Abstract
The cytostatic agent cis-diamminedichloroplatinum(II) (cisplatin) can react with DNA and RNA as well as with proteins. It is generally assumed that the antineoplastic activity results from the interaction with DNA inside the cell nucleus. Therefore, studies on the induction and repair of cisplatin-DNA adducts (e.g. in cultured cells) have been and are being performed to get insight into the working mechanism of cisplatin. In Fig.1 the various types of reaction products are depicted. Despite a multitude of investigations in the last 10 years, it is still unknown which of the adducts is responsible for the antitumor action or for the (undesired) side effects of the drug. An important bottleneck in these studies was the lack of analytical methods for the quantitative detection of the DNA adducts at a level of sensitivity and selectivity that would permit their determination in DNA of mildly exposed cells or tissues.
The known cisplatin-DNA adducts.
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Fichtinger-Schepman, A.M.J., Dijt, F.J., De Jong, W.H., Van Oosterom, A.T., Berends, F. (1988). In Vivo Cis-Diamminedichloroplatinum(II)-DNA Adduct Formation and Removal as Measured with Immunochemical Techniques. In: Nicolini, M. (eds) Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy. Developments in Oncology, vol 54. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1717-3_3
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DOI: https://doi.org/10.1007/978-1-4613-1717-3_3
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