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Mechanisms of Cell Fusion and Selection in the Generation of Hybridomas

  • Chapter
Methods of Hybridoma Formation

Part of the book series: Contemporary Biomedicine ((CB,volume 7))

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Abstract

The hybridoma technology for the production of monoclonal antibodies was initially reported by Kohler and Milstein in 1975 (1), but its discovery was actually the culmination of advances in several unrelated fields of research. In this review the various cell fusion and selection techniques that allowed for the development of the hybridoma technology will be considered. In addition, some recent advances in cell hybridization and selection procedures that may be useful in enhancing the production of hybridomas will be discussed.

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Authors
  1. Jerry W. Shay
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Editor information

Editors and Affiliations

  1. Biotherapeutics, Inc., Franklin, Tennessee, USA

    Arie H. Bartal

  2. Hybridoma Laboratory, Northern Israel Oncology Center, Rambam Medical Center, Haifa, Israel

    Arie H. Bartal

  3. Sloan-Kettering Cancer Center, New York, New York, USA

    Yashar Hirshaut

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© 1987 The Humana Press Inc.

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Shay, J.W. (1987). Mechanisms of Cell Fusion and Selection in the Generation of Hybridomas. In: Bartal, A.H., Hirshaut, Y. (eds) Methods of Hybridoma Formation. Contemporary Biomedicine, vol 7. Humana Press. https://doi.org/10.1007/978-1-4612-4826-2_3

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  • DOI: https://doi.org/10.1007/978-1-4612-4826-2_3

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-9179-4

  • Online ISBN: 978-1-4612-4826-2

  • eBook Packages: Springer Book Archive

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