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Genetics, Plants, and the Polymerase Chain Reaction

  • Chapter
The Polymerase Chain Reaction

Abstract

The polymerase chain reaction (PCR) has given plant geneticists, ecologists, evolutionary, and population biologists a powerful new tool for studying their favorite organisms. In this chapter, we will use specific PCR to mean a standard, two-primer amplification that has as a target a specific genomic region, or gene, and therefore requires specific primers to be designed based on knowledge of DNA sequence. We differentiate this from PCR that uses primers of arbitrary sequence to specifically amplify a set of arbitrary loci in any genome, without the requirement for prior sequence knowledge. This is usually referred to as arbitrarily primed PCR or random amplified polymorphic DNA (RAPD) markers; herein, we will use the term arbitrarily primed PCR.

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Authors
  1. Bruno W. S. Sobral
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  2. Rhonda J. Honeycutt
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Editor information

Editors and Affiliations

  1. La Jolla, CA, 92037, USA

    Kary B. Mullis

  2. The Immune Response Corporation, 5935 Darwin Court, Carlsbad, CA, 92008, USA

    François Ferré

  3. Institute for Molecular Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA

    Richard A. Gibbs

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Sobral, B.W.S., Honeycutt, R.J. (1994). Genetics, Plants, and the Polymerase Chain Reaction. In: Mullis, K.B., Ferré, F., Gibbs, R.A. (eds) The Polymerase Chain Reaction. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4612-0257-8_26

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  • DOI: https://doi.org/10.1007/978-1-4612-0257-8_26

  • Publisher Name: Birkhäuser, Boston, MA

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  • Online ISBN: 978-1-4612-0257-8

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