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PRINS and In Situ PCR Protocols

  • Book
  • Sep 1996

Overview

Editors:
  1. John R. Gosden

    1. Western General Hospital, Edinburgh, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 71)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Oligonucleotide PRINS DNA Synthesis

    • John R. Gosden, Diane Lawson
    Pages 1-6

  3. Chromosome-Specific PRINS

    • Jean-Paul Charlieu, Franck Pellestor
    Pages 7-12

  4. Bright-Field Microscopic Detection of Oligonucleotide PRINS-Labeled DNA in Chromosome Preparations

    • Ernst J. M. Speel, Diane Lawson, Frans C. S. Ramaekers, John R. Gosden, Anton H. N. Hopman
    Pages 13-22

  5. Analysis of Sperm Aneuploidy by PRINS

    • Franck Pellestor, Jean-Paul Charlieu
    Pages 23-29

  6. PRINS DNA Synthesis on Frozen Tissue Sections

    • Ernst J. M. Speel, Diane Lawson, Frans C. S. Ramaekers, John R. Gosden, Anton H. N. Hopman
    Pages 31-37

  7. Multiple Sequential Oligonucleotide Primed In Situ DNA Syntheses (MULTI-PRINS)

    • John R. Gosden, Diane Lawson
    Pages 39-44

  8. Oligonucleotide PRINS DNA Synthesis on Extended Chromatin Preparations

    • Yoshiro Shibasaki, John R. Gosden
    Pages 45-51

  9. Combined lmmunocytochemistry and PRINS DNA Synthesis for Simultaneous Detection of Phenotypic and Genomic Parameters in Cells

    • Ernst J. M. Speel, Diane Lawson, Frans C. S. Ramaekers, John R. Gosden, Anton H. N. Hopman
    Pages 53-59

  10. Chromosomal PRINS DNA Labeling Combined with Indirect Immunocytochemistry

    • Arthur R. Mitchell
    Pages 61-70

  11. Direct In Situ Single-Copy (DISC)-PCR

    • Deryl L. Troyer, Hongyan Xie, Jie Hu
    Pages 71-76

  12. In Situ PCR to Cells and to Wax Sections

    • Christiane Mougin, Jean Marc Didier, Dominique Bettinger, Laurence Madoz, Sylviane Coumes-Marquet, Michel Lab
    Pages 77-97

  13. Reverse Transcriptase PCR In Situ on Cryopreserved Tissue Sections

    • Fred J. Hendler, Alice Shum-Siu, Briana W. Heniford, Mohammed El Mekki Ahmed, V. J. Patel
    Pages 99-111

  14. Flow Cytometry Analysis of an In Situ PCR for the Detection of Human Immunodeficiency Virus Type-1 (HIV-1) Proviral DNA

    • David E. Gibellini, Maria Carla Re, Giuliano Furlini, Michele La Placa
    Pages 113-122

  15. In Situ PCR Amplification of Intracellular mRNA

    • Raymond H. Chen, Susan V. Fuggle
    Pages 123-132

  16. Localized In Situ Amplification (LISA)

    • Gregory J. Tsongalis
    Pages 133-139

  17. In Situ PCR

    • Aldan A. Long, Paul Komminoth
    Pages 141-161

  18. Back Matter

    Pages 163-165
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Keywords

About this book

Cutting edge researchers demonstrate step-by-step how oligonucleotide primers may be successfully used to detect and amplify or extend complimentary sequences in situ. Through these procedures-often invented by the authors-the door is opened to rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs in cells-at hitherto unmatched degrees of sensitivity and specificity. Their innovative techniques-suitable for both novice and experienced researchers-have rapidly become indispensable for many clinical diagnostic procedures, whether in quantification of chromosomes in the identification of aneuploidy for prenatal diagnosis, or in the identification of viral infection in the early stages, or of rare messenger RNAs present in cells.

Reviews

...[a] splendid little book which covers the field quite well...Chapetr 16, which is a general review of in situ PCR written by Aidan Long and Paul Komminoth...is by far the best review of the approach...yet seen and should be mandatory reading for anyone intending to try in situ PCR. -FEBS Letters

"...useful to all those increasingly applying such techniques to in situ analysis...this book contains clear practical instructions that are easy to follow, and is well illustrated. It is essential for any laboratory contemplating exploiting this powerful technique."-SGM Quarterly

Editors and Affiliations

  • Western General Hospital, Edinburgh, UK

    John R. Gosden

Bibliographic Information

  • Book Title: PRINS and In Situ PCR Protocols

  • Editors: John R. Gosden

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033953

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1997

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 165

  • Number of Illustrations: 53 b/w illustrations, 22 illustrations in colour

  • Topics: Cell Biology

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