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The Use of Organic Dyes in the Elucidation of Enzyme Structures and Mechanisms

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Reactive Dyes in Protein and Enzyme Technology

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Abstract

One of the most fundamental problems of enzymology is the elucidation of the molecular mechanisms by which enzymes work. The determination of the way in which such complex structures act to bring about efficient, specific catalytic reactions is a very interesting but difficult problem which can only be solved by the assembly of evidence from many different lines of research. Many factors contribute to the extraordinary catalytic properties of enzymes, including the precise juxtapositions of functional groups and particularly favourable micro-environments at active sites which enable specific reactions to occur. Precisely these factors influence the reactivity of functional groups in chemical modification and affinity labelling studies of enzymes.

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Authors
  1. J. E. C. McArdell
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  2. C. J. Bruton
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Editor information

Editors and Affiliations

  1. The Biotechnology Centre, University of Cambridge, Downing Street, Cambridge, UK

    Y. D. Clonis  & C. R. Lowe  & 

  2. Microbial Technology Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK

    T. Atkinson

  3. Parke Davis Research Unit, Addenbrooke’s Hospital Site, Hills Road, Cambridge, UK

    C. J. Bruton

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McArdell, J.E.C., Bruton, C.J. (1987). The Use of Organic Dyes in the Elucidation of Enzyme Structures and Mechanisms. In: Clonis, Y.D., Atkinson, T., Bruton, C.J., Lowe, C.R. (eds) Reactive Dyes in Protein and Enzyme Technology. Palgrave, London. https://doi.org/10.1007/978-1-349-06582-0_8

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