Overview
- Covers a wide range of methods for investigating how mitochondrial genetics affect cellular physiology and human health
- Details unique experimental approaches for studying mtDNA damage and mutation
- An experimental resource for researchers and physicians concerned with mtDNA disorders
- Provides an in depth description of tools and approaches used by leading researchers
- A comprehensive compendium of tools and approaches used in the field
Part of the book series: Methods in Molecular Biology (MIMB, volume 554)
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Table of contents (23 protocols)
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Mitochondrial DNA Replication, Transcription, Translation, and Respiratory Complex Assembly
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Mitochondrial DNA Damage and Repair
Keywords
About this book
Since the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown. Likewise, our understanding of the basic biology of somatic mtDNA mutations continues to improve. In Mitochondrial DNA: Methods and Protocols, Second Edition, specialists from eight countries share their expertise, providing detailed protocols for studying many aspects of mtDNA. The volume is divided into three sections, which cover the transduction of information from mtDNA to functionally active respiratory complexes, mitochondrial reactive oxygen species (ROS) production as well as mtDNA damage and its repair, and the identification and quantification of heteroplasmic mtDNA mutations. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Mitrochondrial DNA: Methods and Protocols, Second Edition promises to aid researchers in further expanding our knowledge of this vital area of cell biology.
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Editors and Affiliations
Bibliographic Information
Book Title: Mitochondrial DNA
Book Subtitle: Methods and Protocols
Editors: Jeffrey A. Stuart
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-521-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-934115-60-2Published: 21 July 2009
Softcover ISBN: 978-1-61779-495-7Published: 06 December 2011
eBook ISBN: 978-1-59745-521-3Published: 10 June 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 404
Number of Illustrations: 73 b/w illustrations, 1 illustrations in colour
Topics: Cell Biology, Human Genetics