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Planar Protein Arrays in Microtiter Plates: Development of a New Format Towards Accurate, Automation-Friendly and Affordable (A3) Diagnostics

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Molecular Diagnostics

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 133))

Abstract

A technology for protein microarrays in 96 well microplates has been developed as a widely adoptable platform for multiplexed protein based diagnostics. Procedures for protein microarray manufacturing, immobilization, assay optimization and optical detection methods were developed. Using a clever combination of state of the art technologies, a versatile platform that works with peptides, proteins and antibodies as capture agents in a scalable format for planar arrays is described.

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Author information

Authors and Affiliations

  1. Scienion AG, Volmerstr. 7b, 12489, Berlin, Germany

    Holger Eickhoff

  2. Microdiscovery GmbH, Marienburger Strasse 1, 10405, Berlin, Germany

    Arif Malik

Authors
  1. Holger Eickhoff
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  2. Arif Malik
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Corresponding author

Correspondence to Holger Eickhoff .

Editor information

Editors and Affiliations

  1. Fraunhofer-Institut für Biomed. Technik, Am Mühlenberg 13, Potsdam, 14476, Germany

    Harald Seitz

  2. Fraunhofer Institut für Biomedizinische, Am Mühlenberg 13, Potsdam, 14476, Germany

    Sarah Schumacher

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© 2012 Springer-Verlag Berlin Heidelberg

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Eickhoff, H., Malik, A. (2012). Planar Protein Arrays in Microtiter Plates: Development of a New Format Towards Accurate, Automation-Friendly and Affordable (A3) Diagnostics. In: Seitz, H., Schumacher, S. (eds) Molecular Diagnostics. Advances in Biochemical Engineering/Biotechnology, vol 133. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2012_148

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