Abstract
As part of a pilot project, roughly-cut slices of ductal carcinoma from human mastectomies were imaged using acoustic microscopy in the range 15 to 50 MHz. C-scan images of the 2 to 5 mm thick specimens were shown to correlate well with corresponding optical images and showed higher contrast. By selective gating, internal structures could be highlighted in C-scan images that were not visible from the surfaces of the slice. Velocity and attenuation image maps of the sample were also made and then used to try to identify the tumor regions based quantitative criteria. Our results suggest that acoustic microscopy shows promise as an adjunctive method for pathologists to rapidly measure tumor size and resection margin status during intraoperative consultation and provide more complete specimen assessment during gross examination
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References
M.E. Anderson, M.S. Soo, R.C. Bentley, and G.E. Trahey, The detection of breast micro-calcifications with medical ultrasound, J. Acoust. Soc. Amer. 101, 29–39 (1997).
M.P. André, M. Galperin, L.K. Olson, K. Richman, S. Payrovi, and P. Phan, Improving the accuracy of diagnostic breast ultrasound in: Acoustical Imaging, v. 26, edited by R. Gr. Maev (Kluwer Academic/Plenum Press, New York, 2002), pp. 453–460,.
R.A. Lemons and C.F. Quate, Acoustic microscopy: Biomedical application, Science 188, 905–914 (1975).
G. Kossoff, E.K. Fry, and J. Jellins, Average velocity of ultrasound in the human female breast, J. Acoust. Soc. Am. 53, 1730–1736 (1973).
F.S. Foster, M. Strban, and G. Austin, The ultrasound macroscope: Initial studies of breast tissue, Ultrasonic Imaging 6, 243–261 (1984).
F.T. D’Astous, and F.S. Foster, Frequency dependence of ultrasound attenuation and backscatter in breast tissue, Ultrasound Med. Biol. 12, 795–808 (1986).
P.D. Edmonds, C.L. Mortensen, J.R. Hill, S.K. Holland, J.F. Jensen, P. Schattner, A.D. Valdes, R.H. Lee, and F.A. Marzoni, Ultrasound tissue characterization of breast biopsy specimens, Ultrasonic Imaging 13, 162–185 (1991).
C.L. Mortensen, P.D. Edmonds, Y. Gorfu, J.R. Hill, J.F. Jensen, P. Schattner, L.A. Shifrin, A.D. Valdes, S.S. Jeffrey, and L.J. Esserman, Ultrasound tissue characterization of breast biopsy specimens: Expanded study, Ultrasonic Imaging 18, 215–230 (1996).
C. Calderon, D. Vilkomerson, R. Mezrich, K.F. Etzold, and M. Haskin, Differences in the attenuation of ultrasound by normal benign and malignant breast tissue, J. Clin. Ultrasound 4, 249–254 (1976).
E. Kelly Fry, N.T. Sanghvi, and F.J. Fry, Frequency dependent attenuation of malignant breast tumors studied by the fast Fourier transform technique, in: Ultrasonic Tissue Characterization II, NBS Spec. Publ. 525, edited by M. Linzer (U.S. Government Printing Office, Washington, DC, 1979), pp. 85–91.
R.M. Golub, R.E. Parsons, B. Sigel, E.J. Feleppa, J. Justin, H.A. Zaren, M. Rorke, J. Sokil-Melgar, and H. Kimitsuki, Differentiation of breast tumors by ultrasonic tissue characterization, J. Ultrasound Med. 12, 601–608 (1993).
T.M. Burke, T.A. Blankenberg, A.K.Q. Siu, F.G. Blankenberg, and H.M. Jensen, Preliminary investigation of ultrasound scattering analysis to identify women at risk for later invasive cancer, Ultrasound Med. Biol. 21, 295–303 and 305–311 (1995).
M.E. Anderson, M.S.C. Soo, and G.E. Trahey, In vivo breast tissue backscatter measurements with 7.5- and 10-MHz transducers, Ultrasound Med. Biol. 27, 75–81 (2001).
L.A. Denisova, R.Gr. Maev, I.V. Matveichuk, Yu.I. Denisov-Nikolsky, A.A. Denisov, and E.Yu. Maeva, Investigating bone microstructure and mechanical properties using acoustic microscopy in: Acoustic Imaging, v. 26, edited by R. Gr. Maev (Kluwer Academic/Plenum Press, New York, 2002), pp. 61–68.
R. Maev, L. Denisova, E. Maeva, and A. Denissov, New data on histology and physico mechanical properties of human tooth tissue obtained with acoustic microscopy, Ultrasound Med. Biol. 28, 131–136 (2002).
E. Maeva, I. Bruno, M. Docker, B. Zielinsky, F. Severin, and R.Gr. Maev, Method of acoustic microscopy for sex determination of sea lamprey, petromyzon marinus larvae, J. Fish Biol. 65, 148–156 (2004).
R.E. Kumon, I. Bruno, B. Heartwell, and E. Maeva, Breast tissue characterization with high-frequency scanning acoustic microscopy, J. Acoust. Soc. Am. 115, 2376(A) (2004).
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Bruno, I., Kumon, R., Heartwell, B., Maeva, E., Maev, R. (2007). Ex Vivo Breast Tissue Imaging and Characterization Using Acoustic Microscopy. In: André, M.P., et al. Acoustical Imaging. Acoustical Imaging, vol 28. Springer, Dordrecht. https://doi.org/10.1007/1-4020-5721-0_29
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DOI: https://doi.org/10.1007/1-4020-5721-0_29
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