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Two-Dimensional Imaging and Spatiotemporal Analysis of Biophoton

Technique and applications for biomedical imaging

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Biophotonics

4. Conclusion

In this chapter, our studies focused on techniques and instrumentation for highly sensitive and precise determination of biophotons through imaging and their applications, investigated from various viewpoints, are discussed. Applications based on these techniques are introduced over a range of subjects, from the level of microbes to that of humans. Although the detailed mechanisms of biophoton emission in each case have not yet been clarified sufficiently, the experimental results introduced here show a high potential for analyzing biophotons for the quantification of physiological and pathological information. The most valuable merit of analyzing biophotons is that it is apparently a non-invasive technique, and it cannot be influenced in the same way as other methods that use extrinsic physical probes. However, the weakness of the intensity of biophoton, which may restrict its application, requires a breakthrough in analytical technique. In that sense, I believe that our technologies for the physical analysis of biophotons could form the basis for a novel stage in biophoton research. In particular, the statistical characterization of biophotons with spatiotemporal determination will provide a novel approach by which biological order emerged with the interaction of biological reactions can be characterized, even when the photon density is very low.

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6. References

  1. L. Colli and U. Facchini, Light emission by germinating plants, IL Nuovo Cimento 12, 150–153 (1954).

    Article  Google Scholar 

  2. F. A. Pop (Ed.), Special issue on biophoton, Experientia 44, 543–600 (1988).

    Google Scholar 

  3. H. Inaba, New bio-information from ultraweak photon emission in life and biological activities: biophoton, in: Modern Radio Science 1990, edited by J.B. Andersen (Oxford Univ. Press, Oxford, 1990), pp.164–184 and references cited therein.

    Google Scholar 

  4. M. Kobayashi and H. Inaba, Photon statistics and correlation analysis of ultraweak light originating from living organisms for extraction of biological information, Appl. Otp. 39(1), 183–192 (2000).

    Article  CAS  Google Scholar 

  5. M. Kobayashi, M. Usa, and H. Inaba, Highly sensitive detection and spectral analysis of ultraweak photon emission from living samples of human origin for the determination of biomedical information, Trans. of SICE E-1, 214–220 (2001).

    Google Scholar 

  6. M. Kobayashi, B. Devaraj, and H. Inaba, Observation of super-Poisson statistics of bacterial (Photobacterium phosphoreum) bioluminescence during the early stage of cell proliferation, Phys. Rev. E 57(2), 2129–2133 (1998).

    Article  CAS  Google Scholar 

  7. M. Kobayashi, B. Devaraj, M. Usa, Y. Tanno, M. Takeda, and H. Inaba, Development and applications of new technology for two-dimensional space-time characterization and correlation analysis of ultraweak biophoton information, Frontiers Med. Biol. Engng. 7(4), 99–309 (1996).

    Google Scholar 

  8. M. Kobayashi, T. Takeda, K-I. Ito, H. Kato, and H. Inaba, Two-dimensional photon counting imaging and spatiotemporal characterization of ultraweak photon emission from a rat’s brain in vivo, J. Neurosci. Method 93(2), 163–168 (1999).

    Article  CAS  Google Scholar 

  9. M. Kobayashi, M. Takeda, T. Sato, Y. Yamazaki, K. Kaneko, K-I. Ito, H. Kato, and H. Inaba, In vivo imaging of spontaneous ultraweak photon emission from a rat’s brain correlated with cerebral energy metabolism and oxidative stress, Neurosci. Res. 34(2), 103–113 (1999).

    Article  CAS  Google Scholar 

  10. M. Kobayashi, B. Devaraj, M. Usa, Y. Tanno, M. Takeda, and H. Inaba, Two-dimensional imaging of ultraweak photon emission from germinating soybean seedlings with a highly sensitive CCD camera. Photochem. Photobiol. 65(3), 535–537 (1997).

    CAS  Google Scholar 

  11. CD. Mackey, The role of charge coupled devices in low light level imaging, in: A Technical Guide from Astromed Limited, Issue 1, pp. 1–25 (1991)

    Google Scholar 

  12. T. B. Suslova, V. I. Olenev, and Y.A. Vladimirov, Chemiluminescence coupled with the formation of lipid peroxides in biological membranes-I. Luminescence of mitochondria on addition of Fe++, Biofizika 14, 540–548 (1969).

    Google Scholar 

  13. E. Cadenas, A. Boveris, and Chance, B, Low-level chemiluminescence of bovine heart submitochondrial particles, Biochem. J. 186, 659–667 (1980).

    CAS  Google Scholar 

  14. E. Hideg, M. Kobayashi, and H. Inaba, Spontaneous ultraweak light emission from respiring spinach leaf mitochondria, Biochim. Biophys. Acta. 1098, 27–31 (1991).

    Article  CAS  Google Scholar 

  15. T. G.. Mamedov, G.A. Podov, and V.V. Konev, Ultraweak luminescence of various organisms, Biofizika 14, 1047–1051 (1969).

    Google Scholar 

  16. M. Takeda, Y. Tanno, M. Kobayashi, M. Usa, and H. Inaba, A novel method of assessing carcinoma cell proliferation by biophoton emission, Cancer Lett. 127(1–2), 155–160 (1998).

    Article  CAS  Google Scholar 

  17. T. Amano, M. Kobayashi, B. Devaraj, M. Usa, and H. Inaba, Ultraweak Biophoton Emission Imaging of Transplanted Bladder Cancer. Urol. Res. 23, 315–318 (1995).

    Article  CAS  Google Scholar 

  18. E. Cadenas, A. Boveris, and B. Chance, Low-level chemiluminescence of biological systems, in: Free radicals in biology, Vol. VI, edited by W. A. Proyor (Academic Press, New York, 1984), pp. 211–242.

    Google Scholar 

  19. A. Boveris, E. Cadenas, R. Reiter, M. Filipkowski, Y. Nakase, and B. Chance, Organ chemiluminescence: Noninvasive assay for oxidative radical reactions, Proc. Natl. Acad. Sci. USA 77, 347–351 (1980).

    Article  CAS  Google Scholar 

  20. M. L. Salin, K.L. Quince, and D.J. Hunter, Chemiluminescence from mechanically injured soybean root tissue. Photobiochem. Photobiophys. 9, 271–279 (1985).

    CAS  Google Scholar 

  21. S. Suzuki, M. Usa, T. Nagoshi, M. Kobayashi, N. Watanabe, H. Watanabe, and H. Inaba, Two-dimensional imaging and counting of ultraweak emission patterns from injured plant seedlings, J. Photochem. Photobiol. B:Biol. 9, 211–217 (1991).

    Article  Google Scholar 

  22. M. Kobayashi, Highly sensitive detection of reactive oxygen species originating in living body based on biophoton detection, Optronics, No.12, pp. 139–147 (1999) (in Japanese).

    Google Scholar 

  23. M. Takeda, M. Kobayashi, M. Takayama, S. Suzuki, T. Ishida, K. Ohnuki, T. Moriya, and N. Ohuchi, (2004) (submitted).

    Google Scholar 

  24. G. Sauermann, W. Mei, U. Hoppe, and F. Stab, Ultraweak photon emission of human skin in vivo: Influence of topically applied antioxidants on human skin, in: Methods in Enzym. 300, edited by L. Packer (Academic Press, New York, 1999), pp. 419–428.

    Google Scholar 

  25. S. Cohen and F. A. Pop, Biophoton emission of the human body, J. Photochem. Photobiol. B:Biol. 40, 187–189 (1997).

    Article  CAS  Google Scholar 

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Kobayashi, M. (2005). Two-Dimensional Imaging and Spatiotemporal Analysis of Biophoton. In: Shen, X., Van Wijk, R. (eds) Biophotonics. Springer, Boston, MA . https://doi.org/10.1007/0-387-24996-6_12

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