Abstract
With the advent of translucent acrylic plastic cells, it has become apparent that sizable and variable volume shifts occur across a semipermeable membrane during equilibrium dialysis. These volume shifts, toward the plasma or protein side, influence the estimation of binding parameters and the unbound fraction. A method of correcting for volume shifts is presented using binding data for salicylate and prednisolone. The specific dialysis procedures employed have been described elsewhere (1,2).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
D.E. Furst, T.N. Tozer and K.L. Melmon, Salicylate clearance, the resultant of protein binding and metabolism, Clin. Pharmacol. Ther. 26: 380 – 389 (1979).
J.G. Gambertoglio, F.J. Frey, N.H.G. Holford, J.L. Birnbaum, P.S. Lisak, F. Vincenti, N.J. Feduska, O. Salvatierra and W.J.C. Amend, Prednisone and prednisolone bioavailability in renal transplant patients, Kidney Int. 21: 621 – 626 (1982).
T.N. Tozer, J.G. Gambertoglio, D.E. Furst, D.S. Avery and N.H.G. Holford, Volume shifts and protein binding estimates using equilibrium dialysis - application to prednisolone binding in man, J. Pharm. Sci. 72: 1442 – 1446 (1983).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Tozer, T.N. (1984). Protein Binding Estimates in the Presence of Volume Shifts During Equilibrium Dialysis. In: Benet, L.Z., Levy, G., Ferraiolo, B.L. (eds) Pharmacokinetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2799-8_57
Download citation
DOI: https://doi.org/10.1007/978-1-4613-2799-8_57
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9725-3
Online ISBN: 978-1-4613-2799-8
eBook Packages: Springer Book Archive