Skip to main content

Abstract

Application of chemicals to restrict the incidence and spread of different diseases affecting various crops has been the important and largely visible disease management strategy. With the advent of modern fungicides with single-site of action on target pathogens, emergence of resistant strains, has resulted in restricted use or withdrawal of such fungicides/chemicals, seriously affecting the fungicide market. Biochemical and nucleic acid-based molecular techniques have been shown to yield reliable and reproducible results rapidly, facilitating the planning of effective action schedules. The molecular methods can be used for detection, differentiation and quantification of sensitive and resistant strains/isolates of fungal and bacterial plant pathogens. In addition, the occurrence of exotic strains exhibiting resistance can be efficiently detected and contained by taking appropriate measures. The importance of continuous monitoring the populations of different isolates of pathogens coupled with resistance management activities for effective crop disease management is underscored.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Refrences

  • Albertini C, Leroux P (2004) A putative Botrytis cinerea 3-keto reductase gene (ERG27) that is homologous to the mammalian 17-hydroxysteroid dehydrogenase type 7 gene (17-HSD 7). Eur J Plant Pathol 110: 723–733

    Article  CAS  Google Scholar 

  • Anderson GL, Menkissoglou O, Lindow SE (1991) Occurrence and properties of copper-tolerant strains of Pseudomonas syringae isolated from fruit trees in California. Phytopathology 81: 648–656

    Article  Google Scholar 

  • Barlett DW, Clough JM, Godwin JR, Hall AA, Hamer M, Parr-Dobrzanski B (2002) The strobilurin fungicides. Pest Manag Sci 58: 649–662

    Article  CAS  Google Scholar 

  • Basim H, Minsavage GV, Stall RE, Wang JF, Shanker S, Jones JB (2005) Characterization of a unique chromosomal copper resistance gene cluster from Xanthomonas campestris pv. vesicatoria. Appl Environ Microbiol 71: 8284–8291

    Article  PubMed  CAS  Google Scholar 

  • Basim H, Stall RE, Minsavage GV, Jones JB (1999) Chromosomal gene transfer by conjugation in the plant pathogen Xanthomonas axonopodis pv. vesicatoria. Phytopathology 89: 1044–1049

    Article  CAS  Google Scholar 

  • Bender CL, Malvic DK, Conway KE, George S, Pratt P (1990) Characterization of pXV10A, a copper resistance plasmid in Xanthomonas campestris pv. vesicatoria. Appl Environ Microbiol 56: 170–175

    PubMed  CAS  Google Scholar 

  • Bhat RG, Colowit PM, Tai TH, Aradhya MK, Browne GT (2006) Genetic and pathogenic variation in Phytophthora cactorum affecting fruit and nut crops in California. Plant Dis 90: 161–169

    Article  CAS  Google Scholar 

  • Bhat RG, McBlain BA, Schmitthenner AF (1993) The inheritance of resistance to metalaxyl and to fluorophenylalanine in matings of homothallic Phytophthora sojae. Mycol Res 97: 865–870

    CAS  Google Scholar 

  • Chung WH, Ishii H, Nishimura K, Fukaya M, Yano K, Kajitani T (2006) Fungicide sensitivity and phylogenetic relationship of anthracnose fungi isolated from various fruit crops in Japan. Plant Dis 90: 506–512

    Article  CAS  Google Scholar 

  • Cooksey DA (1996) Molecular genetics and evolution of copper resistance in bacterial plant pathogens. In: Brown TM (ed) Molecular genetics and evolution of pesticide resistance, American Chemical Society, Washington DC, pp 79–88

    Google Scholar 

  • Cooksey DA, Azad HR, Cha JS, Lim CK (1990) Copper resistance gene homologs in pathogenic and saprophytic bacterial species from tomato. Appl Environ Microbiol 130: 2447–2455

    Google Scholar 

  • Cools HJ, Ishii H, Butters JA, Hollomon DW (2002) Cloning and sequence analysis of the eubricol 14 $\ualpha$-demethylase encoding gene (CYP51) from Japanese pear scab fungus Venturia nashicola. J Phytopathol 150: 444–450

    Article  CAS  Google Scholar 

  • Davidse LC, Ishii H (1995) Biochemical and molecular aspects of the mechanisms of action of benzimidazoles, N-phenylcarbamates, N-phenylformamidoximes and the mechanism of resistance to these compounds in fungi. In: Lyr H (ed) Modern selective fungicides, properties, application, mechanisms of action. Gustav Fisherverlag, New York, pp 305–322

    Google Scholar 

  • del Sorbo G, Schoonbeek HJ, de Waard MA (2000) Fungal transporters involved in the efflux of natural toxic compounds and fungicides. Fungal Genet Biol 30: 1–15

    Article  PubMed  CAS  Google Scholar 

  • Délye C, Laigret F, Corio-Costet MF (1997) A mutation in the 14 alpha-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor. Appl Environ Microbiol 63: 2966–2970

    PubMed  Google Scholar 

  • De Waard MA (1996) Molecular genetics of resistance in fungi to azole fungicides. ACS Symp Ser 645: 62–71

    Google Scholar 

  • Errampalli D, Saunders JM, Holley JD (2001) Emergence of silver scurf (Helminthosporium solani) as an economically important disease of potato. Plant Pathol 26: 70–75

    Google Scholar 

  • Fabritius AL, Shattock RC, Judelson HS (1997) Genetic analysis of metalaxyl insensitivity loci in Phytophthora infestans using linked DNA markers. Phytopathology 87: 1034–1040

    Article  CAS  PubMed  Google Scholar 

  • Gisi U, Sierotzki H, Cook A, McCaffery A (2002) Mechanisms influencing the evolution of resistance to QoI inhibitor fungicides. Pest Manag Sci 58: 859–867

    Article  PubMed  CAS  Google Scholar 

  • Gisi U, Waldner M, Kraus N, Dubuis PH, Sierotzki H (2007) Inheritance of resistance to carboxylic acid amide (CAA) fungicides in Plasmopara viticola. Plant Pathol 56: 199–208

    Article  CAS  Google Scholar 

  • Grasso V, Sierotzki H, Garibalidi A, Gisi U (2006) Characterization of the cytochrome $b$ gene fragment of Puccinia species responsible for the binding site of QoI fungicides. Pestic Biochem Physiol 84: 72–82

    Article  CAS  Google Scholar 

  • Grünwald NJ, Sturbaum AK, Montes GR, Serrano GE, Lozoya-Saldaña H, Fry WE (2006) Selection of fungicide resistance within a growing season in field populations of Phytophthora infestans at the center of origin. Phytopathology 96: 1397–1403

    Article  CAS  PubMed  Google Scholar 

  • Hamamoto H, Haseqawa K, Nakaune R, Lee YJ, Akustsa K, Hibi T (2001) PCR-based detection of sterol demethylation inhibitor-resistant strains of Penicillium digitatum. Pest Manage Sci 57: 839–843

    Article  CAS  Google Scholar 

  • Hamamoto H, Hasegawa K, Nakaune R, Lee YJ, Makizumi Y, Akutsu K, Hibi T (2000) Tandem repeat of a transcriptional enhancer upstream of the sterol 14 $\ualpha$-demethylase gene (CYP51) in Penicillium digitatum. Appl Environ Microbiol 66: 3421–3426

    Article  PubMed  CAS  Google Scholar 

  • Hayashi K, Schoonbeek HJ, de Waard MA (2002) Bcmfs 1, a novel major facilitator superfamily transporter from Botrytis cinerea, provides tolerance towards the natural toxic compounds camptothecin and cercosporin and towards fungicides. Appl Environ Microbiol 68: 4996–5004

    Article  PubMed  CAS  Google Scholar 

  • Hikichi Y, Egami H, Oguri Y, Okuno T (1998) Fitness for survival of Burkholderia glumae resistant to oxolinic acid in rice plants. Ann Phytopathol Soc Jpn 64: 147–152

    CAS  Google Scholar 

  • Hollomon DW (2001) Occurrence and molecular characterization of strobilurin resistance in cucumber powdery mildew and downy mildew. Phytopathology 91: 1166–1171

    Article  PubMed  Google Scholar 

  • Hollomon DW, Butters JA (1994) Molecular determinants for resistance to crop protection chemicals. In: Marshall G, Walters D (eds) Molecular biology in crop protection. Chapman & Hall, London, pp 98–117

    Google Scholar 

  • Ishii H (2002) DNA-based approaches for diagnosis of fungicide resistance. In: Clark JM, Yamaguchi I (eds) Agrochemical resistance: Extent, mechanism and detection. American Chemical Society, Washington DC, pp 242–259

    Google Scholar 

  • Ishii H (2005) Resistance management strategies for fungicides. In: Clark JM, Ohkawa H (eds) Environmental fate and safety management of agrochemicals. American Chemical Society, Washington DC, pp 280–288

    Google Scholar 

  • Ishii H (2006) Impact of fungicide resistance in plant pathogens on crop disease control and agricultural environment. J Agric Res Quart 40: 205–211

    CAS  Google Scholar 

  • Ishii H, Fraaije BA, Sugiyama T, Noguchi K, Nishimura K, Takeda T, Amano T, Hollomon DW (2001) Occurrence and molecular characterization of strobilurin resistance in cucumber powdery mildew and downy mildew. Phytopathology 91: 1166–1171

    Article  CAS  PubMed  Google Scholar 

  • Ishii H, Iwamoto, S, Nishimura K, Fukaya M (1998) Comparative studies on fungicide sensitivity and other characteristics in Colletotrichum isolated from various plant species. Brighton Conf Proc Pests and Diseases pp 529–534

    Google Scholar 

  • Ishii H, Sugiyama T, Nishimura K, Ishikawa Y (2002) Strobilurin resistance in cucumber pathogens: Persistence and molecular diagnosis of resistance. In: Dehne, HW (ed) Modern fungicides and antifungal compounds III. AgroConcept, Bonn, Germany, pp 149–159

    Google Scholar 

  • Ishii H, Tanoue J, Oshima M, Yamaguchi J, Nemoto F, So K (2005) Application of a PCR-Luminex system for molecular diagnosis of Magnaporthe grisea isolates resistant to dehydratase inhibitors in melanin biosynthesis (MBI-D). In: Modern fungicides and antifungal compounds IV. Brighton Crop Protection Conference, Alton, UK, pp 31–34

    Google Scholar 

  • Jeffers SN, Schnabel G, Smith JP (2004) First report of resistance to mefenoxam in Phytophthora cactorum in the United States and elsewhere. Plant Dis 88: 576

    Article  Google Scholar 

  • Judelson HS, Roberts S (1999) Multiple loci determining insensitivity to phenylamide fungicides in Phytophthora infestans. Phytopathology 89: 754–760

    Article  CAS  PubMed  Google Scholar 

  • Kaku K, Takagaki M, Shimizu T, Nagayama K (2003) Diagnosis of dehydratase inhibitors in melanin biosynthesis inhibitor (MBI-D) resistance by primer-introduced restriction enzyme analysis in scytalone dehydratase gene of Magnaporthe grisea. Pest Manag Sci 59: 843–846

    Article  PubMed  CAS  Google Scholar 

  • Kim YS, Dixon EW, Vincelli P, Farman ML (2003) Field resistance to strobilurin (QoI) fungicides in Pyricularia grisea caused by mutations in the mitochondrial cytochrome $b$ gene. Phytopathology 93: 891–900

    Article  CAS  PubMed  Google Scholar 

  • Koenraadt H, Somerville SC, Jones AJ (1992) Characterization of mutations in the $\ubeta$-tubulin gene of benomyl-resistant field strains of Venturia inaequalis and other plant pathogenic fungi. Mol Plant Pathol 82: 1348–1354

    Google Scholar 

  • Lee TY, Mizubuti ES, Fry WE (1999) Genetics and metalaxyl resistance in Phytophthora infestans. Fungal Genet Biol 26: 118–130

    Article  PubMed  CAS  Google Scholar 

  • Lee YAM, Hendson M, Panopoulos NJ, Schroth MV (1994) Molecular cloning, chromosomal mapping and sequence-analysis of copper resistance genes from Xanthomonas campestris pv. juglandis: homology with small blue copper proteins and multicopper oxidase. J Bacteriol 176: 173–188

    PubMed  CAS  Google Scholar 

  • Lucas JA, Greer G, Oudemans PV, Coffey MD (1990) Fungicide sensitivity in somatic hybrids of Phytophthora capsici by protoplast fusion. Physiol Mol Plant Pathol 36: 175–187

    Article  CAS  Google Scholar 

  • Luck JE, Gillings MR (1995) Rapid identification of benomyl resistant strains of Botrytis cinerea using polymerase chain reaction. Mycol Res 99: 1483–1488

    Article  CAS  Google Scholar 

  • Maeda Y, Horita M, Shinohara H, Kiba A, Ohnishi K, Tsushima S, Hikichi Y (2007) Analyses of sources of oxolonic acid-resistant field strains of Burkholderia glumae based on rep-PCR analysis and nucleotide sequences of gyrB and rpoD. J Gen Plant Pathol 73: 46–52

    Article  CAS  Google Scholar 

  • Maeda Y, Kiba A, Ohnishi K, Hikichi Y (2004a) Implications of amino acid substitutions in GyrA at position 83 in terms of oxolonic acid resistance in field isolates of Burkholderia glumae, a causal agent of bacterial seedling rot and grain rot of rice. Appl Environ Microbiol 70: 5613–5620

    Article  CAS  Google Scholar 

  • Maeda Y, Kiba A, Ohnishi K, Hikichi Y (2004b) New method to detect oxolonic acid-resistant Burkholderia glumae infesting rice seeds using a mismatch amplification mutation assay polymerase chain reaction. J Gen Plant Pathol 70: 215–217

    Article  CAS  Google Scholar 

  • Martin HL, Hamilton VA, Kopittke RA (2004) Copper tolerance in Australian populations of Xanthomonas axonopodis pv. vesicatoria. Plant Dis 88: 921–924

    Article  CAS  Google Scholar 

  • Maymon M, Zveibil A, Pivonia S, Minz D, Freeman S (2006) Identification and characterization of benomyl-resistant and sensitive populations of Colletotrichum gloeosporioides from statice (Limonium spp.). Phytopathology 96: 542–548

    Article  CAS  PubMed  Google Scholar 

  • McKay GJ, Cooke LR (1997) A PCR-based method to characterize and identify benzimidazole resistance in Helminthosporium solani. FEMS Microbiol Lett 152: 371–378

    Article  PubMed  CAS  Google Scholar 

  • Nakaune R, Adachi K, Nawala O, Tomiyama M, Akutsu K, Hibi T (1998) A novel ATP-binding cassette transporter involved in multidrug resistance in the phytopathogenic fungus Penicillium digitatum. Appl Environ Microbiol 64: 3983–3988

    PubMed  CAS  Google Scholar 

  • Narayanasamy P (2002) Microbial plant pathogens and crop disease management. Science Publishers, Enfield, USA

    Google Scholar 

  • Orth AB, Rzhetskaya M, Pell EJ, Tien M (1995) A serine (threonine) protein kinase confers fungicide resistance in the phytopathogenic fungus Ustilago maydis. Appl Environ Microbiol 61: 2341–2345

    PubMed  CAS  Google Scholar 

  • Orth AB, Sfarra A, Pell EJ, Tien M (1994) Characterization and genetic analysis of laboratory mutants of Ustilago maydis resistant to dicarboximide and aromatic hydrocarbon fungicides. Phytopathology 84: 1210–1214

    Article  CAS  Google Scholar 

  • Paluh JL, Killilea AN, Detrich HWIII, Downing KH (2004) Meiosis-specific failure of cell cycle progression in fission yeast by mutation of a conserved $\ubeta$-tubulin residue. Mol Biol Cell 15: 1160–1171

    Article  PubMed  CAS  Google Scholar 

  • Peres NAR, Souza NL, Peever TL, Timmer LW (2004) Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus. Plant Dis 88: 125–130

    Article  CAS  Google Scholar 

  • Proffer TJ, Berardi R, Ma Z, Nugent JE, Ehret GR, Mc Manus PS, Jones AL, Sundin GW (2006) Occurrence, distribution and polymerase chain reaction-based detection of resistance to sterol demethylation inhibitor fungicides in populations of Blumeriella jaapii in Michigan. Phytopathology 96: 709–717

    Article  CAS  PubMed  Google Scholar 

  • Reimann S, Deising HB (2005) Inhibition of efflux transporter-mediated fungicide resistance in Pyrenophora tritici-repentis by a derivative of $4^′$-hydroxyflavone and enhancement of fungicide activity. Appl Environ Microbiol 71: 3269–3275

    Article  PubMed  CAS  Google Scholar 

  • Rothamsted Research (2003–2004) Rothamsted Annual Report for 2003–2004, Rothamsted Research Station UK, pp 13–17

    Google Scholar 

  • Saurin W, Hofnung M, Dassa E (1999) Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters. J Mol Evol 48: 22–41

    Article  PubMed  CAS  Google Scholar 

  • Sawada J, Sugihara M, Takagaki M, Nagayama K (2004) Monitoring and characterization of Magnaporthe grisea isolates with decreased sensitivity to scytalone-dehydratase inhibitors. Pest Manag Sci 60: 777–785

    Article  PubMed  CAS  Google Scholar 

  • Schmidt LS, Ghosoph JM, Margosan DA, Smilanick JL (2006) Mutation at $\ubeta$-tubulin codon 200 indicated thiabendazole resistance in Penicillium digitatum collected from California citrus packinghouses. Plant Dis 90: 765–770

    Article  CAS  Google Scholar 

  • Schnabel G, Jones AL (2001) The alpha-demethylase (CYP51A1) gene is overexpressed in Venturia inaequalis strains resistant to myclobuatnil. Phytopathology 91: 102–110

    Article  CAS  PubMed  Google Scholar 

  • Schroth MN Beutti JA, Moller WJ, Reil WO (1969) Fire blight of pears in California: current status and research progress. California Plant Pathol 7: 1–5

    Google Scholar 

  • Shaw DS, Shattock RC (1991) Genetics of Phytophthora infestans: the Mendelian approach. In: Lucas JA, Shattock RC, Shaw DS, Cooke LR (eds) Phytophthora. Cambridge University Press, Cambridge, UK, pp 218–230

    Google Scholar 

  • Siebert PD, Chenchik A, Kellog DE, Lukyanov KA, Lukyanove SA (1995) An improved method of walking in uncloned genomic DNA. Nucleic Acids Res 23: 1087–1088

    Article  PubMed  CAS  Google Scholar 

  • Spotts RA, Cervantes LA (1995) Copper, oxytetracycline and streptomycin resistance of Pseudomonas syringae pv. syringae strains from pear orchards in Oregon and Washington. Plant Dis 79: 1132–1135

    CAS  Google Scholar 

  • Stergiopoulos I, van Nistelrooy JGM, Kema GHJ, De Waard MA (2003) Multiple mechanisms account for variation in base-line sensitivity to azole fungicides in field isolates of Mycosphaerella graminicola. Pest Manag Sci 59: 1333–1343

    Article  PubMed  CAS  Google Scholar 

  • Voloudakis AE, Bender CL, Cooksey DA (1993) Similarity between copper resistance genes from Xanthomonas campestris and Pseudomonas syringae. Appl Environ Microbiol 59: 1627–1634

    PubMed  CAS  Google Scholar 

  • Voloudakis AE, Reignier TM, Cooksey DA (2005) Regulation of resistance to copper in Xanthomonas axonopodis pv. vesicatoria. Appl Environ Microbiol 71: 782–789

    Article  PubMed  CAS  Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 12: 6531–6535

    Article  Google Scholar 

  • Yamaguchi, J, Kuchiki F, Hirayae K, So K (2002) Decreased effect of carpropamid for rice blast control in the west north area of Saga prefecture in 2001. Jpn J Phytopathol 68:261 (abst.)

    Google Scholar 

  • Zhan J, Stefanato FL, McDonald BA (2006) Selection for increased cyproconazole tolerance in Mycosphaerella graminicola through local adaptation and in response to host resistance. Mol Plant Pathol 7: 259–268

    Article  CAS  Google Scholar 

  • Zwiers LH, Sterigopoulos I, Gielkens MM, Goodall, SD, de Waard MA (2003) ABC transporters of wheat pathogen Mycosphaerella graminicola function as protectants against biotic and xenobiotic toxic compounds. Mol Genet Genome 269: 499–507

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer Science+Business Media B.V.

About this chapter

Cite this chapter

Narayanasamy, P. (2008). Molecular Biology of Pathogen Resistance to Chemicals. In: Molecular Biology in Plant Pathogenesis and Disease Management. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8247-4_7

Download citation

Publish with us

Policies and ethics