Skip to main content
SpringerLink
Log in

Embryogenesis In Vitro

  • Conference paper
Biotechnology and Sustainable Agriculture 2006 and Beyond

This review will consider several aspects of the induction and exploitation of in vitro embryogenesis in plants. In particular it will describe recent molecular data that has supplemented the earlier empirical information obtained largely by trial and error. It will also describe how the process of induced embryogenesis is commercially exploited.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Boutilier K, Offringa R, Sharma VK, Kieft H, Ouellet T, Zhang L, Hattori J, Liu CM, Van Lammeren AA, Miki BL, Custers JB, Van Lookeren Campagne MM (2002) Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth. Plant Cell 14: 1737-1749.

    Article  CAS  PubMed  Google Scholar 

  • Bozhkov PV, Filonova LH, Suarez MF (2005a) Programmed cell death in plant embryogenesis. Curr Top Dev Biol 67: 135-179.

    Article  CAS  PubMed  Google Scholar 

  • Bozhkov PV, Suarez MF, Filonova LH, Daniel G, Zamyatnin AA, Rodriguez-Nieto S, Zhivotovsky B, Smertenko A (2005b) Cysteine protease mcII-Pa executes programmed cell death during plant embryogenesis. Proc Natl Acad Sci USA 102: 14463-14468.

    Article  CAS  PubMed  Google Scholar 

  • Braybrook SA, Stone SL, Park S, Bui AQ, Le BH, Fischer RL, Goldberg RB, Harada JJ (2006) Genes directly regulated by LEAFY COTYLEDON2 provide insight into the control of embryo maturation and somatic embryogenesis. Proc Natl Acad Sci USA 103: 3468-3473.

    Article  CAS  PubMed  Google Scholar 

  • Che P, Love TM, Frame BR, Wang K, Carriquiry AL, Howell SH (2006) Gene expression patterns during somatic embryo development and germination in maize Hi II callus cultures. Plant Mol Biol 62: 1-14.

    Article  CAS  PubMed  Google Scholar 

  • Ding YH, Liu NY, Tang ZS, Liu J, Yang WC (2006) Arabidopsis GLUTAMINE-RICH PROTEIN23 is essential for early embryogenesis and encodes a novel nuclear PPR motif protein that interacts with RNA polymerase II subunit III. Plant Cell 18: 815-830.

    Article  CAS  PubMed  Google Scholar 

  • Dunwell JM (1976) A comparative study of environmental and developmental factors which influence embryo induction and growth in cultured anthers of Nicotiana tabacum. Environ Exp Bot 16: 109-118.

    Article  Google Scholar 

  • Dunwell JM (1978) Division and differentiation in cultured pollen. In: Thorpe TA (ed) Frontiers of Plant Tissue Culture 1978. Int Assoc Plant Tissue Cult. Cal-gary, pp. 103-112.

    Google Scholar 

  • Dunwell JM (1985) Embryogenesis from pollen in vitro. In: Zaitlin P, Day P, Hollaender A (eds) Biotechnology in Plant Science. Academic Press, Orlando, pp. 49-76.

    Google Scholar 

  • Dunwell JM (1992) Mechanisms of microspore embryogenesis. In: Dattée Y, Dumas C, Gallais A (eds) Reproductive Biology and Plant Breeding. Springer-Verlag, Berlin, pp. 121-130.

    Google Scholar 

  • Dunwell JM (2005) Review: Intellectual property aspects of plant transformation. Plant Biotech J 3: 371-384.

    Article  CAS  Google Scholar 

  • Farlow WG (1874) An asexual growth from the prothallus of Pteris cretica. Q J Microsc Sci 14: 266-272.

    Google Scholar 

  • Gaj MD, Zhang S, Harada JJ, Lemaux PG (2005) Leafy cotyledon genes are essential for induction of somatic embryogenesis of Arabidopsis. Planta 222: 977-988.

    Article  CAS  PubMed  Google Scholar 

  • Guha S, Maheshwari SC (1964). In vitro production of embryos from anthers of Datura. Nature 204: 497.

    Article  Google Scholar 

  • Hirsch AM (1975) The effect of sucrose on the differentiation of excised fern leaf tissue into either gametophytes or sporophytes. Plant Physiol 56: 390-393.

    Article  CAS  PubMed  Google Scholar 

  • Hosp J, Tashpulatov A, Roessner U, Barsova E, Katholnigg H, Steinborn R, Melikant B, Lukyanov S, Heberle-Bors E, Touraev A (2007) Transcriptional and metabolic profiles of stress-induced, embryogenic tobacco microspores. Plant Mol Biol 63: 137-149.

    Article  CAS  PubMed  Google Scholar 

  • Jenik PD, Barton MK (2005) Surge and destroy: the role of auxin in plant embryogenesis. Development 132: 3577-3585.

    Article  CAS  PubMed  Google Scholar 

  • Jenik PD, Jurkuta RE, Barton MK (2005) Interactions between the cell cycle and embryonic patterning in Arabidopsis uncovered by a mutation in DNA poly-merase epsilon. Plant Cell 17: 3362-3377.

    Article  CAS  PubMed  Google Scholar 

  • Kang TJ, Lee WS, Choi EG, Kim JW, Kim BG, Yang MS (2006) Mass produc-tion of somatic embryos expressing Escherichia coli heat-labile enterotoxin B subunit in Siberian ginseng. J Biotechnol 121: 124-133.

    Article  CAS  PubMed  Google Scholar 

  • Kim I, Zambryski PC (2005) Cell-to-cell communication via plasmodesmata dur-ing Arabidopsis embryogenesis. Curr Opin Plant Biol 8: 593-599.

    Article  CAS  PubMed  Google Scholar 

  • Lakshmanan P, Geijskes RJ, Wang L, Elliott A, Grof CP, Berding N, Smith GR (2006) Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane (Saccharum spp. interspecific hybrids) leaf culture. Plant Cell Rep 25: 1007-1015.

    Article  CAS  PubMed  Google Scholar 

  • Latvala-Kilby SM, Kilby NJ (2006) Uncovering the post-embryonic role of embryo essential genes in Arabidopsis using the controlled induction of visibly marked genetic mosaics: EMB506, an illustration. Plant Mol Biol 61:179-184.

    Article  CAS  PubMed  Google Scholar 

  • Le Q, Gutierrez-Marcos JF, Costa LM, Meyer S, Dickinson HG, Lorz H, Kranz E, Scholten S (2005) Construction and screening of subtracted cDNA libraries from limited populations of plant cells: A comparative analysis of gene expression between maize egg cells and central cells. Plant J 44: 167-178.

    Article  CAS  PubMed  Google Scholar 

  • Leibfried A, To JP, Busch W, Stehling S, Kehle A, Demar M, Kieber JJ, Lohmann JU (2005) WUSCHEL controls meristem function by direct regulation of cytokinin-inducible response regulators. Nature 438: 1172-1175.

    Article  CAS  PubMed  Google Scholar 

  • Long JA, Ohno C, Smith ZR, Meyerowitz EM (2006) TOPLESS regulates apical embryonic fate in Arabidopsis. Science 312: 1520-1523.

    Article  CAS  PubMed  Google Scholar 

  • Malik MR, Wang F, Dirpaul JM, Zhou N, Polowick PL, Ferrie AM, Krochko JE (2007) Transcript profiling and identification of molecular markers for early microspore embryogenesis in Brassica napus. Plant Physiol 144: 134-154.

    Article  CAS  PubMed  Google Scholar 

  • Nehra N, Becwar MR, Rottmann WH, Pearson L, Chowdhury K, Chang S, Wilde HD, Kodrzycki RJ, Zhang C, Gause KC, Parks DW, Hinchee MA (2005) Forest biotechnology: Innovative methods, emerging opportunities. In Vitro Cell Dev Biol - Plant 41: 701-717.

    Article  CAS  Google Scholar 

  • Ning J, Peng XB, Qu LH, Xin HP, Yan TT, Sun M. (2006) Differential gene ex-pression in egg cells and zygotes suggests that the transcriptome is restricted before the first zygotic division in tobacco. FEBS Lett 580: 1747-1752.

    Article  CAS  PubMed  Google Scholar 

  • Nolan KE, Saeed NA, Rose RJ. (2006) The stress kinase gene MtSK1 in Medi-cago truncatula with particular reference to somatic embryogenesis. Plant Cell Rep 25: 711-722.

    Article  CAS  PubMed  Google Scholar 

  • Okamoto T, Scholten S, Lorz H, Kranz E. (2005) Identification of genes that are up- or down-regulated in the apical or basal cell of maize two-celled embryos and monitoring their expression during zygote development by a cell manipu-lation- and PCR-based approach. Plant Cell Physiol 46: 332-338.

    Article  CAS  PubMed  Google Scholar 

  • Palmer CED, Keller WA (2005) Challenges and limitations to the use of haploidy in crop improvement. In: Palmer CED, Keller WA, Kasha KJ (eds) Haploids in Crop Improvement II. Springer, Berlin Heidelberg. pp. 295-303.

    Chapter  Google Scholar 

  • Quint M, Gray WM (2006) Auxin signaling. Curr Opin Plant Biol 9: 448-453.

    Article  CAS  Google Scholar 

  • Segui-Simarro JM, Barany I, Suarez R, Fadon B, Testillano PS, Risueno MC. (2006) Nuclear bodies domain changes with microspore reprogramming to embryogenesis. Eur J Histochem 50: 35-44.

    CAS  PubMed  Google Scholar 

  • Shariatpanahi ME, Belogradova K, Hessamvaziri L, Heberle-Bors E, Touraev A (2006) Efficient embryogenesis and regeneration in freshly isolated and cul-tured wheat (Triticum aestivum L.) microspores without stress pretreatment. Plant Cell Rep 25: 1294-1299.

    Article  CAS  PubMed  Google Scholar 

  • Sunderland N, Dunwell JM (1974) Pathways in pollen embryogenesis. In: Street HE (ed) Tissue Culture and Plant Science 1974. Academic Press, London, pp. 141-167.

    Google Scholar 

  • Tang XC, He YQ, Wang Y, Sun MX (2006) The role of arabinogalactan proteins binding to Yariv reagents in the initiation, cell developmental fate, and main-tenance of microspore embryogenesis in Brassica napus L. cv. Topas. J Exp Bot 57: 2639-2650.

    Article  CAS  Google Scholar 

  • Wei J, Li XR, Sun MX (2006) Establishment of a simple and efficient system for somatic embryo induction via ovule culture in Arabidopsis thaliana. Plant Cell Rep 25: 1275-1280.

    Article  CAS  PubMed  Google Scholar 

  • Whittier DP, Steeves TA (1960) The induction of apogamy in the bracken fern. Can J Bot 38: 925-930.

    Article  CAS  Google Scholar 

  • Winkelmann T, Heintz D, Van Dorsselaer A, Serek M, Braun HP (2006) Proteo-mic analyses of somatic and zygotic embryos of Cyclamen persicum Mill. re-veal new insights into seed and germination physiology. Planta 224: 508-519.

    Article  CAS  PubMed  Google Scholar 

  • Xiao W, Custard KD, Brown RC, Lemmon BE, Harada JJ, Goldberg RB, Fischer RL (2006) DNA methylation is critical for Arabidopsis embryogenesis and seed viability. Plant Cell 18: 805-814.

    Article  CAS  PubMed  Google Scholar 

  • Xu J, Zhang HY, Xie CH, Xue HW, Dijkhuis P, Liu CM (2005) EMBRYONIC FACTOR 1 encodes an AMP deaminase and is essential for the zygote to em-bryo transition in Arabidopsis. Plant J 42: 743-756.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Biological Sciences, University of Reading, RG6 6AS, Whiteknights, Reading, UK

    Jim M. Dunwell

Authors
  1. Jim M. Dunwell
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Peking University, Beijing, China

    Zhihong Xu

  2. Chinese Academy of Sciences, Beijing, China

    Jiayang Li , Yongbiao Xue  & Weicai Yang ,  & 

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer

About this paper

Cite this paper

Dunwell, J.M. (2007). Embryogenesis In Vitro. In: Xu, Z., Li, J., Xue, Y., Yang, W. (eds) Biotechnology and Sustainable Agriculture 2006 and Beyond. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6635-1_3

Download citation

Publish with us

Policies and ethics

Search

Navigation