Abstract
The purpose of this chapter is to become familiar with legumes in the field, examine their nodules, isolate rhizobia from nodules, and preserve the isolates. The subfamilies in the Leguminosae will be discussed and identifications will be made with the help of a botanical key. Nodules will be sectioned and examined. Simple stains of nodule smears will be examined under the microscope. Rhizobia will be isolated from nodules and grown on presumptive test media. The isolates will be authenticated on their original host plants and then preserved on ceramic beads.
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Key References
Date, R.A. 1982. Collection, isolation, characterisation and conservation of Rhizobium. pp. 95–109. In J.M. Vincent (ed.) Nitrogen Fixation in Legumes. Academic Press, Sydney.
Fred, E.B., I.L. Baldwin, and E. McCoy. 1932. Root Nodule Bacteria and Leguminous Plants, University of Wisconsin, Madison.
Jordan, D.C. 1984. Rhizobiaceae. pp. 234–256. In N.R. Krieg and J.G. Holt (eds.) M. Bergey’s Manual of Systematic Bacteriology, Vol. I. The Williams & Wilkins Co., Baltimore.
Vincent, J.M. 1970. A Manual for the Practical Study of Root Nodule Bacteria. IBP Handbook no. 15. Blackwell Scientific Publications, Oxford.
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© 1994 Springer-Verlag New York, Inc.
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Somasegaran, P., Hoben, H.J. (1994). Collecting Nodules and Isolating Rhizobia. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_1
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DOI: https://doi.org/10.1007/978-1-4613-8375-8_1
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-8377-2
Online ISBN: 978-1-4613-8375-8
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