Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Anonymous. (1993). Statistics. Vocabulary and symbols. Part 1: Probability and general statistical terms (ISO 3534-1:1993). Geneva, Switzerland: International Organization for Standardization.
Anonymous. (1994). Accuracy (trueness and precision) of measurement and results (ISO 5725-1:1994).Geneva, Switzerland: International Organization for Standardization.
Anonymous. (1999). Salmonella. Detection in foods (5th ed., NKML no. 71). Esbo, Finland: Nordic Committee on Food Analysis.
Anonymous. (2003a). Microbiology of food and animal feeding stuffs – Protocol for the validation of alternative methods (ISO 16140:2003). Geneva, Switzerland: International Organization for Standardization.
Anonymous. (2003b). Microbiology of food and animal feeding stuffs – Horizontal method for the detection of Salmonella (EN ISO 6579:2003).Geneva, Switzerland: International Organization for Standardization.
Anonymous. (2003c). Microbiology of food and animal feeding stuffs – Preparation of test samples, initial suspension and decimal dilution for microbiological examination – Part 2: Specific rules for the preparation of meat and meat products (ISO 6887-2:2003). Geneva, Switzerland: International Organization for Standardization.
Anonymous. (2005a). Microbiology of food and animal feeding stuffs – Polymerase chain reaction (PCR) for the detection of food-borne pathogens – Performance testing for thermal cyclers (ISO/TS 20836:2005). Geneva, Switzerland: International Organization for Standardization.
Anonymous. (2005b). Microbiology of food and animal feeding stuffs – Polymerase chain reaction (PCR) for the detection of food-borne pathogens – General requirements and definitions (ISO 22174:2005). Geneva, Switzerland: International Organization for Standardization.
Anonymous. (2006a). Microbiology of food and animal feeding stuffs – Polymerase chain reaction (PCR) for the detection of food-borne pathogens – Requirements for sample preparation for qualitative detection (ISO 20837:2006). Geneva, Switzerland: International Organization for Standardization.
Anonymous. (2006b). Microbiology of food and animal feeding stuffs – Polymerase chain reaction (PCR) for the detection of food-borne pathogens – Requirements for amplification and detection for qualitative methods (ISO 20838:2006). Geneva, Switzerland: International Organization for Standardization.
Bohaychuk, V. M., Gensler, G. E., McFall, M. E., King, R. K., & Renter, D. G. (2007). A real-time PCR assay for the detection of Salmonella in a wide variety of food and food-animal matrices. Journal of Food Protection, 70, 1080–1087.
Cook, N. (2003). The use of NASBA for the detection of microbial pathogens in food and environmental samples. Journal of Microbiological Methods, 53, 165–174.
D’Agostino, M., & Rodríguez-Lázaro, D. (2009). Harmonisation and Validation of Methods in Food Safety - “FOOD-PCR”, a case study. In Barbosa-Cánovas, G., Mortimer, A., Colonna, P., Lineback, D., Spiess, W., Buckle, K. (Eds.), Global issues in food science and technology. Amsterdam: Elsevier.
Fach, P., Perelle, S., Dilasser, F., & Grout, J. (2001). Comparison between a PCR-ELISA test and the vero cell assay for detecting Shiga toxin-producing Escherichia coli in dairy products and characterization of virulence traits of the isolated strains. Journal of Applied Microbiology, 90, 809–818.
Heid, C. A., Stevens, J., Livak, K. J., & Williams, P. M. (1996). Real time quantitative PCR. Genome Research, 6, 86–94.
Hoorfar, J., & Cook, N. (2003). Critical aspects of standardization of PCR. Methods in Molecular Biology, 216, 51–64.
Hoorfar, J., Cook, N., Malorny, B., Wagner, M., De Medici, D., Abdulmawjood, A., et al. (2003). Making internal amplification control mandatory for diagnostic PCR. Journal of Clinical Microbiology, 41, 5835.
Hoorfar, J., Malorny, B., Abdulmawjood, A., Cook, N., Wagner, M., & Fach, P. (2004). Practical considerations in design of internal amplification controls for diagnostic PCR assays. Journal of Clinical Microbiology, 42, 1863–1868.
Josefsen, M. H., Jacobsen, N. R., & Hoorfar, J. (2004). Enrichment followed by quantitative PCR both for rapid detection and as a tool for quantitative risk assessment of food-borne thermotolerant campylobacters. Applied and Environmental Microbiology, 70, 3588–3592.
Josefsen, M. H., Krause, M., Hansen, F., & Hoorfar, J. (2007). Optimization of a 12-hour TaqMan PCR-based method for detection of Salmonella bacteria in meat. Applied and Environmental Microbiology, 73, 3040–3048.
Krause, M., Josefsen, M. H., Lund, M., Jacobsen, N. R., Brorsen, L., Moos, M., et al. (2006). Comparative, collaborative, and on-site validation of a TaqMan PCR method as a tool for certified production of fresh, campylobacter-free chickens. Applied and Environmental Microbiology, 72, 5463–5468.
Lübeck, P. S. L., Cook, N., Wagner, M., Fach, P., & Hoorfar, J. (2003). Toward an international standard for PCR-based detection of food-borne thermotolerant campylobacters. Part 2. Validation of the PCR assay in a multicenter collaborative trial. Applied and Environmental Microbiology, 69, 5670–5672.
Lübeck, P. S. L., Wolffs, P., On, S. L. W., Ahrens, P., Rådström, P., & Hoorfar, J. (2003). Toward an international standard for PCR-based detection of food-borne thermotolerant campylobacters. Part 1. Assay development and analytical validation. Applied and Environmental Microbiology, 69, 5664–5669.
Malorny, B., Bunge, C., & Helmuth, R. (2007). A real-time PCR for the detection of Salmonella Enteritidis in poultry meat and consumption eggs. Journal of Microbiological Methods, 70, 245–251.
Malorny, B., Hoorfar, J., Bunge, C., & Helmuth, R. (2003). Multicenter validation of the analytical accuracy of Salmonella PCR: Towards and international standard. Applied and Environmental Microbiology, 69, 290–296.
Malorny, B., Hoorfar, J., Hugas, M., Heuvelink, A., Fach, P., Ellerbroek, L., et al. (2003). Interlaboratory diagnostic accuracy of a Salmonella-specific PCR-based method. International Journal of Food Microbiology, 89, 241–249.
Malorny, B., Paccassoni, E., Fach, P., Bunge, C., Martin, A., & Helmuth, R. (2004). Diagnostic real-time PCR for detection of Salmonella in food. Applied and Environmental Microbiology, 70, 7046–7052.
Malorny, B., Tassios, P. T., Rådström, P., Cook, N., Wagner, M., & Hoorfar, J. (2003b). Standardization of diagnostic PCR for the detection of foodborne pathogens. International Journal of Food Microbiology, 83, 39–48.
Mengaud, J., Vicente, M. F., Chenevert, J., Pereira, J. M., Geoffroy, C., Gicquel-Sanzey, B., et al. (1988). Expression in Escherichia coli and sequence analysis of the listeriolysin O determinant of Listeria monocytogenes. Infection and Immunity, 56, 766–772.
Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., et al. (2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids Research, 28, E63.
Notzon, A., Helmuth, R., & Bauer, J. (2006). Evaluation of an immunomagnetic separation – Real-time PCR assay for the rapid detection of Salmonella in meat. Journal of Food Protection, 69, 2896–2901.
O’Grady, J., Sedano-Balbas, S., Maher, M., Smith, T., & Barrya, T. (2008). Rapid real-time PCR detection of Listeria monocytogenes in enriched food samples based on the ssrA gene, a novel diagnostic target. Food Microbiology, 25, 75–84.
Paoletti, C., & Wighardt, F. (2002). Definition of pre-validation performance requirements. 4th meeting of the European Network of GMO Laboratories – ENGL, 29–30 April, 2002. Ispra, Italy: Joint Research Centre.
Perelle, S., Dilasser, F., Grout, J., & Fach, P. (2002). Identification of the O-antigen biosynthesis genes of Escherichia coli O91 and development of a O91 PCR serotyping test. Journal of Applied Microbiology, 93, 758–764.
Perelle, S., Dilasser, F., Grout, J., & Fach, P. (2003). Development of a 5′-nuclease PCR assay for detecting Shiga toxin-producing Escherichia coli O145 based on the identification of an ‘O island 29’ homologue. Journal of Applied Microbiology, 94, 587–594.
Perelle, S., Dilasser, F., Grout, J., & Fach, P. (2004). Detection by 5′-nuclease PCR of Shiga-toxin producing Escherichia coli O26, O55, O91, O103, O111, O113, O145 and O157:H7, associated with the world’s most frequent clinical cases. Molecular and Cellular Probes, 18, 185–192.
Perelle, S., Dilasser, F., Grout, J., & Fach, P. (2007). Screening food raw materials for the presence of the world’s most frequent clinical cases of Shiga toxin-encoding Escherichia coli O26, O103, O111, O145 and O157. International Journal of Food Microbiology, 113, 284–288.
Perelle, S., Dilasser, F., Malorny, B., Grout, J., Hoorfar, J., & Fach, P. (2004). Comparison of PCR-ELISA and LightCycler real-time PCR assays for detecting Salmonella spp. in milk and meat samples. Molecular and Cellular Probes, 18, 409–420.
Perelle, S., Josefsen, M., Hoorfar, J., Dilasser, F., Grout, J., & Fach, P. (2004). A LightCycler real-time PCR hybridization probe assay for detecting food-borne thermophilic Campylobacter. Molecular and Cellular Probes, 18, 321–327.
Rådström, P., Knutsson, R., Wolffs, P., Dahlenborg, M., & Löfström, C. (2003). Pre-PCR processing of samples. Methods in Molecular Biology, 216, 31–50.
Rodriguez-Lazaro, D., & Hernandez, M. (2006). Isolation of Listeria monocytogenes DNA from meat products for quantitative detection by real-time PCR. Journal of Rapid Methods and Automation in Microbiology, 14, 395–404.
Rodriguez-Lazaro, D., Hernandez, M., D’Agostino, M., & Cook, N. (2006). Application of nucleic acid sequence-based amplification for the detection of viable foodborne pathogens: Progress and challenges. Journal of Rapid Methods and Automation in Microbiology, 14, 218–236.
Rodriguez-Lazaro, D., Hernandez, M., Scortti, M., Esteve, T., Vazquez-Boland, J. A., & Pla, M. (2004). Quantitative detection of Listeria monocytogenes and Listeria innocua by real-time PCR: Assessment of hly, iap, and lin02483 targets and AmpliFluor technology. Applied and Environmental Microbiology, 70, 1366–1377.
Rodriguez-Lazaro, D., Jofre, A., Aymerich, T., Hugas, M., & Pla, M. (2004). Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR. Applied and Environmental Microbiology, 70, 6299–6301.
Rodriguez-Lazaro, D., Lombard, B., Smith, H., Rzezutka, A., D’Agostino, M., Helmuth, R., et al. (2007). Trends in analytical methodology in food safety and quality: Monitoring microorganisms and genetically modified organisms. Trends in Food Science and Technology, 18, 306–319.
Rodriguez-Lazaro, D., Pla, M., Scortti, M., Monzo, H. J., & Vazquez-Boland, J. A. (2005). A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control. Applied and Environmental Microbiology, 71, 9008–9012.
Rossen, L., Nøskov, P., Holmstrøm, K., & Rasmussen, O. F. (1992). Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA extraction solution. International Journal of Food Microbiology, 17, 37–45.
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., et al. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239, 487–491.
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., et al. (1985). Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, 230, 1350–1354.
Scheu, P. M., Berghof, K., & Stahl, U. (1998). Detection of pathogenic and spoilage microorganisms in food with the polymerase chain reaction. Food Microbiology, 15, 13–31.
Sharma, V. K. (2002). Detection and quantitation of enterohemorrhagic Escherichia coli O157, O111, and O26 in beef and bovine feces by real-time polymerase chain reaction. Journal of Food Protection, 65, 1371–1380.
Skoog, D. A., & Leary, J. J. (1992). Principles of instrumental analysis. London, UK: Saunders College Publishing.
Stefan, A., Scaramagli, S., Bergami, R., Mazzini, C., Barbanera, M., Perelle, S., et al. (2007). Real-time PCR and enzyme-linked fluorescent assay methods for detecting Shiga-toxin-producing Escherichia coli in mincemeat samples. Canadian Journal of Microbiology, 53, 337–342.
Thompson, M., Ellison, S. L. R., & Wood, R. (2002). Harmonised guidelines for single-laboratory validation of methods of analysis (IUAPC Technical Report). Pure and Applied Chemistry, 74, 835–855.
Walker, J. A., Hughes, D. A., Anders, B. A., Shewale, J., Sinha, S. K., & Batzer, M. A. (2003). Quantitative intra-short interspersed element PCR for species-specific DNA identification. Analytical Biochemistry, 316, 259–269.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer Science+Business Media, LLC
About this chapter
Cite this chapter
Hernández, M., Hansen, F., Cook, N., Rodríguez-Lázaro, D. (2009). Real-Time PCR Methods for Detection of Foodborne Bacterial Pathogens in Meat and Meat Products. In: Toldrá, F. (eds) Safety of Meat and Processed Meat. Food Microbiology and Food Safety. Springer, New York, NY. https://doi.org/10.1007/978-0-387-89026-5_16
Download citation
DOI: https://doi.org/10.1007/978-0-387-89026-5_16
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-0-387-89025-8
Online ISBN: 978-0-387-89026-5
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)