Overview
- Step-by-step instructions in a laboratory bench protocol format
- Detailed lists of the necessary equipment and reagents
- Tips on troubleshooting from actual experience of the experts
- Example data set in most chapters
- Variety of organisms and applications
Part of the book series: Methods in Molecular Biology (MIMB, volume 442)
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Table of contents (18 protocols)
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Application of RNAi in Diverse Organisms
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Application of RNAi in Diverse Organisms
Keywords
About this book
RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants.
Cutting edge and clearly written, RNAi: Design and Applications enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field.
Editors and Affiliations
Bibliographic Information
Book Title: RNAi
Book Subtitle: Design and Application
Editors: Sailen Barik
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-191-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-874-4Published: 03 April 2008
Softcover ISBN: 978-1-61737-819-5Published: 19 January 2011
eBook ISBN: 978-1-59745-191-8Published: 26 March 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 270
Number of Illustrations: 45 b/w illustrations, 3 illustrations in colour
Topics: Biochemistry, general, Human Genetics, Molecular Medicine, Cell Biology