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Stable Isotope-Based Dynamic Metabolic Profiling in Disease and Health

Tracer Methods and Applications

  • Chapter
Metabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis

Abstract

Substrates, intermediary metabolites and products, the so called “global metabolite pool”, constitute a living organism’s metabolome. Variations and changes in components of the metabolome reflect adaptation of an organism to its microenvironment, as defined by substrate availability and hormonal milieu, through altered gene expression and through the activation of signaling cascades (Tweeddale et al., 1998). The major regulatory components of cell function, the genome, transcriptome and proteome, ultimately act on the metabolome resulting in the expression of a specific phenotype. The close interactions among these components establish a rationale for integrating functional genomics, proteomics and metabolomics, as a means to study the complete intracellular signal processing apparatus that regulates metabolic adaptation, phenotype and ultimately cellular function (Kell and King, 2000).

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Authors and Affiliations

  1. Harbor-UCLA Research and Education Institute, 1124 West Carson Street RBI, Torrance, CA, 90502, USA

    László G. Boros & Wai-Nang Paul Lee

  2. Department of Biochemistry and Molecular Biology, University of Barcelona, Marti/I Franques 1, Barcelona, 08028, Catalonia, Spain

    Marta Cascante

Authors
  1. László G. Boros
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  2. Marta Cascante
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  3. Wai-Nang Paul Lee
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Editors and Affiliations

  1. Pharmacia Corporation, Chesterfield, MO, 63198, USA

    George G. Harrigan

  2. Department of Chemistry, UMIST, PO Box 88, Manchester, M60 1QD, UK

    Royston Goodacre

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Boros, L.G., Cascante, M., Lee, WN.P. (2003). Stable Isotope-Based Dynamic Metabolic Profiling in Disease and Health. In: Harrigan, G.G., Goodacre, R. (eds) Metabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0333-0_9

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  • DOI: https://doi.org/10.1007/978-1-4615-0333-0_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5025-5

  • Online ISBN: 978-1-4615-0333-0

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