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Detection and Viability Assessment of Endospore-Forming Pathogens

  • Chapter
Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems

Abstract

In this chapter, we explore technology developments for the rapid detection, identification, and viability assessment of endospore-forming pathogens with a focus on Bacillus anthracis. First, we introduce various toxin-producing species and their role as bioinsecticides, probiotics, and bioweapons. We also review the role of endospores as biological indicators (i.e., dosimeters) for evaluating sterilization regimens, such as autoclaving and wastewater remediation. Monitoring the effectiveness of cleaning and sterilization regimens to maintain good hygiene is required in several major industries, including health care, food, and pharmaceutical industries. In the next section, we review recent developments in DNA-, immuno-, and dipicolinic acid assays, and their applications for detection and monitoring of Bacillus anthracis and other endospore-forming pathogens. Finally, we review viability assays capable of rapid validation of endospore inactivation after sterilization, including assays based on ATP synthesis during stage II germination, and DPA release during stage I germination.

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Authors and Affiliations

  1. California Institute of Technology, 91125/Jet Propulsion Laboratory, Pasadena, CA 91109

    Adrian Ponce, Stephanie A. Connon & Pun To Yung

Authors
  1. Adrian Ponce
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  2. Stephanie A. Connon
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  3. Pun To Yung
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Editor information

Editors and Affiliations

  1. Biophage Pharma Inc, Montreal, Canada

    Mohammed Zourob

  2. Consultant to Biophage Pharma Inc, Montreal, Canada

    Souna Elwary

  3. Cranfield University, Bedfordshire, UK

    Anthony Turner

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© 2008 Springer Science+Business Media, LLC

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Ponce, A., Connon, S.A., Yung, P.T. (2008). Detection and Viability Assessment of Endospore-Forming Pathogens. In: Zourob, M., Elwary, S., Turner, A. (eds) Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems. Springer, New York, NY. https://doi.org/10.1007/978-0-387-75113-9_19

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  • DOI: https://doi.org/10.1007/978-0-387-75113-9_19

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