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HIV-1 Genotyping and Virtual Phenotyping

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Modern Clinical Molecular Techniques

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Abstract

Drug resistance testing is recommended for the management of patients with HIV-1 infection. Celera ViroSeq™ HIV-1 Genotyping System is the most widely used genotypic HIV drug resistance testing. The assay produces 1,302 nucleotide sequences comprising codons 1–99 of the protease gene and 1–335 of the reverse transcriptase gene. Genotyping result is linked to an interpretive algorithm, for example, Virco® TYPE HIV-1 to generate drug resistance profile.

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Acknowledgment

We would like to thank Dr. Natalia M. Marlowe for critical review and comments of the chapter.

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Authors and Affiliations

  1. Division of Molecular Diagnostics, Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX, 77555-0743, USA

    Fangling Xu M.D., Ph.D., MB(ASCP) CM & Jianli Dong M.D., Ph.D.

Authors
  1. Fangling Xu M.D., Ph.D., MB(ASCP) CM
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  2. Jianli Dong M.D., Ph.D.
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Corresponding author

Correspondence to Jianli Dong M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. , Molecular Genetic Technology Program, M.D. Anderson Cancer Center, 1515 Holcombe Blvd. Unit 2, Houston, 77030, Texas, USA

    Peter Hu

  2. , Department of Human Genetics, Emory University School of Medicine, 615 Michael St. NE, Suite 315, Atlanta, 30322, Georgia, USA

    Madhuri Hegde

  3. , Molecular Genetic Technology Program, M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Unit 2, Houston, 77030, Texas, USA

    Patrick Alan Lennon

Additional information

Disclaimer: The contents of this chapter represent modified ViroSeq protocol which has been validated at the Department of Pathology, University of Texas Medical Branch

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© 2012 Springer Science+Business Media, LLC

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Xu, F., Dong, J. (2012). HIV-1 Genotyping and Virtual Phenotyping. In: Hu, P., Hegde, M., Lennon, P. (eds) Modern Clinical Molecular Techniques. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2170-2_8

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  • DOI: https://doi.org/10.1007/978-1-4614-2170-2_8

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-2169-6

  • Online ISBN: 978-1-4614-2170-2

  • eBook Packages: MedicineMedicine (R0)

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