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Liquid Chromatography-Mass Spectrometry for the Determination of Antidepressants and Some of their Major Metabolites in Human Biological Matrices

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LC-MS in Drug Bioanalysis

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Abstract

Due to the high prescription of antidepressants and their frequent involvement in clinical and forensic intoxications, reliable analytical techniques for identification and quantification of these therapeutic drugs should be available in clinical and toxicological laboratories. Improvements in liquid chromatography-mass spectrometry LC-MS(MS) technology over the last two decades have favored this technique to be one of the most commonly employed for this purpose, as it combines the high selectivity and sensitivity of the mass spectrometer with the great versatility of the liquid chromatographic separation. In this chapter, LC-MS(MS) applications for antidepressants determination are reviewed, detailing typical sample preparation techniques used for these therapeutic drugs, as well as common chromatographic and mass spectrometric characteristics. In addition, an LC-MS/MS method for the most common antidepressants used in clinical practice is described as an example of a whole method development and validation.

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Authors and Affiliations

  1. Forensic Toxicology Service, Institute of Forensic Sciences, University of Santiago de Compostela, 15782, Santiago de Compostela, Spain

    Ana de Castro, Angelines Cruz & Manuel López-Rivadulla

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  1. Ana de Castro
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  2. Angelines Cruz
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  3. Manuel López-Rivadulla
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Correspondence to Manuel López-Rivadulla .

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  1. , Department of Experimental Therapeutics, University of Texas MD Anderson Cancer C, El Rio Street 8000, Houston, 77054, USA

    Q. Alan Xu

  2. , Department of Experimental Therapeutics, University of Texas MD Anderson Cancer C, El Rio Street 8000, Houston, 77054, USA

    Timothy L. Madden

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de Castro, A., Cruz, A., López-Rivadulla, M. (2012). Liquid Chromatography-Mass Spectrometry for the Determination of Antidepressants and Some of their Major Metabolites in Human Biological Matrices. In: Xu, Q., Madden, T. (eds) LC-MS in Drug Bioanalysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-3828-1_7

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