recombinant DNA technology has highlighted the desirability and the power of microinjecting defined materials into specific cells and so manipulating their fundamental biochemistry. New hypotheses are being tested which will form the cornerstone of future developments across the whole spectrum of biotechnology. The First European Workshop on Biotechnology Applications of Microinjection, Microscopic Imaging and Fluorescence was run at the University of East London, U.K, 21st-24th April, 1992 with the objective of bringing together a diverse group of individuals who were using these state-of-the-art applications for biotechnological exploration. A novel feature of the meeting was paiticipation by instrument manufacturers in the programme: there were hands-on workshops (where living cells could be examined), combined with the poster sessions.

Editors and Affiliations

  • University of East London, London, UK

    Peter H. Bach, C. Hugh Reynolds, Jessica M. Clark, John Mottley, Phil L. Poole

Bibliographic Information

  • Book Title: Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence

  • Editors: Peter H. Bach, C. Hugh Reynolds, Jessica M. Clark, John Mottley, Phil L. Poole

  • DOI: https://doi.org/10.1007/978-1-4615-2828-9

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 1993

  • Hardcover ISBN: 978-0-306-44497-5Published: 30 September 1993

  • Softcover ISBN: 978-1-4613-6223-4Published: 28 October 2012

  • eBook ISBN: 978-1-4615-2828-9Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XI, 255

  • Topics: Pharmacology/Toxicology, Biochemistry, general, Biotechnology, Animal Anatomy / Morphology / Histology, Plant Sciences

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