Abstract
Transcriptomics (gene expression profiling) refers to the quantitative and qualitative characterization of the collection of ribose nucleic acid (RNA) elements expressed in a biological system and represents one of the first truly genome-wide hypothesis-free investigative approaches in molecular biology. The advent of synthetic oligonucleotide microarray technologies has enabled large-scale application of gene expression profiling in the study of human disease, particularly malignant and hematological processes. Due to favorable characteristics of these processes, including their involvement of one cellular compartment (and often a specific, monoclonal cell type), the severity of the underlying cellular perturbation under study (malignant vs. benign cells), and the accessibility to large numbers of available banked samples obtained during clinically indicated medical procedures, the study of transcriptomics in oncology has been quite fruitful, with notable translation of these techniques to novel clinical applications with diagnostic, prognostic, and therapeutic implications. Furthermore, the discovery of large populations of noncoding RNA elements, including microRNA and long-intergenic noncoding RNA (LINCC-RNA) has expanded the scope of transciptomic profiling beyond the protein-coding messenger RNAs (mRNA).
In this chapter, we provide a brief survey of prior applications of this approach to the study of asthma, followed by an overview of the primary technical and analytical considerations that should be addressed when conducting such studies. For more detailed review of study protocols and specific analytical platforms, readers are referred to several recent publications (Matson 2009; Yakovlev et al. 2013; Dehmer et al. 2012; Rodriguez-Ezpelete et al. 2012).
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Sordillo, J., Raby, B.A. (2014). Gene Expression Profiling in Asthma. In: Brasier, A. (eds) Heterogeneity in Asthma. Advances in Experimental Medicine and Biology, vol 795. Humana Press, Boston, MA. https://doi.org/10.1007/978-1-4614-8603-9_10
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DOI: https://doi.org/10.1007/978-1-4614-8603-9_10
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