Overview
- Editors:
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John R. Stephenson
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London School of Hygiene and Tropical Medicine, University of London, UK
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Alan Warnes
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Micron Bioproducts, Camberley, UK
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Table of contents (26 protocols)
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Front Matter
Pages i-xiii
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- Tanvir Tabish, Alan Warnes, Stuart Clark
Pages 1-6
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- John T. Roehrig, Teresa M. Brown, Alison J. Johnson, Nick Karabatsos, Denise A. Martin, Carl J. Mitchell et al.
Pages 7-18
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- Xi Jiang, David O. Matson
Pages 19-27
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- Dennis W. Trent, Gwong-Jen Chang, A. Vance Vorndam, Robert S. Lanciotti
Pages 51-61
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- Mark J. Espy, P. Shawn Mitchell, David H. Persing, Thomas F. Smith
Pages 89-101
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- Sharon Cassol, Stanley Read, Bruce G. Weniger, Richard Pilon, Barbara Leung, Theresa Mo
Pages 103-117
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- Joanna S. Ellis, David W. G. Brown
Pages 119-127
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- Ilkka Julkunen, Irja Davidkin, Christian Oker-Blom
Pages 143-157
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- Sandra H. Kirk, David T. Y. Liu
Pages 159-171
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- Karen E. Hicks, Stuart Beard, Bernard J. Cohen, Jonathan P. Clewley
Pages 173-188
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- Hermann Meyer, Susan L. Ropp, Joseph J. Esposito
Pages 199-211
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- M. Sofi Ibrahim, Connie S. Schmaljohn
Pages 231-243
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- Pekka Halonen, Timo Lövgren
Pages 245-255
About this book
The accurate and reliable diagnosis of transmissible diseases is the most powerful weapon available to ensure their control, and in some cases eradication. The detection of parasites in clinical cases, companion and farm animals, and in the environment is relatively easy since many of them are visible to the naked eye, and those that are not are readily detected by light microscopy. Fungal infections can similarly be determined. Bacteria are somewhat harder to detect. Although their presence can frequently be detected by light microscopy, differential diagnosis, beyond their gross morphology, is almost always impossible. However, most bacterial pathogens can be cultured in the laboratory and can be accurately identified by combinations of a series of simple tests such as morphology, staining, antibiotic sensitivity, biochemical analyses, nutrient dependence, and phage sensitivity. Viruses, however, have proved much more difficult; their size and absolute dependence of the host cell for propagation have rendered useless the methods traditionally used for other microorganisms. Until the development of tissue culture in the middle of this century, diagnosis was entirely dependent on the skill and experience of the clinician. But this was an unreliable process since many of the common virus infections exhibit similar clinical symptoms, such as coryza, exanthema, vomiting, diarrhea, neuralgia, and lethargy. Indeed many viral infections display clinical signs that are indistinguishable from bacterial or parasitic infections.
Reviews
". . .an invaluable source of information for clinical and non-clinical virologists and microbiologists. . .would be a good asset to most research laboratories. . .For those that do perform these assays, it is invaluable."-Doody's Health Sciences Book Review Journal
Editors and Affiliations
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London School of Hygiene and Tropical Medicine, University of London, UK
John R. Stephenson
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Micron Bioproducts, Camberley, UK
Alan Warnes