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Fermentations of Pectin-Rich Biomass with Recombinant Bacteria to Produce Fuel Ethanol

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Twenty-First Symposium on Biotechnology for Fuels and Chemicals

Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

Abstract

Pectin-rich residues from sugar beet processing contain significant carbohydrates and insignificant amounts of lignin. Beet pulp was evaluated for conversion to ethanol using recombinant bacteria as biocatalysts. Hydrolysis of pectin-rich residues followed by ethanolic fermentations by yeasts has not been productive because galacturonic acid and arabinose are not fermentable to ethanol by these organisms. The three recombinant bacteria evaluated in this study, Escherichia coli strain KO11, Klebsiella oxytoca strain P2, and Erwinia chrysanthemi EC 16 pLOI 555, ferment carbohydrates in beet pulp with varying efficiencies. E. coli KO11 is able to convert pure galacturonic acid to ethanol with minimal acetate production. Using an enzyme loading of 10.5 filter paper units of cellulase, 120.4 polygalacturonase units of pectinase, and 6.4 g of cellobiase (per gram of dry wt sugar beet pulp), with substrate addition after 24 h of fermentation, 40 g of ethanol/L was produced. Other recombinants exhibited lower ethanol yields with increases in acetate and succinate production.

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Authors and Affiliations

  1. Department of Biology, Central Michigan University, 217 Brooks Hall, Mt. Pleasant, MI, 48859, USA

    Joy Bethune Doran, Jennifer Cripe, Misty Sutton & Brian Foster

Authors
  1. Joy Bethune Doran
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  2. Jennifer Cripe
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  3. Misty Sutton
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  4. Brian Foster
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Corresponding author

Correspondence to Joy Bethune Doran .

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Editors and Affiliations

  1. National Renewable Energy Laboratory, USA

    Mark Finkelstein

  2. Oak Ridge National Laboratory, USA

    Brian H. Davison

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© 2000 Springer Science+Business Media New York

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Doran, J.B., Cripe, J., Sutton, M., Foster, B. (2000). Fermentations of Pectin-Rich Biomass with Recombinant Bacteria to Produce Fuel Ethanol. In: Finkelstein, M., Davison, B.H. (eds) Twenty-First Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1392-5_10

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  • DOI: https://doi.org/10.1007/978-1-4612-1392-5_10

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-7128-4

  • Online ISBN: 978-1-4612-1392-5

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