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Single-Cell Expression Profiling and Proteomics of Primordial Germ Cells, Spermatogonial Stem Cells, Adult Germ Stem Cells, and Oocytes

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Stem Cells: Biology and Engineering

Part of the book series: Advances in Experimental Medicine and Biology ((ICRRM,volume 1083))

Abstract

The mammalian germ cells, cell assemblies, tissues, and organs during development and maturation have been extensively studied at the tissue level. However, to investigate and understand the fundamental insights at the molecular basis of germ and stem cells, their cell fate plasticity, and determination, it is of most importance to analyze at the large scale on the single-cell level through different biological windows. Here, modern molecular techniques optimized for single-cell analysis, including single fluorescence-activated cell sorting (FACS) and single-cell RNA sequencing (scRNA-seq) or microfluidic high-throughput quantitative real-time polymerase chain reaction (qRT-PCR) for single-cell gene expression and liquid chromatography coupled to tandem mass spectrometry (LC-MSMS) for protein profiling, have been established and are still getting optimized.

This review aims on describing and discussing recent single-cell expression profiling and proteomics of different types of human germ cells, including primordial germ cells (PGCs), spermatogonial stem cells (SSCs), human adult germ stem cells (haGSCs), and oocytes.

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Abbreviations

FACS:

Fluorescence-activated cell sorting

haGSC:

Human adult germ stem cell

hESC:

Human embryonic stem cells

hFib:

Human fibroblast

hPSC:

Human pluripotent stem cell

hSSC:

Human spermatogonial stem cell

LC-MSMS:

Liquid chromatography mass spectrometry

MACS:

Magnetic activated cell sorting

MSCs:

Mesenchymal stem cells

hPGC:

Human primordial germ cell

qRT-PCR:

Quantitative real-time polymerase chain reaction

scRNA-seq:

Single-cell RNA sequencing

hSSC:

Human spermatogonial stem cell

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Author information

Authors and Affiliations

  1. P.O. Box 1243, 72072, Tübingen, Germany

    Sabine Conrad

  2. Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran

    Hossein Azizi

  3. Institute for Anatomy and Cell Biology III, Medical Faculty, Heidelberg University, Heidelberg, Germany

    Thomas Skutella

Authors
  1. Sabine Conrad
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  2. Hossein Azizi
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  3. Thomas Skutella
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Corresponding author

Correspondence to Thomas Skutella .

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Editors and Affiliations

  1. Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam

    Phuc Van Pham

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Conrad, S., Azizi, H., Skutella, T. (2017). Single-Cell Expression Profiling and Proteomics of Primordial Germ Cells, Spermatogonial Stem Cells, Adult Germ Stem Cells, and Oocytes. In: Van Pham, P. (eds) Stem Cells: Biology and Engineering. Advances in Experimental Medicine and Biology(), vol 1083. Springer, Cham. https://doi.org/10.1007/5584_2017_117

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