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Tailoring Fluorescent Labels for Far-Field Nanoscopy

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Far-Field Optical Nanoscopy

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 14))

Abstract

The choice of the fluorophore to be used in super-resolution far-field nanoscopy is an essential parameter that typically determines the quality of the resulting image. To overcome the diffraction limit, different super-resolution techniques exploit different optical and photochemical phenomena, imposing method-specific requirements for useful probes. The choice of the fluorophore is even more intimately tied to performance for super-resolution imaging in live cells, where both photophysical properties and target-specific labeling inside the cell are essential. In this chapter, we highlight the advances in fluorophore development for a range of existing super-resolution techniques. We discuss the requirements these distinct methods place on the fluorophores in order to achieve optimal resolution, particularly as these methods move toward imaging living cells beyond the diffraction limit.

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Authors and Affiliations

  1. 292 Mellon Institute, Molecular Biosensors and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA

    Dmytro A. Yushchenko & Marcel P. Bruchez

Authors
  1. Dmytro A. Yushchenko
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  2. Marcel P. Bruchez
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Correspondence to Marcel P. Bruchez .

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Editors and Affiliations

  1. NanoBioSciences Institute of Physical and Theoretical Chemistry, TU Braunschweig, Braunschweig, Germany

    Philip Tinnefeld

  2. MRC Human Immunology Unit and Wolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom

    Christian Eggeling

  3. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany

    Stefan W. Hell

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Yushchenko, D.A., Bruchez, M.P. (2012). Tailoring Fluorescent Labels for Far-Field Nanoscopy. In: Tinnefeld, P., Eggeling, C., Hell, S. (eds) Far-Field Optical Nanoscopy. Springer Series on Fluorescence, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2011_35

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