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Hormone Assays in Biological Fluids

  • Book
  • © 2006

Overview

Editors:
  1. Michael J. Wheeler

    1. St. Thomas’ Hospital, Guy’s and St. Thomas’ NHS Foundation Trust, London, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. J. S. Morley Hutchinson

    1. University of Aberdeen, Aberdeen, UK

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 324)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-ix
    Download chapter PDF

  2. Immunoassay Techniques

    • Michael J. Wheeler
    Pages 1-23

  3. High-Performance Liquid Chromatography for Hormone Assay

    • John W. Honour
    Pages 25-52

  4. Gas Chromatography-Mass Spectrometry

    • John W. Honour
    Pages 53-74

  5. An Introduction to PCR Techniques

    • Gill Rumsby
    Pages 75-89

  6. Methods for the Investigation of Thyroid Function

    • Christine R. Squire
    Pages 91-108

  7. Assays for LH, FSH, and Prolactin

    • Michael J. Wheeler
    Pages 109-124

  8. Adrenocorticotrophic Hormone and Related Peptides

    • Les A. Perry
    Pages 125-139

  9. Measurement of Glucocorticoids in Biological Fluids

    • Geoff Holder
    Pages 141-157

  10. Urinary Steroid Profiling

    • Norman F. Taylor
    Pages 159-175

  11. Measurement of Aldosterone in Blood

    • Marion L. Cawood
    Pages 177-185

  12. Measurement of Plasma Renin Activity

    • Marion L. Cawood
    Pages 187-196

  13. Measurement of Androgens

    • Michael J. Wheeler
    Pages 197-211

  14. Measurement of Gut Hormones in Plasma

    • Shahrad Taheri, Mohammad Ghatei, Steve Bloom
    Pages 213-233

  15. Measurement of Melatonin and 6-Sulphatoxymelatonin

    • Benita Middleton
    Pages 235-254

  16. Back Matter

    Pages 255-258
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Keywords

About this book

Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can set up novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.

Reviews

From the reviews:

"Interest in hormone diagnostics has developed dramatically over the last decades … . In summary, this book is written by knowledgeable experts in the field of hormone analysis in biological fluids … . It provides a valuable collection of assays, described in a practical way, so they can be rapidly implemented by laboratorians looking for hormone assays suitable for research purposes or as alternatives to commercial immunoassays." (Linda M. Thienpont, Clinical Chemistry, Vol. 52 (9), 2006)

 

"...a wonderful resource for all thoe who, at some point, face decisions about the most suitable hormone assay for a particular situation." -TRENDS in Endocrinology and Metabolism

Editors and Affiliations

  • St. Thomas’ Hospital, Guy’s and St. Thomas’ NHS Foundation Trust, London, UK

    Michael J. Wheeler

  • University of Aberdeen, Aberdeen, UK

    J. S. Morley Hutchinson

Bibliographic Information

  • Book Title: Hormone Assays in Biological Fluids

  • Editors: Michael J. Wheeler, J. S. Morley Hutchinson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599869

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-005-2Published: 16 January 2006

  • Softcover ISBN: 978-1-61737-299-5Published: 10 November 2010

  • eBook ISBN: 978-1-59259-986-8Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: IX, 258

  • Topics: Biochemistry, general

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