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Early Steps of V(D)J Rearrangement: Insights from Biochemical Studies of RAG-RSS Complexes

  • Chapter
V(D)J Recombination

Part of the book series: Advances in Experimental Medicine and Biology ((volume 650))

Abstract

V(D)J recombination is initiated by the synapsis and cleavage of a complementary (12/23) pair of recombination signal sequences (RSSs) by the RAG1 and RAG2 proteins. Our understanding of these processes has been greatly aided by the development of in vitro biochemical assays of RAG binding and cleavage activity. Accumulating evidence suggests that synaptic complex assembly occurs in a step-wise manner and that the RAG proteins catalyze RSS cleavage by mechanisms similar to those used by bacterial transposases. In this chapter we will review the molecular mechanisms of RAG synaptic complex assembly and 12/23-regulated RSS cleavage, focusing on recent advances that shed new light on these processes.

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Authors and Affiliations

  1. Department of Medical Microbiology and Immunology, Creighton University Medical Center, Omaha, Nebraska, USA

    Patrick C. Swanson, Sushil Kumar & Prafulla Raval

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  1. Patrick C. Swanson
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  2. Sushil Kumar
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  3. Prafulla Raval
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Correspondence to Patrick C. Swanson .

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  1. Centre d’Immunologie de Marseille-Luminy, Université d’Aix Marseille, Marseille, France

    Pierre Ferrier MD, PhD

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Swanson, P.C., Kumar, S., Raval, P. (2009). Early Steps of V(D)J Rearrangement: Insights from Biochemical Studies of RAG-RSS Complexes. In: Ferrier, P. (eds) V(D)J Recombination. Advances in Experimental Medicine and Biology, vol 650. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-0296-2_1

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