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PON1 Structure

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Paraoxonase (PON1) in Health and Disease

Abstract

Paraoxonase (PON1) is an HDL-bound glycoprotein exhibiting calcium-dependent hydrolase and antioxidative activities, both related to distinct structural domains. Few structural data on PON1 and other related proteins, PON2 and 3, are currently available. The sequence of the 354 amino acid mature enzyme has been deduced from its cDNA. PON1 has 3 Cys residues: Cys-42 and -353 form a disulfide bond, Cys-284 is free. The PON1 hydrophobic N-terminus, predicted as an α-helix, is involved in the association of PON1 with phospholipids. The native enzyme purified from human plasma is not exclusively monomelic. The oligomeric state and size of human PON1 bound to non-ionic detergent molecules depends on the concentration of detergent. Five His (H115, H134, H155, H243, H285), 1 Trp (W281), 2 G1u (E53, E195) and 6 Asp (D54, D169, D183, D269, D279) residues are essential for human PON1 arylesterase and organophosphatase activities. The residues in position 192 and 284, although not essential for the PON1 hydrolase activity, could be close to the active site. PON1 shares common structural and functional features with other lactonohydrolases, mainly PON3. The PON1 3D structure will be established in the near future. It will help to relate PON1 structural and functional properties.

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Author information

Authors and Affiliations

  1. Unité d’enzymologie, Centre de Recherches du Service de Santé des Armées, BP 67, 38702, La Tronche Cedex, France

    Denis Josse & Patrick Masson

  2. Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska, 68198-6805, USA

    Cynthia Bartels & Oksana Lockridge

Authors
  1. Denis Josse
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  2. Patrick Masson
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  3. Cynthia Bartels
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  4. Oksana Lockridge
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Editor information

Editors and Affiliations

  1. Department of Environmental Health, University of Washington, Seattle, WA, 98195-4695, USA

    Lucio G. Costa

  2. Department of Pharmacology and Physiology, University of Roma “La Sapienza”, Roma, Italy

    Lucio G. Costa

  3. Departments of Genome Sciences and Medicine, Division of Medical Genetics, University of Washington, Seattle, WA, 98195-7720, USA

    Clement E. Furlong

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© 2002 Springer Science+Business Media New York

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Josse, D., Masson, P., Bartels, C., Lockridge, O. (2002). PON1 Structure. In: Costa, L.G., Furlong, C.E. (eds) Paraoxonase (PON1) in Health and Disease. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1027-7_2

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  • DOI: https://doi.org/10.1007/978-1-4615-1027-7_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5358-4

  • Online ISBN: 978-1-4615-1027-7

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