Abstract
Bacillus subtilis S499 is well-known for its ability to produce two families of surfactant lipopeptides: Iturin A and Surfactin S1. Fermentation optimization for this strain was performed to amplify the surfactant production. Ten active variables were analyzed by two successive Plackett-Burman designs, consisting respectively of 12 and 16 experiments to give an optimized medium. The amount of biosurfactant lipopeptides in the supernatant of a culture carried out in this optimized medium was about five times higher than that obtained in nonoptimized rich medium. The analysis of the surfactant molecules produced in such optimized conditions has revealed the presence of a third family of lipopeptides: the fengycins.
The time-dependent production of these three families of molecules in bioreactors showed that surfactin SI is produced during the exponential phase and iturin A and fengycins during the stationary phase.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Desai, J. D. and Banat, I. M. (1997), Microbiol. Mol. Biol. Rev. 61, 47–64.
Sandrin, C, Peypoux, F., and Michel, G. (1990), Biotechnol. Appl. Biochem. 12, 370–375.
Peypoux, F., Bonmatin, J. M., Labbe, H., Das, B. C, Ptak, M., and Michel, G. (1991), Eur. J. Biochem. 202, 101–106.
Besson, F., Hourdou, M. L., and Michel, G. (1990), Biochem. Biophys. Acta 1036, 101–106.
Oka, K., Hirano, T., Homma, M., Ishii, H., Murakami, K., Mogami, S., Motizuki, A., Morita, H., Takeya, K., and Itokawa, H. (1993), Chem. Pharm. Bull. 41, 1000–1002.
Isogai, A., Takayama, S., Murakoshi, S., and Suzuki, A. (1982), Tetrahedron Lett. 23, 3065–3068.
Arima, K., Kakinuma, A., and Tamura, G. (1968), Biochem. Biophys. Res. Commun. 31, 488–494.
Razafindralambo, H., Paquot, M., Baniel, A., Popineau, Y., Hbid C, Jacques, Ph., and Thonart, Ph. (1996), J. Am. Oil Chem. Soc. 73, 149–151.
Razafindralambo, H., Paquot, M., Baniel, A., Popineau, Y., Hbid C, Jacques, Ph., and Thonart, Ph. (1997), Food Hydrocoll. 11, 59–62.
Vollenbroich, D., Pauli, G., Ozel, M., and Vater, J. (1997), Appl. Environ. Microbiol. 63, 44–49.
Vollenbroich, D., Ozel, M., Vater, J., Kamp, R. M., and Pauli, G. (1997), Biologicals 25, 289–297.
Besson, F., Peypoux, F., and Michel, G. (1979), J. Antibiot. 32, 828–833.
Thimon, L., Peypoux, F., Maget-Dana, R., Roux B., and Michel, G. (1992), Biotechnol. Appl. Biochem. 16, 144–151.
Razafindralambo, H., Popineau, Y., Deleu, M., Hbid, C, Jacques, Ph., Thonart, Ph., and Paquot, M. (1997), Langmuir 13, 6026–6031.
Plackett, R. L. and Burman, J. P. (1946), Biometrika 33, 305–325.
Delcambe, L. (1965), Bull. Soc. Chim. Belg. 74, 315–328.
Razafindralambo, H., Paquot, M., Hbid, C, Jacques, Ph., Destain, J., and Thonart, Ph. (1993), J. Chromatogr. 639, 81–85.
Quentin, M. J., Besson, F., Peypoux, F., and Michel, G. (1982), Biochim. Biophys. Acta 684, 207–211.
Maget-Dana, R., Thimon, L., Peypoux, F., and Ptak, M. (1992), Biochimie 74, 1047–1051.
Vanittanakom, N., Loeffler, W., Koch, U., and Jung, G. J. (1986), J. Antibiot. 39, 888–901.
Mulligan, C. N., Chow, T. Y. K., and Gibbs, B. F. (1989), Appl. Microbiol. BioTechnol. 31, 486–489.
Hbid, C., Jacques, Ph., Razafindralambo, H., Mpoyo, M. K., Meurice, E., Paquot, M., and Thonart, Ph. (1996), Appl. Biochem. BioTechnol. 57/58, 571–579.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media New York
About this chapter
Cite this chapter
Jacques, P. et al. (1999). Optimization of Biosurfactant Lipopeptide Production from Bacillus subtilis S499 by Plackett-Burman Design. In: Davison, B.H., Finkelstein, M. (eds) Twentieth Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1604-9_22
Download citation
DOI: https://doi.org/10.1007/978-1-4612-1604-9_22
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4612-7214-4
Online ISBN: 978-1-4612-1604-9
eBook Packages: Springer Book Archive