Since there are still no chemicals that can be applied routinely to control plant virus diseases, TYLCV control strategies have been mainly focused on methods to prevent the occurrence of infection and on genetic resistance. Attempts to reduce the incidence of TYLCV by eliminating the sources of inoculum or controlling vector transmission are often ineffective (Picó et al., 1996). Attempts to derive TYLCV resistant tomato cultivars constituted the main effort of extended breeding programmes to introgress resistance from wild Lycopersicon species. Although some wild relatives of tomato are resistant, introduction of resistance traits into commercial tomatoes is however complicated by several factors. Some tolerant cultivars have been released (Lapidot et al., 1997; Friedmann et al., 1998), but no fully resistants Lycopersicon esculentum are still available.
The identification of plant genes involved in the viral life cycle may offer the opportunity to disrupt the interaction between the virus and the plant cell, thus preventing infection without introducing foreign genes in the plant. Despite differences in the properties of their genomes, all plant viruses face the same two fundamental challenges during the establishment of systemic infections in their plant hosts. The first necessity is to replicate in the infected cells. The second requirement is to move through adjacent plant cells to the vascular system, before spreading throughout the plant. Both processes depend on highly specific interactions with host proteins. Protein-protein interactions are the underpinnings of a vast number of these cellular processes. In recent years, the convergence of biochemistry, cellular and molecular biology has made available a number of powerful techniques for studying such interactions. These techniques vary in their sensitivity, efficiency and rapidity, but judicial deployment of a combination of them has proved to be effective and reliable.
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References
Ach, R. A., Durfee, T., Miller, A. B., Taranto, P., Hanley-Bowdoin, L., Zambryski, P. C., & Gruissem, W. (1997). RRB1 and RRB2 encode maize retinoblastoma-related proteins that interact with a plant D-type cyclin and geminivirus replication protein. Mol. Cell Biol. 17, 5077–5086.
Arguello-Astorga, G., Lopez-Ochoa, L., Kong, L. J., Orozco, B. M., Settlage, S. B., & Hanley-Bowdoin, L. (2004). A novel motif in geminivirus replication proteins interacts with the plant retinoblastoma-related protein. J. Virol. 78, 4817–4826.
Bagewadi, B., Chen, S., Lal, S. K., Choudhury, N. R., & Mukherjee, S. K. (2004). PCNA interacts with Indian mung bean yellow mosaic virus rep and downregulates Rep activity. J. Virol. 78, 11890–11903.
Boggio, R. & Chiocca, S. (2006). Viruses and sumoylation: recent highlights. Curr. Opin. Microbiol. 9, 430–436.
Bossis, G. & Melchior, F. (2006). SUMO: regulating the regulator. Cell Div. 1, 13.
Castillo, A. G., Collinet, D., Deret, S., Kashoggi, A., & Bejarano, E. R. (2003). Dual interaction of plant PCNA with geminivirus replication accessory protein (Ren) and viral replication protein (Rep). Virology 312, 381–394.
Castillo, A. G., Kong, L. J., Hanley-Bowdoin, L., & Bejarano, E. R. (2004). Interaction between a geminivirus replication protein and the plant sumoylation system. J. Virol. 78, 2758–2769.
Cruz, S. S., Chapman, S., Roberts, A. G., Roberts, I. M., Prior, D. A., & Oparka, K. J. (1996). Assembly and movement of a plant virus carrying a green fluorescent protein overcoat. Proc. Natl. Acad. Sci. USA 93, 6286–6290.
Fields, S. (2005). High-throughput two-hybrid analysis (The promise and the peril). FEBS J. 272, 5391–5399.
Friedmann, M., Lapidot, M., Cohen, S., & Pilowsky, M. (1998). A novel source of resistance to tomato yellow leaf curl virus (TYLCV) exhibiting a symptomless reaction to viral infection. J. Am. Soc. Hortic. Sci. 123, 1004–1006.
Gietz, R. D. (2006). Yeast two-hybrid system screening. Methods Mol. Biol. 313, 345–371.
Gilbertson, R. L., Sudarshana, M., Jiang, H., Rojas, M. R., & Lucas, W. J. (2003). Limitations on geminivirus genome size imposed by plasmodesmata and virus-encoded movement protein: insights into DNA trafficking. Plant Cell 15, 2578–2591.
Gurlebeck, D., Thieme, F., & Bonas, U. (2006). Type III effector proteins from the plant pathogen Xanthomonas and their role in the interaction with the host plant. J. Plant Physiol. 163, 233–255.
Gutierrez, C., Ramirez-Parra, E., Mar Castellano, M., Sanz-Burgos, A. P., Luque, A., & Mikssich, R. (2004). Geminivirus DNA replication and cell cycle interactions. Vet. Microbiol. 98, 111–119.
Hanania, U., Furman-Matarasso, N., Ron, M., & Avni, A. (1999). Isolation of a novel SUMO protein from tomato that suppresses EIX-induced cell death. Plant J. 19, 533–541.
Hanley-Bowdoin, L., Settlage, S. B., Orozco, B. M., Nagar, S., & Robertson, D. (2000). Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Crit. Rev. Biochem. Mol. Biol. 35, 105–140.
Hanley-Bowdoin, L., Settlage, S. B., & Robertson, D. (2004). Reprogramming plant gene expression: a prerequisite to geminivirus DNA replication. Mol. Plant Pathol. 5.
Hay, R. (2005). SUMO: a history of modification. Mol. Cell 18, 1–12.
Hayes, R. J., Coutts, R. H., & Buck, K. W. (1989). Stability and expression of bacterial genes in replicating geminivirus vectors in plants. Nucleic Acids Res. 17, 2391–2403.
Hefferon, K. L., Kipp, P., & Moon, Y. S. (2004). Expression and purification of heterologous proteins in plant tissue using a geminivirus vector system. J. Mol. Microbiol. Biotechnol. 7, 109–114.
Hong, Y., Stanley, J., & van Wezel, R. (2003). Novel system for the simultaneous analysis of geminivirus DNA replication and plant interactions in Nicotiana benthamiana. J. Virol. 77, 13315–13322.
Kelman, Z. (1997). PCNA: structure, functions and interactions. Oncogene 14, 629–640.
Kong, L. J. & Hanley-Bowdoin, L. (2002). A geminivirus replication protein interacts with a protein kinase and a motor protein that display different expression patterns during plant development and infection. Plant Cell 14, 1817–1832.
Lapidot, M., Friedmann, M., Lachman, O., Yehezkel, A., Nahon, S., Cohen, S., & Pilowsky, M. (1997). Comparison of resistance yo tomato yellow leaf curl virus among commercial cultivars and breeding lines. Plant Dis. 81, 1425–1428.
Laufs, J., Schumacher, S., Geisler, N., Jupin, I., & Gronenborn, B. (1995). Identification of the nicking tyrosine of geminivirus Rep protein. FEBS Lett. 377, 258–262.
Lee, L. K., & Roth, C. M. (2003). Antisense technology in molecular and cellular bioengineering. Curr. Opin. Biotechnol. 14, 505–511.
Levy, A., & Czosnek, H. (2003). The DNA-B of the non-phloem-limited bean dwarf mosaic virus (BDMV) is able to move the phloem-limited Abutilon mosaic virus (AbMV) out of the phloem, but DNA-B of AbMV is unable to confine BDMV to the phloem. Plant Mol. Biol. 53, 789–803.
Lu, R., Martin-Hernandez, A. M., Peart, J. R., Malcuit, I., & Baulcombe, D. C. (2003). Virus-induced gene silencing in plants. Methods 30, 296–303.
Lucioli, A., Noris, E., Brunetti, A., Tavazza, R., Ruzza, V., Castillo, A. G., Bejarano, E. R., Accotto, G. P., & Tavazza, M. (2003). Tomato yellow leaf curl Sardinia virus rep-derived resistance to homologous and heterologous geminiviruses occurs by different mechanisms and is overcome if virus-mediated transgene silencing is activated. J. Virol. 77, 6785–6798.
Luque, A., Sanz-Burgos, A. P., Ramirez-Parra, E., Castellano, M. M., & Gutierrez, C. (2002). Interaction of geminivirus Rep protein with replication factor C and its potential role during geminivirus DNA replication. Virology 302, 83–94.
Mor, T. S., Moon, Y. S., Palmer, K. E., & Mason, H. S. (2002). Geminivirus vectors for high-level expression of foreign proteins in plant cells. Biotechnol. Bioeng. 81, 430–437.
Morilla, G., Castillo, A. G., Preiss, W., Jeske, H., & Bejarano, E. R. (2006). A versatile transreplicationbased system to identify cellular proteins involved in geminivirus replication. J. Virol. 80, 3624–3633.
Muller, S., Hoege, C., Pyrowolakis, G., & Jentsch, S. (2001). SUMO, ubiquitin’s mysterious cousin. Nat. Rev. Mol. Cell Biol. 2, 202–210.
Novatchkova, M., Budhiraja, R., Coupland, G., Eisenhaber, F., & Bachmair, A. (2004). SUMO conjugation in plants. Planta 220, 1–8.
Palmer, K. E., Thomson, J. A., & Rybicki, E. P. (1999). Generation of maize cell lines containing autonomously replicating maize streak virus-based gene vectors. Arch. Virol. 144, 1345–1360.
Peart, J. R., Cook, G., Feys, B. J., Parker, J. E., & Baulcombe, D. C. (2002). An EDS1 orthologue is required for N-mediated resistance against tobacco mosaic virus. Plant J. 29, 569–579.
Peele, C., Jordan, C. V., Muangsan, N., Turnage, M., Egelkrout, E., Eagle, P., Hanley-Bowdoin, L., & Robertson, D. (2001). Silencing of a meristematic gene using geminivirus-derived vectors. Plant J. 27, 357–366.
Picó, B., Diez, M. J., & Nuez, F. (1996). Viral diseases causing the greatest economic losses to the tomato crop. II. The tomato yellow leaf curl Virus–a review. Sci. Horti. 6751–196.
Ratcliff, F., Martin-Hernandez, A. M., & Baulcombe, D. (2001). Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J. 25, 237–245.
Selth, L. A., Dogra, S. C., Rasheed, M. S., Healy, H., Randles, J. W., & Rezaian, M. A. (2005). A NAC domain protein interacts with tomato leaf curl virus replication accessory protein and enhances viral replication. Plant Cell 17, 311–325.
Settlage, S. B., See, R. G., & Hanley-Bowdoin, L. (2005). Geminivirus C3 protein: replication enhancement and protein interactions. J Virol. 79, 9885–9895.
Sudarshana, M. R., Wang, H. L., Lucas, W. J., & Gilbertson, R. L. (1998). Dynamics of bean dwarf mosaic geminivirus cell-to-cell and long-distance movement in Phaseolus vulgaris revealed, using the green fluorescent protein. Mol. Plant Microbe Interact. 11, 277–291.
Timmermans, M. C., Das, O. P., & Messing, J. (1992). Trans replication and high copy numbers of wheat dwarf virus vectors in maize cells. Nucleic Acids Res. 20, 4047–4054.
Tsurimoto, T. (1999). PCNA binding proteins. Front Biosci. 4, D849–D858.
Warbrick, E. (2000). The puzzle of PCNA’s many partners. Bioessays 22, 997–1006.
Yeh, E. T., Gong, L., & Kamitani, T. (2000). Ubiquitin-like proteins: new wines in new bottles. Gene 248, 1–14.
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Castillo, A.G. et al. (2007). Identification of Plant Genes Involved in TYLCV Replication. In: Czosnek, H. (eds) Tomato Yellow Leaf Curl Virus Disease. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-4769-5_12
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