Abstract
This work aims to investigate the changes occurring in metabolism of a cell population during adaptation of a cell line from serum containing to serum free media. In order to enable growth in a serum free media a supplement was added to DME:F12. Cells grown in 1.25% serum media were used to investigate the effect of the supplement, data for growth, antibody production and key metabolites are presented All data presented here are derived from cells banked at different serum concentrations during the adaptation and subsequently grown in 50mL culture in 250mL shaken ehrlenmyer flasks.
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© 2007 Springer
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Denby, S., Baganz, F., Mousdale, D. (2007). Metabolic Changes Occuring During Adaptation to Serum Free Media. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_81
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DOI: https://doi.org/10.1007/978-1-4020-5476-1_81
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
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