Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Abbreviations
- \( a \) :
-
Empirical value for mathematical k L a description
- \( A \) :
-
Heat transfer area
- AOX:
-
Alcohol oxidase
- \( b \) :
-
Empirical value for mathematical k L a description
- \( \Updelta c \) :
-
Concentration difference
- \( \Updelta c_{0} \) :
-
Initial concentration difference
- \( C(t) \) :
-
Dissolved oxygen concentration
- \( C^{*} \) :
-
Oxygen saturation concentration
- CQA:
-
Critical quality attributes
- \( d_{1} \) :
-
Vessel diameter
- \( d_{2} \) :
-
Impeller diameter
- DCU:
-
Digital control unit
- DCW:
-
Dry cell weight
- E. coli :
-
Escherichia coli
- \( F(t) \) :
-
Feed flow rate
- F G :
-
Gas flow rate
- HCDF:
-
High cell density fermentation
- IPTG:
-
Isopropyl-β-D-thiogalactopyranoside
- \( k \) :
-
Heat transfer coefficient
- \( k_{L} a \) :
-
Volumetric mass transfer coefficient
- KPP:
-
Key process parameters
- \( M \) :
-
Goodness of mixture
- \( n \) :
-
Stirrer speed
- \( Ne \) :
-
Newton number
- OD600 :
-
Optical density measured at 600 nm
- \( OTR \) :
-
Oxygen transfer rate
- \( OUR \) :
-
Oxygen uptake rate
- \( P/V \) :
-
Power input per volume
- P. pastoris :
-
Pichia pastoris
- pO2 :
-
Oxygen partial pressure
- \( q_{{O_{2} }} \) :
-
Specific oxygen uptake rate
- \( Q \) :
-
Heat flow
- \( Q_{prod} \) :
-
Released heat flow
- QbD:
-
Quality by design
- RM:
-
Rocking motion
- RO:
-
Reverse osmoses
- \( s \) :
-
Thickness of the reactor wall
- S :
-
Correlation factor for the heat generation
- \( S_{Feed} \) :
-
Concentration of the feed solution
- SDS-PAGE:
-
Sodium dodecyl sulfate polyacrylamide gel electrophoresis
- STR:
-
Stirred tank reactor
- t :
-
Time
- \( \Updelta T \) :
-
Temperature difference
- u :
-
Tip speed
- \( V \) :
-
Filling volume of the bioreactor
- \( V_{0} \) :
-
Initial volume
- WCW:
-
Wet cell weight
- \( X \) :
-
Cell density (dry cell weight)
- \( X_{0} \) :
-
Initial cell density
- \( Y_{{X/O_{2} }} \) :
-
Oxygen yield coefficient
- \( Y_{X/S} \) :
-
Yield coefficient
- \( Z \) :
-
Empirical value for mathematical k L a description
- \( \alpha_{1} \) :
-
Convective heat transfer of the cooling liquid inside the double wall
- \( \alpha_{2} \) :
-
Convective heat transfer of the reactor wall into the medium
- \( \lambda \) :
-
Wall thermal conductivity
- \( \mu \) :
-
Specific growth rate
- \( \mu_{set} \) :
-
Selected specific growth rate for the fed batch
- ρ :
-
Density
- θ:
-
Mixing time
- \( \nu \) :
-
Superficial air velocity
References
Waegeman H, Mey HD (2012) Increasing recombinant protein production in E. coli by an alternative method to reduce acetate. Advances in Applied Biotechnology. http://www.intechopen.com/books/advances-in-appliedbiotechnology/increasing-recombinant-protein-production-in-e-coli-by-an-alternative-method-to-reduce-acetate. Accessed 27 Jan 2013
Walsh G (2010) Biopharmaceutical benchmarks 2010. Nat Biotechnol. doi:10.1038/nbt0803-865
Lee S (1996) High cell-density culture of Escherichia coli. Elsevier Science Ltd. doi:10.1016/0167-7799(96)80930-9
Cregg J.M. (2007) Methods in molecular biology.In: Pichia protocols, vol 389 2nd edn, pp 119–138. Humana Press, Totowa
Chen R (2011) Bacterial expression systems for recombinant proteins E. coli and beyond. Biotechnol Adv. doi:10.1016/j.biotechadv.2011.09.013
Mücke M, Ostendorp R, Leonhartsberger S (2009) E. coli secretion technologies enable production of high yields of active human antibody fragments. BioProcess Int 9:2–6
Eibl R, Werner S, Eibl D (2009) Bag bioreactor based on wave-induced motion: characteristics and applications. Adv Biochem Engin/Biotechnol. doi:10.1007/10_2008_15
Brecht R (2009) Disposable bioreactors maturation into pharmaceutical manufacturing. Adv Biochem Engin/Biotechnol. doi:10.1007/10_2008_33
Eibl D, Peuker T, Eibl R (2010) Single-use equipment in biopharmaceutical manufacture: a brief introduction. In: Eibl R, Eibl D (eds) Single-use technology in biopharmaceutical manufacture. Wiley, Hoboken
Eibl R, Eibl D (2009) Application of disposable bag bioreactors in tissue engineering and for the production of therapeutic agents. Adv Biochem Engin/Biotechnol 112:183–207. doi:10.1007/10_2008_3
Glazyrina J, Materne E, Dreher T, Storm D, Junne S, Adams T, Greller G, Neubauer P (2010) High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor. Microb Cell Fact. doi:10.1186/1475-2859-9-42
Singh V (1998) Disposable bioreactor for cell culture using wave-induced agitation. Cytotechnology. doi:10.1023/A:1008025016272
Eibl R, Kaiser S, Lombriser R, Eibl D (2010) Disposable bioreactors: the current state-of-the-art and recommended applications in biotechnology. Appl Microbiol Biotechnol. doi:10.1007/s00253-009-2422-9
Mikola M, Seto J, Amanullah A (2007) Evaluation of a novel wave bioreactor cellbag for aerobic yeast cultivation. Bioprocess Biosyst Eng. doi:10.1007/s00449-007-0119-y
Dreher T, Husemann U, Zahnow C, Wilde DD, Adams T, Greller G (2013) High cell density Escherichia coli cultivation in different single-use bioreactor systems. Chem Ing Tech. doi:10.1002/cite.201200122
Terrier B, Courtois D, Hénault N, Cuvier A, Bastin M, Aknin A, Dubreuil J, Pétiard V (2007) Two new disposable bioreactors for plant cell culture: the wave and undertow bioreactor and the slug bubble bioreactor. Biotechnol Bioeng doi:10.1002/bit.21187
Junne S, Solymosi T, Oosterhuis N, Neubauer P (2013) Cultivation of cells and microorganisms in wave-mixed disposable bag bioreactors at different scales. Chem Ing Tech. doi:10.1002/cite.201200149
Galliher PM, Hodge G, Guertin P, Chew C, Deloggie T (2010) Single-use bioreactor platform for microbial fermentation in single-use technology in biopharmaceutical manufacture. In: Eibl R, Eibl D (eds) Single-use technology in biopharmaceutical manufacture. Wiley, Hoboken
Noack U, Verhoeye F, Kahlert W, Wilde DD, Greller G (2010) Disposable stirred tank reactor BIOSTAT® CultiBag STR. In: Eibl R, Eibl D (eds) Single-use technology in biopharmaceutical manufacture. Wiley, Hoboken
Krause M, Ukkonen K, Haataja T, Ruottinen M, Glumoff T, Neubauer A, Neubauer P, Vasala A (2010) A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures. Microb Cell Fact. doi:10.1186/1475-2859-9-11
Juran JM (1992) Juran on quality by design. Free Press, New York
CMC Biotech Working Group (2009) A-mab: a case study in bioprocess development. www.ispe.org/pqli/a-mab-case-study-version-2.1. Accessed 12 Dec 2012
ISPE http://www.ispe.org/glossary?term=Key+Process+Parameter+(KPP) Accessed 12 Dec 2012
Lara AR, Galindo E, Ramirez OT, Palomares A (2006) Living with heterogeneities in bioreactors. Mol Biotech. doi:10.1385/MB:34:3:355
Kraume M. (2002) Mischen und Rühren, Grundlage und moderne Verfahren. Wiley-VCH Verlag, Weinheim
Xing Z, Kenty B, Li Z, Lee S (2009) Scale-up analysis for a CHO cell culture process in large-scale bioreactors. Biotechnol Bioeng. doi:10.1002/bit.22287
Stanbury PF, Whitaker A, Hall J (1995) Principles of fermentation technology. Pergamon Oxford, Oxford
Ochoa FG, Gomez E (2009) Bioreactor scale-up and oxygen transfer rate in microbial processes: an overview. Biotechnol Adv. doi:10.1016/j.biotechadv.2008.10.006
Harrison D, Loveless J (1971) The effect of growth conditions on respiratory activity and growth efficiency in facultative anaerobe grown chemostat culture. J Gen Microbiol. doi:10.1099/00221287-68-1-35
Riesenberg D, Schulz V, Knorre WA, Pohl HD, Korz D, Sanders EA, Roß A, Deckwer WD (1991) High cell density cultivation of Escherichia coli at controlled specific growth rate. J Biotechnol. doi:10.1016/0168-1656(91)90032-Q
Riet KV (1983) Mass transfer in fermentation. Trends Biotechnol. doi:10.1016/0167-7799(83)90034-3
Junker BH (2004) Scale-up methodologies for Escherichia coli and yeast fermentation processes. J Biosci Bioeng. doi:10.1016/S1389-1723(04)70218-2
Shuler ML, Kargi F (2006) Bioprocess engineering: basic concepts, 2nd edn. Prentice Hall PTR, Upper Saddle River
Cooney CL, Wang DIC, Mateles RI (1968) Measurement of heat evolution and correlation with oxygen consumption during microbial growth. Biotechnol Bioeng. doi:10.1002/bit.260110302
VDI-Wärmeatlas (2006) 19th edn, chapter 3, doi: 10.1007/978-3-540-32218-4
Wise WS (1951) The Measurement of the aeration of culture media. J Gen Microbiol. doi:10.1099/00221287
Kocourek A (2002) Darstellung und Charakterisierung der katalytischen Domäne der humanen Makrophagenelastase. http://bieson.ub.uni-bielefeld.de/volltexte/2003/223/pdf/0097.pdf. Accessed 12 Dec 2012
Cereghino JL, Cregg JM (2006) Hetrologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. doi:10.1111/j.15746976.2000.tb00532.x
Eibl D, Eisenkrätzer D (2012) Personal communication
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Dreher, T. et al. (2013). Microbial High Cell Density Fermentations in a Stirred Single-Use Bioreactor. In: Eibl, D., Eibl, R. (eds) Disposable Bioreactors II. Advances in Biochemical Engineering/Biotechnology, vol 138. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2013_189
Download citation
DOI: https://doi.org/10.1007/10_2013_189
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-45157-7
Online ISBN: 978-3-642-45158-4
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)